Native sulfur/chlorine SAD phasing for serial femtosecond crystallography

Journal Article · Fri Nov 27 00:00:00 EST 2015 · Acta Crystallographica. Section D: Biological Crystallography

DOI:https://doi.org/10.1107/S139900471501857X· OSTI ID:22515199

Nakane, Takanori ^[1]; Song, Changyong ^[2]; POSTECH, Pohang 790-784 ^[3]; Suzuki, Mamoru ^[2]; Nango, Eriko; Kobayashi, Jun ^[2]; Masuda, Tetsuya ^[2]; Inoue, Shigeyuki ^[2]; Mizohata, Eiichi ^[4]; Nakatsu, Toru ^[2]; Tanaka, Tomoyuki; Tanaka, Rie ^[2]; Shimamura, Tatsuro ^[5]; Tono, Kensuke; Joti, Yasumasa; Kameshima, Takashi ^[6]; Hatsui, Takaki; Yabashi, Makina ^[2]; Nureki, Osamu ^[1]; Iwata, So ^[2] ; Sugahara, Michihiro ^[2]

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1. The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan)
2. RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148 (Japan)
3. Korea, Republic of
4. Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871 (Japan)
5. Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501 (Japan)
6. Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198 (Japan)

Sulfur SAD phasing facilitates the structure determination of diverse native proteins using femtosecond X-rays from free-electron lasers via serial femtosecond crystallography. Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.

• OSTI ID: 22515199
• Journal Information: Acta Crystallographica. Section D: Biological Crystallography, Vol. 71, Issue Pt 12; Other Information: PMCID: PMC4667287; PMID: 26627659; PUBLISHER-ID: wa5097; OAI: oai:pubmedcentral.nih.gov:4667287; Copyright (c) Nakane et al. 2015; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); ISSN 0907-4449
• Country of Publication: Denmark
• Language: English

Related Subjects

75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTAL STRUCTURE
CRYSTALLOGRAPHY
FREE ELECTRON LASERS
RADIATION EFFECTS
SULFUR
X RADIATION