skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus

Abstract

This work reports the {sup 13}C-assisted metabolic flux analysis of Haladaptatus paucihalophilus, a halophilic archaeon possessing an intriguing osmoadaption mechanism. We showed that the carbon flow is through the oxidative tricarboxylic acid (TCA) cycle whereas the reductive TCA cycle is not operative in H. paucihalophilus. In addition, both threonine and the citramalate pathways contribute to isoleucine biosynthesis, whereas lysine is synthesized through the diaminopimelate pathway and not through the α-aminoadipate pathway. Unexpected, the labeling patterns of glycine from the cells grown on [1-{sup 13}C]pyruvate and [2-{sup 13}C]pyruvate suggest that, unlike all the organisms investigated so far, in which glycine is produced exclusively from the serine hydroxymethyltransferase (SHMT) pathway, glycine biosynthesis in H. paucihalophilus involves different pathways including SHMT, threonine aldolase (TA) and the reverse reaction of glycine cleavage system (GCS), demonstrating for the first time that other pathways instead of SHMT can also make a significant contribution to the cellular glycine pool. Transcriptional analysis confirmed that both TA and GCS genes were transcribed in H. paucihalophilus, and the transcriptional level is independent of salt concentrations in the culture media. This study expands our understanding of amino acid biosynthesis and provides valuable insights into the metabolism of halophilic archaea. - Highlights: • Serine hydroxymethyltransferase,more » threonine aldolase, and glycine cleavage system all contribute to the glycine pool of H. paucihalophilus. • Threonine and the citramalate pathways contribute equally to the isoleucine biosynthesis in H. paucihalophilus. • Lysine in H. paucihalophilus is synthesized through the diaminopimelate pathway and not through the α-aminoadipate pathway. • Glycine biosynthesis is likely unrelated to the cell osmoadaption mechanism.« less

Authors:
;  [1];  [2];  [3];  [4];  [1];  [2];  [4];  [3];  [1];  [2];  [2]
  1. Key Laboratory of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000 (China)
  2. (China)
  3. Department of Chemistry, Fudan University, Shanghai, 200433 (China)
  4. Key Laboratory of Combinatory Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071 (China)
Publication Date:
OSTI Identifier:
22594106
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 467; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALDOLASES; BIOSYNTHESIS; CARBON 13; CLEAVAGE; CONCENTRATION RATIO; CULTURE MEDIA; GLYCINE; LABELLING; LYSINE; METABOLISM; OXIDATION; SALTS; SERINE; THREONINE

Citation Formats

Liu, Guangxiu, Zhang, Manxiao, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Mo, Tianlu, He, Lian, Zhang, Wei, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Yu, Yi, E-mail: yu_yi@whu.edu.cn, Zhang, Qi, E-mail: qizhang@sioc.ac.cn, Ding, Wei, E-mail: dingw@lzu.edu.cn, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, and Department of Chemistry, Fudan University, Shanghai, 200433. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.09.174.
Liu, Guangxiu, Zhang, Manxiao, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Mo, Tianlu, He, Lian, Zhang, Wei, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Yu, Yi, E-mail: yu_yi@whu.edu.cn, Zhang, Qi, E-mail: qizhang@sioc.ac.cn, Ding, Wei, E-mail: dingw@lzu.edu.cn, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, & Department of Chemistry, Fudan University, Shanghai, 200433. Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus. United States. doi:10.1016/J.BBRC.2015.09.174.
Liu, Guangxiu, Zhang, Manxiao, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Mo, Tianlu, He, Lian, Zhang, Wei, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, Yu, Yi, E-mail: yu_yi@whu.edu.cn, Zhang, Qi, E-mail: qizhang@sioc.ac.cn, Ding, Wei, E-mail: dingw@lzu.edu.cn, Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000, and Department of Chemistry, Fudan University, Shanghai, 200433. Fri . "Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus". United States. doi:10.1016/J.BBRC.2015.09.174.
@article{osti_22594106,
title = {Metabolic flux analysis of the halophilic archaeon Haladaptatus paucihalophilus},
author = {Liu, Guangxiu and Zhang, Manxiao and Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000 and Mo, Tianlu and He, Lian and Zhang, Wei and Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000 and Yu, Yi, E-mail: yu_yi@whu.edu.cn and Zhang, Qi, E-mail: qizhang@sioc.ac.cn and Ding, Wei, E-mail: dingw@lzu.edu.cn and Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Gansu Province, Lanzhou, 730000 and Department of Chemistry, Fudan University, Shanghai, 200433},
abstractNote = {This work reports the {sup 13}C-assisted metabolic flux analysis of Haladaptatus paucihalophilus, a halophilic archaeon possessing an intriguing osmoadaption mechanism. We showed that the carbon flow is through the oxidative tricarboxylic acid (TCA) cycle whereas the reductive TCA cycle is not operative in H. paucihalophilus. In addition, both threonine and the citramalate pathways contribute to isoleucine biosynthesis, whereas lysine is synthesized through the diaminopimelate pathway and not through the α-aminoadipate pathway. Unexpected, the labeling patterns of glycine from the cells grown on [1-{sup 13}C]pyruvate and [2-{sup 13}C]pyruvate suggest that, unlike all the organisms investigated so far, in which glycine is produced exclusively from the serine hydroxymethyltransferase (SHMT) pathway, glycine biosynthesis in H. paucihalophilus involves different pathways including SHMT, threonine aldolase (TA) and the reverse reaction of glycine cleavage system (GCS), demonstrating for the first time that other pathways instead of SHMT can also make a significant contribution to the cellular glycine pool. Transcriptional analysis confirmed that both TA and GCS genes were transcribed in H. paucihalophilus, and the transcriptional level is independent of salt concentrations in the culture media. This study expands our understanding of amino acid biosynthesis and provides valuable insights into the metabolism of halophilic archaea. - Highlights: • Serine hydroxymethyltransferase, threonine aldolase, and glycine cleavage system all contribute to the glycine pool of H. paucihalophilus. • Threonine and the citramalate pathways contribute equally to the isoleucine biosynthesis in H. paucihalophilus. • Lysine in H. paucihalophilus is synthesized through the diaminopimelate pathway and not through the α-aminoadipate pathway. • Glycine biosynthesis is likely unrelated to the cell osmoadaption mechanism.},
doi = {10.1016/J.BBRC.2015.09.174},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 467,
place = {United States},
year = {Fri Nov 27 00:00:00 EST 2015},
month = {Fri Nov 27 00:00:00 EST 2015}
}