skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Maternal hemochromatosis gene H63D single-nucleotide polymorphism and lead levels of placental tissue, maternal and umbilical cord blood

Abstract

Human hemochromatosis protein (HFE), a major histocompatibility complex class I-like integral membrane protein, participates in the down regulation of intestinal iron absorption by binding to transferrin receptor (TR). HFE competes with transferrin-bound iron for the TR and thus reduces uptake of iron into cells. On the other hand, a lack of HFE increases the intestinal absorption of iron similarly to iron deficiency associated with increasing in absorption and deposition of lead. During pregnancy, placenta cannot prevent transfer lead to the fetus; even low-level lead poisoning causes neurodevelopmental toxicity in children. The aim of this study was to determine the association between the maternal HFE H63D single-nucleotide polymorphism and lead levels in placental tissue, maternal blood and umbilical cord bloods. The study population comprised 93 mother–placenta pairs. Venous blood from mother was collected to investigate lead levels and HFE polymorphism that was detected by standard PCR–RFLP technique. Cord bloods and placentas were collected for lead levels which were analyzed by dual atomic absorption spectrometer system. The HFE H63D genotype frequencies of mothers were found as 75.3% homozygote typical (HH), 23.6% heterozygote (HD) and 1.1% homozygote atypical (DD). Our study results showed that the placental tissue, umbilical cord and maternal blood leadmore » levels of mothers with HD+DD genotypes were significantly higher than those with HH genotype (p<0.05). The present study indicated for the first time that mothers with H63D gene variants have higher lead levels of their newborn's placentas and umbilical cord bloods. - Highlights: • Mothers with H63D gene variants have higher lead levels of their newborn's umbilical cord blood. • Unborn child of women with HD+DD genotypes may be at increased risk of internal exposure to lead. • Maternal HFE status may have an effect on increased placenta, maternal and cord blood lead levels. • Maternal HFE status may have an effect on lead transfer from maternal to fetal circulation. • Placental, maternal and cord blood lead levels were not correlated with mothers' age.« less

Authors:
 [1];  [1];  [1];  [1]
  1. Ankara University, Institute of Forensic Sciences, Ankara (Turkey)
Publication Date:
OSTI Identifier:
22483307
Resource Type:
Journal Article
Journal Name:
Environmental Research
Additional Journal Information:
Journal Volume: 140; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0013-9351
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; BLOOD; GENOTYPE; HISTOCOMPATIBILITY COMPLEX; HUMAN POPULATIONS; INFANTS; INTESTINAL ABSORPTION; IRON; LEAD; MOTHS; NEONATES; NUCLEOTIDES; PLACENTA; PLANT TISSUES; POLYMERASE CHAIN REACTION; RECEPTORS; TRANSFERRIN

Citation Formats

Kayaalti, Zeliha, Kaya-Akyüzlü, Dilek, Söylemez, Esma, Middle Black Sea Passage Generation of Agricultural Research Station Director, Tokat, and Söylemezoğlu, Tülin. Maternal hemochromatosis gene H63D single-nucleotide polymorphism and lead levels of placental tissue, maternal and umbilical cord blood. United States: N. p., 2015. Web. doi:10.1016/J.ENVRES.2015.05.004.
Kayaalti, Zeliha, Kaya-Akyüzlü, Dilek, Söylemez, Esma, Middle Black Sea Passage Generation of Agricultural Research Station Director, Tokat, & Söylemezoğlu, Tülin. Maternal hemochromatosis gene H63D single-nucleotide polymorphism and lead levels of placental tissue, maternal and umbilical cord blood. United States. https://doi.org/10.1016/J.ENVRES.2015.05.004
Kayaalti, Zeliha, Kaya-Akyüzlü, Dilek, Söylemez, Esma, Middle Black Sea Passage Generation of Agricultural Research Station Director, Tokat, and Söylemezoğlu, Tülin. 2015. "Maternal hemochromatosis gene H63D single-nucleotide polymorphism and lead levels of placental tissue, maternal and umbilical cord blood". United States. https://doi.org/10.1016/J.ENVRES.2015.05.004.
@article{osti_22483307,
title = {Maternal hemochromatosis gene H63D single-nucleotide polymorphism and lead levels of placental tissue, maternal and umbilical cord blood},
author = {Kayaalti, Zeliha and Kaya-Akyüzlü, Dilek and Söylemez, Esma and Middle Black Sea Passage Generation of Agricultural Research Station Director, Tokat and Söylemezoğlu, Tülin},
abstractNote = {Human hemochromatosis protein (HFE), a major histocompatibility complex class I-like integral membrane protein, participates in the down regulation of intestinal iron absorption by binding to transferrin receptor (TR). HFE competes with transferrin-bound iron for the TR and thus reduces uptake of iron into cells. On the other hand, a lack of HFE increases the intestinal absorption of iron similarly to iron deficiency associated with increasing in absorption and deposition of lead. During pregnancy, placenta cannot prevent transfer lead to the fetus; even low-level lead poisoning causes neurodevelopmental toxicity in children. The aim of this study was to determine the association between the maternal HFE H63D single-nucleotide polymorphism and lead levels in placental tissue, maternal blood and umbilical cord bloods. The study population comprised 93 mother–placenta pairs. Venous blood from mother was collected to investigate lead levels and HFE polymorphism that was detected by standard PCR–RFLP technique. Cord bloods and placentas were collected for lead levels which were analyzed by dual atomic absorption spectrometer system. The HFE H63D genotype frequencies of mothers were found as 75.3% homozygote typical (HH), 23.6% heterozygote (HD) and 1.1% homozygote atypical (DD). Our study results showed that the placental tissue, umbilical cord and maternal blood lead levels of mothers with HD+DD genotypes were significantly higher than those with HH genotype (p<0.05). The present study indicated for the first time that mothers with H63D gene variants have higher lead levels of their newborn's placentas and umbilical cord bloods. - Highlights: • Mothers with H63D gene variants have higher lead levels of their newborn's umbilical cord blood. • Unborn child of women with HD+DD genotypes may be at increased risk of internal exposure to lead. • Maternal HFE status may have an effect on increased placenta, maternal and cord blood lead levels. • Maternal HFE status may have an effect on lead transfer from maternal to fetal circulation. • Placental, maternal and cord blood lead levels were not correlated with mothers' age.},
doi = {10.1016/J.ENVRES.2015.05.004},
url = {https://www.osti.gov/biblio/22483307}, journal = {Environmental Research},
issn = {0013-9351},
number = ,
volume = 140,
place = {United States},
year = {Wed Jul 15 00:00:00 EDT 2015},
month = {Wed Jul 15 00:00:00 EDT 2015}
}