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Title: Delocalized Claudin-1 promotes metastasis of human osteosarcoma cells

Abstract

Tight junction proteins (TJPs) including Claudins, Occludin and tight junction associated protein Zonula occludens-1 (ZO-1), are the most apical component of junctional complex that mediates cell–cell adhesion in epithelial and endothelial cells. In human malignancies, TJPs are often deregulated and affect cellular behaviors of tumor cells. In this study, we investigated alternations of TJPs and related biological characteristics in human osteosarcoma (OS). Claudin1 was increased in the metastatic OS cells (KRIB and KHOS) compared with the normal osteoblast cells (hFOB1.19) or primary tumor cells (HOS and U2OS), whereas no significant difference was found in Occludin and ZO-1. Immunohistochemistry, immunofluorescence and Western blotting revealed that Claudin1 was initially localized at cell junctions of normal osteoblasts, but substantially delocalized to the nucleus of metastatic OS cells. Phenotypically, inhibition of the nucleus Claudin1 expression compromised the metastatic potential of KRIB and KHOS cells. Moreover, we found that protein kinase C (PKC) but not PKA phosphorylation influenced Claudin1 expression and cellular functions, as PKC inhibitor (Go 6983 and Staurosporine) or genetic silencing of PKC reduced Claudin1 expression and decreased the motility of KRIB and KHOS cells. Taken together, our study implied that delocalization of claudin-1 induced by PKC phosphorylation contributes to metastatic capacity ofmore » OS cells. - Highlights: • Claudin1 is increased during the malignant transformation of human OS. • Delocalization of Claudin1 in metastatic OS cells. • Silencing nuclear Claudin1 expression inhibits cell invasion of OS. • Deregulated Claudin1 is regulated by PKC.« less

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22592764
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 466; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CONNECTIVE TISSUE CELLS; CONNECTORS; INHIBITION; METASTASES; OSTEOSARCOMAS; PHOSPHORYLATION; PROTEINS; TUMOR CELLS

Citation Formats

Jian, Yuekui, Chen, Changqiong, Li, Bo, and Tian, Xiaobin, E-mail: drtxb_guiyang@sina.com. Delocalized Claudin-1 promotes metastasis of human osteosarcoma cells. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.09.028.
Jian, Yuekui, Chen, Changqiong, Li, Bo, & Tian, Xiaobin, E-mail: drtxb_guiyang@sina.com. Delocalized Claudin-1 promotes metastasis of human osteosarcoma cells. United States. doi:10.1016/J.BBRC.2015.09.028.
Jian, Yuekui, Chen, Changqiong, Li, Bo, and Tian, Xiaobin, E-mail: drtxb_guiyang@sina.com. Fri . "Delocalized Claudin-1 promotes metastasis of human osteosarcoma cells". United States. doi:10.1016/J.BBRC.2015.09.028.
@article{osti_22592764,
title = {Delocalized Claudin-1 promotes metastasis of human osteosarcoma cells},
author = {Jian, Yuekui and Chen, Changqiong and Li, Bo and Tian, Xiaobin, E-mail: drtxb_guiyang@sina.com},
abstractNote = {Tight junction proteins (TJPs) including Claudins, Occludin and tight junction associated protein Zonula occludens-1 (ZO-1), are the most apical component of junctional complex that mediates cell–cell adhesion in epithelial and endothelial cells. In human malignancies, TJPs are often deregulated and affect cellular behaviors of tumor cells. In this study, we investigated alternations of TJPs and related biological characteristics in human osteosarcoma (OS). Claudin1 was increased in the metastatic OS cells (KRIB and KHOS) compared with the normal osteoblast cells (hFOB1.19) or primary tumor cells (HOS and U2OS), whereas no significant difference was found in Occludin and ZO-1. Immunohistochemistry, immunofluorescence and Western blotting revealed that Claudin1 was initially localized at cell junctions of normal osteoblasts, but substantially delocalized to the nucleus of metastatic OS cells. Phenotypically, inhibition of the nucleus Claudin1 expression compromised the metastatic potential of KRIB and KHOS cells. Moreover, we found that protein kinase C (PKC) but not PKA phosphorylation influenced Claudin1 expression and cellular functions, as PKC inhibitor (Go 6983 and Staurosporine) or genetic silencing of PKC reduced Claudin1 expression and decreased the motility of KRIB and KHOS cells. Taken together, our study implied that delocalization of claudin-1 induced by PKC phosphorylation contributes to metastatic capacity of OS cells. - Highlights: • Claudin1 is increased during the malignant transformation of human OS. • Delocalization of Claudin1 in metastatic OS cells. • Silencing nuclear Claudin1 expression inhibits cell invasion of OS. • Deregulated Claudin1 is regulated by PKC.},
doi = {10.1016/J.BBRC.2015.09.028},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 466,
place = {United States},
year = {2015},
month = {10}
}