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Title: Protein stability: a crystallographer’s perspective

Abstract

An understanding of protein stability is essential for optimizing the expression, purification and crystallization of proteins. In this review, discussion will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer. Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.

Authors:
 [1];  [2];  [3];  [4]
  1. Stanford University, Shriram Center, 443 Via Ortega, Room 097, MC5082, Stanford, CA 94305-4125 (United States)
  2. National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Building 8, Room 1A03, 8 Center Drive, Bethesda, MD 20814 (United States)
  3. k.-k. Hofkristallamt, 91 Audrey Place, Vista, CA 92084 (United States)
  4. (Austria)
Publication Date:
OSTI Identifier:
22515179
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F, Structural Biology Communications
Additional Journal Information:
Journal Volume: 72; Journal Issue: Pt 2; Other Information: PMCID: PMC4741188; PMID: 26841758; PUBLISHER-ID: en5571; PUBLISHER-ID: S2053230X15024619; OAI: oai:pubmedcentral.nih.gov:4741188; Copyright (c) Deller et al. 2016; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 2053-230X
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; PROTEINS; STABILITY

Citation Formats

Deller, Marc C., E-mail: mdeller@stanford.edu, Kong, Leopold, Rupp, Bernhard, and Medical University of Innsbruck, Schöpfstrasse 41, A-6020 Innsbruck. Protein stability: a crystallographer’s perspective. United States: N. p., 2016. Web. doi:10.1107/S2053230X15024619.
Deller, Marc C., E-mail: mdeller@stanford.edu, Kong, Leopold, Rupp, Bernhard, & Medical University of Innsbruck, Schöpfstrasse 41, A-6020 Innsbruck. Protein stability: a crystallographer’s perspective. United States. doi:10.1107/S2053230X15024619.
Deller, Marc C., E-mail: mdeller@stanford.edu, Kong, Leopold, Rupp, Bernhard, and Medical University of Innsbruck, Schöpfstrasse 41, A-6020 Innsbruck. Tue . "Protein stability: a crystallographer’s perspective". United States. doi:10.1107/S2053230X15024619.
@article{osti_22515179,
title = {Protein stability: a crystallographer’s perspective},
author = {Deller, Marc C., E-mail: mdeller@stanford.edu and Kong, Leopold and Rupp, Bernhard and Medical University of Innsbruck, Schöpfstrasse 41, A-6020 Innsbruck},
abstractNote = {An understanding of protein stability is essential for optimizing the expression, purification and crystallization of proteins. In this review, discussion will focus on factors affecting protein stability on a somewhat practical level, particularly from the view of a protein crystallographer. Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.},
doi = {10.1107/S2053230X15024619},
journal = {Acta Crystallographica. Section F, Structural Biology Communications},
issn = {2053-230X},
number = Pt 2,
volume = 72,
place = {United States},
year = {2016},
month = {1}
}