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Title: Isolation and characterization of a novel mycovirus from Penicillium digitatum

Abstract

A novel double-stranded RNA virus designated Penicillium digitatum virus 1 (PdV1) was isolated from the citrus fruit rot pathogen P. digitatum (HS-RH1). The full-length cDNA sequence of the dsRNA/PdV1 (5211 bp) possesses two partially overlapping open reading frames, which encode a coat protein (CP) and a putative RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis based on multiple alignments of the amino acid sequences of the RdRp and CP indicated that PdV1 tentatively belongs to the genus Victorivirus in the Totiviridae family. Electron micrographs of negatively stained viral particles purified from the peak fraction of sucrose density gradient centrifugation showed spherical particles ~35 nm in diameter. Transfection experiments with purified virions indicated that PdV1 could reduce the vegetative growth and virulence of P. digitatum strain HS-F6. In summary, we report the first isolation and characterization of a mycovirus from P. digitatum that contributes to the hypovirulence phenotypes of the host strain. - Highlights: • A novel victorivirus designated Penicillium digitatum virus 1 (PdV1) was isolated fromP. digitatum. • The dsRNA genome of PdV1 are 5211 bp long, two ORFs encoding CP and RdRp, and are encased in virions of ~35 nm in diameter. • PdV1 infection led to hypovirulent effect onmore » P. digitatum. PdV1 may potentially be used for citrus green mold biocontrol. • Our study provides a research basis for establishing a model system for the study of P. digitatum–mycovirus interactions.« less

Authors:
;  [1];  [2]; ; ;  [1];  [1]
  1. Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Science, Central China Normal University, Wuhan 430079 (China)
  2. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072 (China)
Publication Date:
OSTI Identifier:
22581694
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 494; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; AMINO ACIDS; CITRUS; FRUITS; PENICILLIUM; PROTEINS; RNA; SACCHAROSE; VIRUSES

Citation Formats

Niu, Yuhui, Zhang, Tingfu, Zhu, Ying, Yuan, Yongze, Wang, Shengqiang, Liu, Jing, and Liu, Deli, E-mail: ldl@mail.ccnu.edu.cn. Isolation and characterization of a novel mycovirus from Penicillium digitatum. United States: N. p., 2016. Web. doi:10.1016/J.VIROL.2016.04.004.
Niu, Yuhui, Zhang, Tingfu, Zhu, Ying, Yuan, Yongze, Wang, Shengqiang, Liu, Jing, & Liu, Deli, E-mail: ldl@mail.ccnu.edu.cn. Isolation and characterization of a novel mycovirus from Penicillium digitatum. United States. doi:10.1016/J.VIROL.2016.04.004.
Niu, Yuhui, Zhang, Tingfu, Zhu, Ying, Yuan, Yongze, Wang, Shengqiang, Liu, Jing, and Liu, Deli, E-mail: ldl@mail.ccnu.edu.cn. 2016. "Isolation and characterization of a novel mycovirus from Penicillium digitatum". United States. doi:10.1016/J.VIROL.2016.04.004.
@article{osti_22581694,
title = {Isolation and characterization of a novel mycovirus from Penicillium digitatum},
author = {Niu, Yuhui and Zhang, Tingfu and Zhu, Ying and Yuan, Yongze and Wang, Shengqiang and Liu, Jing and Liu, Deli, E-mail: ldl@mail.ccnu.edu.cn},
abstractNote = {A novel double-stranded RNA virus designated Penicillium digitatum virus 1 (PdV1) was isolated from the citrus fruit rot pathogen P. digitatum (HS-RH1). The full-length cDNA sequence of the dsRNA/PdV1 (5211 bp) possesses two partially overlapping open reading frames, which encode a coat protein (CP) and a putative RNA-dependent RNA polymerase (RdRp), respectively. Phylogenetic analysis based on multiple alignments of the amino acid sequences of the RdRp and CP indicated that PdV1 tentatively belongs to the genus Victorivirus in the Totiviridae family. Electron micrographs of negatively stained viral particles purified from the peak fraction of sucrose density gradient centrifugation showed spherical particles ~35 nm in diameter. Transfection experiments with purified virions indicated that PdV1 could reduce the vegetative growth and virulence of P. digitatum strain HS-F6. In summary, we report the first isolation and characterization of a mycovirus from P. digitatum that contributes to the hypovirulence phenotypes of the host strain. - Highlights: • A novel victorivirus designated Penicillium digitatum virus 1 (PdV1) was isolated fromP. digitatum. • The dsRNA genome of PdV1 are 5211 bp long, two ORFs encoding CP and RdRp, and are encased in virions of ~35 nm in diameter. • PdV1 infection led to hypovirulent effect on P. digitatum. PdV1 may potentially be used for citrus green mold biocontrol. • Our study provides a research basis for establishing a model system for the study of P. digitatum–mycovirus interactions.},
doi = {10.1016/J.VIROL.2016.04.004},
journal = {Virology},
number = ,
volume = 494,
place = {United States},
year = 2016,
month = 7
}