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Title: Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation

Abstract

Resveratrol is a sirtuin 1 (SIRT1) activator and can function as an anti-inflammatory and antioxidant factor. In mesenchymal stem cells (MSCs), resveratrol enhances the proliferation and differentiation potential and has an anti-aging effect. However, contradictory effects of resveratrol on MSC cultures have been reported. In this study, we found that resveratrol had different effects on MSC cultures according to their cell passage and SIRT1 expression. Resveratrol enhanced the self-renewal potential and multipotency of early passage MSCs, but accelerated cellular senescence of late passage MSCs. In early passage MSCs expressing SIRT1, resveratrol decreased ERK and GSK-3β phosphorylation, suppressing β-catenin activity. In contrast, in late passage MSCs, which did not express SIRT1, resveratrol increased ERK and GSK-3β phosphorylation, activating β-catenin. We confirmed that SIRT1-deficient early passage MSCs treated with resveratrol lost their self-renewal potential and multipotency, and became senescent due to increased β-catenin activity. Sustained treatment with resveratrol at early passages maintained the self-renewal potential and multipotency of MSCs up to passage 10. Our findings suggest that resveratrol can be effectively applied to early passage MSC cultures, whereas parameters such as cell passage and SIRT1 expression must be taken into consideration before applying resveratrol to late passage MSCs. - Highlights: • Resveratrolmore » enhances self-renewal potential and multipotency of early passage MSCs. • Resveratrol accelerates the cellular senescence of late passage MSCs. • The effects of resveratrol on MSCs are dependent on the presence of SIRT1. • SIRT1 modulates ERK/GSK-3β/β-catenin signaling. • Sustained resveratrol treatment maintains MSC stemness up to P10.« less

Authors:
; ;  [1];  [1]
  1. Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul (Korea, Republic of)
Publication Date:
OSTI Identifier:
22592830
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 467; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AGING; ANTIOXIDANTS; CATTLE; COLONY FORMING UNITS; FIBROBLASTS; GALACTOSIDASE; GLUCOSE; GLYCOGEN; INFLAMMATION; PHOSPHATES; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; STEM CELLS

Citation Formats

Yoon, Dong Suk, Choi, Yoorim, Choi, Seong Mi, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Park, Kwang Hwan, Lee, Jin Woo, E-mail: ljwos@yuhs.ac, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul. Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.10.017.
Yoon, Dong Suk, Choi, Yoorim, Choi, Seong Mi, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Park, Kwang Hwan, Lee, Jin Woo, E-mail: ljwos@yuhs.ac, & Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul. Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation. United States. https://doi.org/10.1016/J.BBRC.2015.10.017
Yoon, Dong Suk, Choi, Yoorim, Choi, Seong Mi, Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Park, Kwang Hwan, Lee, Jin Woo, E-mail: ljwos@yuhs.ac, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul. 2015. "Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation". United States. https://doi.org/10.1016/J.BBRC.2015.10.017.
@article{osti_22592830,
title = {Different effects of resveratrol on early and late passage mesenchymal stem cells through β-catenin regulation},
author = {Yoon, Dong Suk and Choi, Yoorim and Choi, Seong Mi and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul and Park, Kwang Hwan and Lee, Jin Woo, E-mail: ljwos@yuhs.ac and Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul},
abstractNote = {Resveratrol is a sirtuin 1 (SIRT1) activator and can function as an anti-inflammatory and antioxidant factor. In mesenchymal stem cells (MSCs), resveratrol enhances the proliferation and differentiation potential and has an anti-aging effect. However, contradictory effects of resveratrol on MSC cultures have been reported. In this study, we found that resveratrol had different effects on MSC cultures according to their cell passage and SIRT1 expression. Resveratrol enhanced the self-renewal potential and multipotency of early passage MSCs, but accelerated cellular senescence of late passage MSCs. In early passage MSCs expressing SIRT1, resveratrol decreased ERK and GSK-3β phosphorylation, suppressing β-catenin activity. In contrast, in late passage MSCs, which did not express SIRT1, resveratrol increased ERK and GSK-3β phosphorylation, activating β-catenin. We confirmed that SIRT1-deficient early passage MSCs treated with resveratrol lost their self-renewal potential and multipotency, and became senescent due to increased β-catenin activity. Sustained treatment with resveratrol at early passages maintained the self-renewal potential and multipotency of MSCs up to passage 10. Our findings suggest that resveratrol can be effectively applied to early passage MSC cultures, whereas parameters such as cell passage and SIRT1 expression must be taken into consideration before applying resveratrol to late passage MSCs. - Highlights: • Resveratrol enhances self-renewal potential and multipotency of early passage MSCs. • Resveratrol accelerates the cellular senescence of late passage MSCs. • The effects of resveratrol on MSCs are dependent on the presence of SIRT1. • SIRT1 modulates ERK/GSK-3β/β-catenin signaling. • Sustained resveratrol treatment maintains MSC stemness up to P10.},
doi = {10.1016/J.BBRC.2015.10.017},
url = {https://www.osti.gov/biblio/22592830}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 467,
place = {United States},
year = {Fri Nov 27 00:00:00 EST 2015},
month = {Fri Nov 27 00:00:00 EST 2015}
}