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Title: Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection

Abstract

Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation. - Highlights: • Glycoprotein B (gB) is highly conserved among herpesviruses. • HHV-6 gB is also abundantly expressed in virions. • In the present study, we showed the function of HHV-6 gB cytoplasmic tail domain (CTD). • We found that deletion of gB CTD impairs the intracellular transport of gB protein to the trans-Golgi network (TGN), and CTD of gB is critical for HHV-6 propagation.

Authors:
 [1];  [2];  [1];  [3]; ; ; ;  [1];  [1];  [4];  [1];  [1];  [5];  [1]
  1. Division of Clinical Virology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, Kobe (Japan)
  2. (Egypt)
  3. (Indonesia)
  4. (Japan)
  5. (China)
Publication Date:
OSTI Identifier:
22581676
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 490; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GLYCOPROTEINS; REVERTANTS; VIRAL DISEASES; VIRUSES

Citation Formats

Mahmoud, Nora F., Faculty of Pharmacy, Suez Canal University, Ismailia, Jasirwan, Chyntia, Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Kanemoto, Satoshi, Wakata, Aika, Wang, Bochao, Hata, Yuuki, Nagamata, Satoshi, Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Kawabata, Akiko, Tang, Huamin, Department of Immunology, Nanjing Medical University, Nanjing, and Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.jp. Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection. United States: N. p., 2016. Web. doi:10.1016/J.VIROL.2015.12.018.
Mahmoud, Nora F., Faculty of Pharmacy, Suez Canal University, Ismailia, Jasirwan, Chyntia, Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Kanemoto, Satoshi, Wakata, Aika, Wang, Bochao, Hata, Yuuki, Nagamata, Satoshi, Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Kawabata, Akiko, Tang, Huamin, Department of Immunology, Nanjing Medical University, Nanjing, & Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.jp. Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection. United States. doi:10.1016/J.VIROL.2015.12.018.
Mahmoud, Nora F., Faculty of Pharmacy, Suez Canal University, Ismailia, Jasirwan, Chyntia, Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Kanemoto, Satoshi, Wakata, Aika, Wang, Bochao, Hata, Yuuki, Nagamata, Satoshi, Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe, Kawabata, Akiko, Tang, Huamin, Department of Immunology, Nanjing Medical University, Nanjing, and Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.jp. Tue . "Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection". United States. doi:10.1016/J.VIROL.2015.12.018.
@article{osti_22581676,
title = {Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection},
author = {Mahmoud, Nora F. and Faculty of Pharmacy, Suez Canal University, Ismailia and Jasirwan, Chyntia and Division of Hepatobiliary, Department of Internal Medicine, Faculty of Medicine, University of Indonesia and Kanemoto, Satoshi and Wakata, Aika and Wang, Bochao and Hata, Yuuki and Nagamata, Satoshi and Department of Obstetrics and Gynecology, Kobe University Graduate School of Medicine, Kobe and Kawabata, Akiko and Tang, Huamin and Department of Immunology, Nanjing Medical University, Nanjing and Mori, Yasuko, E-mail: ymori@med.kobe-u.ac.jp},
abstractNote = {Human herpesvirus 6 (HHV-6) glycoprotein B (gB) is an abundantly expressed viral glycoprotein required for viral entry and cell fusion, and is highly conserved among herpesviruses. The present study examined the function of HHV-6 gB cytoplasmic tail domain (CTD). A gB CTD deletion mutant was constructed which, in contrast to its revertant, could not be reconstituted. Moreover, deletion of gB cytoplasmic tail impaired the intracellular transport of gB protein to the trans-Golgi network (TGN). Taken together, these results suggest that gB CTD is critical for HHV-6 propagation and important for intracellular transportation. - Highlights: • Glycoprotein B (gB) is highly conserved among herpesviruses. • HHV-6 gB is also abundantly expressed in virions. • In the present study, we showed the function of HHV-6 gB cytoplasmic tail domain (CTD). • We found that deletion of gB CTD impairs the intracellular transport of gB protein to the trans-Golgi network (TGN), and CTD of gB is critical for HHV-6 propagation.},
doi = {10.1016/J.VIROL.2015.12.018},
journal = {Virology},
number = ,
volume = 490,
place = {United States},
year = {Tue Mar 15 00:00:00 EDT 2016},
month = {Tue Mar 15 00:00:00 EDT 2016}
}