Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns

Ramamoorthy, Sripriya; Zhang, Yuan; Jacques, Steven; Petrie, Tracy; Wang, Ruikang; Nuttall, Alfred L.

doi:10.1063/1.4939345

Title: Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns

Full Record
Other Related Research

Abstract

In this study, we have developed a phase-sensitive Fourier-domain optical coherence tomography system to simultaneously measure the in vivo inner ear vibrations in the hook area and second turn of the mouse cochlea. This technical development will enable measurement of intra-cochlear distortion products at ideal locations such as the distortion product generation site and reflection site. This information is necessary to un-mix the complex mixture of intra-cochlear waves comprising the DPOAE and thus leads to the non-invasive identification of the local region of cochlear damage.

Authors:

Ramamoorthy, Sripriya ^[1]; Zhang, Yuan; Jacques, Steven ^[2]; Petrie, Tracy; Wang, Ruikang ^[3]; Nuttall, Alfred L. ^[1]

Oregon Hearing Research Center, Oregon Health & Science University, Portland, Oregon (United States)
Department of Biomedical Engineering, Oregon Health & Science University, Portland, Oregon (United States)
Department of Bioengineering, University of Washington, Seattle, Washington (United States)

Publication Date:: Thu Dec 31 00:00:00 EST 2015

OSTI Identifier:: 22494508

Resource Type:: Journal Article

Journal Name:: AIP Conference Proceedings

Additional Journal Information:: Journal Volume: 1703; Journal Issue: 1; Conference: 12. international workshop on the mechanics of hearing, Cape Sounio (Greece), 23-29 Jun 2014; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0094-243X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; AUDITORY ORGANS; DAMAGE; IN VIVO; MICE; OPTICAL EQUIPMENT; REFLECTION; TOMOGRAPHY

Citation Formats


                    Ramamoorthy, Sripriya, Zhang, Yuan, Jacques, Steven, Petrie, Tracy, Wang, Ruikang, Nuttall, Alfred L., and Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan. Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns.  United States: N. p., 2015. 
        Web.  doi:10.1063/1.4939345.

Copy to clipboard


                    Ramamoorthy, Sripriya, Zhang, Yuan, Jacques, Steven, Petrie, Tracy, Wang, Ruikang, Nuttall, Alfred L., & Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan. Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns.  United States.  https://doi.org/10.1063/1.4939345

Copy to clipboard


                    Ramamoorthy, Sripriya, Zhang, Yuan, Jacques, Steven, Petrie, Tracy, Wang, Ruikang, Nuttall, Alfred L., and Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan. 2015.  
        "Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns".  United States.  https://doi.org/10.1063/1.4939345.

Copy to clipboard


                    
@article{osti_22494508,

  title        = {Development of a phase-sensitive Fourier domain optical coherence tomography system to measure mouse organ of Corti vibrations in two cochlear turns},

  author       = {Ramamoorthy, Sripriya and Zhang, Yuan and Jacques, Steven and Petrie, Tracy and Wang, Ruikang and Nuttall, Alfred L. and Kresge Hearing Research Institute, The University of Michigan, Ann Arbor, Michigan},

  abstractNote = {In this study, we have developed a phase-sensitive Fourier-domain optical coherence tomography system to simultaneously measure the in vivo inner ear vibrations in the hook area and second turn of the mouse cochlea. This technical development will enable measurement of intra-cochlear distortion products at ideal locations such as the distortion product generation site and reflection site. This information is necessary to un-mix the complex mixture of intra-cochlear waves comprising the DPOAE and thus leads to the non-invasive identification of the local region of cochlear damage.},

  doi          = {10.1063/1.4939345},

  url          = {https://www.osti.gov/biblio/22494508},
  journal      = {AIP Conference Proceedings},

  issn         = {0094-243X},

  number       = 1,

  volume       = 1703,

  place        = {United States},

  year         = {Thu Dec 31 00:00:00 EST 2015},

  month        = {Thu Dec 31 00:00:00 EST 2015}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1063/1.4939345

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Swept source optical coherence tomography for in vivo imaging and vibrometry in the apex of the mouse cochlea

Journal Article Lee, Hee; Raphael, Patrick; Oghalai, John; ... - AIP Conference Proceedings

Cochlear amplification has been most commonly investigated by measuring the vibrations of the basilar membrane in animal models. Several different techniques have been used for measuring these vibrations such as laser Doppler vibrometry, miniature pressure sensors, low coherence interferometry, and spectral-domain optical coherence tomography (SD-OCT). We have built a swept-source OCT (SS-OCT) system, which is similar to SD-OCT in that it is capable of performing both imaging and vibration measurements within the mouse cochlea in vivo without having to open the bone. In vivo 3D images of a mouse cochlea were obtained, and the basilar membrane, tectorial membrane, Reissner’s membrane,more »« less
https://doi.org/10.1063/1.4939344
Gain and frequency tuning within the mouse cochlear apex

Journal Article Oghalai, John; Raphael, Patrick; Gao, Simon; ... - AIP Conference Proceedings

Normal mammalian hearing requires cochlear outer hair cell active processes that amplify the traveling wave with high gain and sharp tuning, termed cochlear amplification. We have used optical coherence tomography to study cochlear amplification within the apical turn of the mouse cochlea. We measured not only classical basilar membrane vibratory tuning curves but also vibratory responses from the rest of the tissues that compose the organ of Corti. Basilar membrane tuning was sharp in live mice and broad in dead mice, whereas other regions of the organ of Corti demonstrated phase shifts consistent with additional filtering beyond that provided bymore »« less
https://doi.org/10.1063/1.4939339
CLRN1 Is Nonessential in the Mouse Retina but Is Required for Cochlear Hair Cell Development

Journal Article Geller, Scott; Guerin, Karen; Visel, Meike; ... - PLoS Genetics

Mutations in the CLRN1 gene cause Usher syndrome type 3 (USH3), a human disease characterized by progressive blindness and deafness. Clarin 1, the protein product of CLRN1, is a four-transmembrane protein predicted to be associated with ribbon synapses of photoreceptors and cochlear hair cells, and recently demonstrated to be associated with the cytoskeleton. To study Clrn1, we created a Clrn1 knockout (KO) mouse and characterized the histological and functional consequences of Clrn1 deletion in the retina and cochlea. Clrn1 KO mice do not develop a retinal degeneration phenotype, but exhibit progressive loss of sensory hair cells in the cochlea andmore »« less
https://doi.org/10.1371/journal.pgen.1000607

Full Text Available
Cloning and characterization of the mouse homolog (D12H14S564E) of a novel human cochlear gene (D14S564E)

Journal Article Kovatch, K; Robertson, N; Brody, T - American Journal of Human Genetics

We have isolated mouse cDNA clones homologous to a novel human cochlear cDNA (D14S564E) identified by subtractive hybridization of a human fetal cochlear cDNA library with human fetal brain RNA. The human gene is preferentially expressed in cochlea, with low level expression in brain and eye, and has three transcripts of 2.0, 2.3, and 2.9 kb. To investigate the structure and function of the human cochlear gene and its role in the biology of hearing and deafness, we have cloned the mouse homolog (D12H14S564E). Mouse cDNAs, ranging from 2.0 to 3.5 kb, were identified with a mouse brain cDNA librarymore »« less
Quantitative X-ray Tomography of the Mouse Cochlea

Journal Article Rau, Christoph; Hwang, Margaret; Lee, Wah-Keat; ... - PLoS ONE

Imaging with hard X-rays allows visualizing cochlear structures while maintaining intrinsic qualities of the tissue, including structure and size. With coherent X-rays, soft tissues, including membranes, can be imaged as well as cells making use of the so-called in-line phase contrast. In the present experiments, partially coherent synchrotron radiation has been used for micro-tomography. Three-dimensional reconstructions of the mouse cochlea have been created using the EM3D software and the volume has been segmented in the Amira Software Suite. The structures that have been reconstructed include scala tympani, scala media, scala vestibuli, Reissner’s membrane, basilar membrane, tectorial membrane, organ of Corti,more »« less
https://doi.org/10.1371/journal.pone.0033568

Full Text Available

Similar Records