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Title: Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development

Abstract

Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60%. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo up to the blastocyst stage after exposure of the embryos from different mice to a femtosecond pulse is presented. It is shown that the efficiency of blastocyst formation essentially depends on the biological characteristics of the embryo, namely, the strain and age of the donor mouse. The possibility of obtaining hexaploid embryonal cells using the methods of femtosecond laser surgery is demonstrated. (extreme light fields and their applications)

Authors:
; ; ; ;  [1];  [2]
  1. N N Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow (Russian Federation)
  2. A M Prokhorov General Physics Institute, Russian Academy of Sciences, Moscow (Russian Federation)
Publication Date:
OSTI Identifier:
22551264
Resource Type:
Journal Article
Journal Name:
Quantum Electronics (Woodbury, N.Y.)
Additional Journal Information:
Journal Volume: 45; Journal Issue: 5; Other Information: Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1063-7818
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; EFFICIENCY; EMBRYOS; LASER RADIATION; MICE; PULSES; STRAINS; SURGERY

Citation Formats

Osychenko, A A, Zalesskii, A D, Krivokharchenko, A S, Zhakhbazyan, A K, Nadtochenko, V A, and Ryabova, A V. Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development. United States: N. p., 2015. Web. doi:10.1070/QE2015V045N05ABEH015767.
Osychenko, A A, Zalesskii, A D, Krivokharchenko, A S, Zhakhbazyan, A K, Nadtochenko, V A, & Ryabova, A V. Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development. United States. doi:10.1070/QE2015V045N05ABEH015767.
Osychenko, A A, Zalesskii, A D, Krivokharchenko, A S, Zhakhbazyan, A K, Nadtochenko, V A, and Ryabova, A V. Sun . "Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development". United States. doi:10.1070/QE2015V045N05ABEH015767.
@article{osti_22551264,
title = {Fusion of blastomeres in mouse embryos under the action of femtosecond laser radiation. Efficiency of blastocyst formation and embryo development},
author = {Osychenko, A A and Zalesskii, A D and Krivokharchenko, A S and Zhakhbazyan, A K and Nadtochenko, V A and Ryabova, A V},
abstractNote = {Using the method of femtosecond laser surgery we study the fusion of two-cell mouse embryos under the action of tightly focused femtosecond laser radiation with the fusion efficiency reaching 60%. The detailed statistical analysis of the efficiency of blastomere fusion and development of the embryo up to the blastocyst stage after exposure of the embryos from different mice to a femtosecond pulse is presented. It is shown that the efficiency of blastocyst formation essentially depends on the biological characteristics of the embryo, namely, the strain and age of the donor mouse. The possibility of obtaining hexaploid embryonal cells using the methods of femtosecond laser surgery is demonstrated. (extreme light fields and their applications)},
doi = {10.1070/QE2015V045N05ABEH015767},
journal = {Quantum Electronics (Woodbury, N.Y.)},
issn = {1063-7818},
number = 5,
volume = 45,
place = {United States},
year = {2015},
month = {5}
}