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Title: Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging

Abstract

We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.

Authors:
; ; ; ; ; ;  [1]; ; ;  [2]
  1. Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Mail Stop G755, Los Alamos, New Mexico 87545 (United States)
  2. Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131 (United States)
Publication Date:
OSTI Identifier:
22482678
Resource Type:
Journal Article
Resource Relation:
Journal Name: Review of Scientific Instruments; Journal Volume: 86; Journal Issue: 12; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; CAMERAS; CORRELATIONS; FLUORESCENCE; LIFETIME; MICROSCOPES; MOLECULES; NOISE; PHOTONS; PROTEINS; QUANTUM DOTS; RAMAN EFFECT; SIGNALS; SPECTROSCOPY; TIME RESOLUTION

Citation Formats

DeVore, M. S., Stich, D. G., Keller, A. M., Phipps, M. E., Hollingsworth, J. A., Goodwin, P. M., Werner, J. H., E-mail: jwerner@lanl.gov, Cleyrat, C., Lidke, D. S., and Wilson, B. S. Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging. United States: N. p., 2015. Web. doi:10.1063/1.4937477.
DeVore, M. S., Stich, D. G., Keller, A. M., Phipps, M. E., Hollingsworth, J. A., Goodwin, P. M., Werner, J. H., E-mail: jwerner@lanl.gov, Cleyrat, C., Lidke, D. S., & Wilson, B. S. Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging. United States. doi:10.1063/1.4937477.
DeVore, M. S., Stich, D. G., Keller, A. M., Phipps, M. E., Hollingsworth, J. A., Goodwin, P. M., Werner, J. H., E-mail: jwerner@lanl.gov, Cleyrat, C., Lidke, D. S., and Wilson, B. S. 2015. "Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging". United States. doi:10.1063/1.4937477.
@article{osti_22482678,
title = {Note: Time-gated 3D single quantum dot tracking with simultaneous spinning disk imaging},
author = {DeVore, M. S. and Stich, D. G. and Keller, A. M. and Phipps, M. E. and Hollingsworth, J. A. and Goodwin, P. M. and Werner, J. H., E-mail: jwerner@lanl.gov and Cleyrat, C. and Lidke, D. S. and Wilson, B. S.},
abstractNote = {We describe recent upgrades to a 3D tracking microscope to include simultaneous Nipkow spinning disk imaging and time-gated single-particle tracking (SPT). Simultaneous 3D molecular tracking and spinning disk imaging enable the visualization of cellular structures and proteins around a given fluorescently labeled target molecule. The addition of photon time-gating to the SPT hardware improves signal to noise by discriminating against Raman scattering and short-lived fluorescence. In contrast to camera-based SPT, single-photon arrival times are recorded, enabling time-resolved spectroscopy (e.g., measurement of fluorescence lifetimes and photon correlations) to be performed during single molecule/particle tracking experiments.},
doi = {10.1063/1.4937477},
journal = {Review of Scientific Instruments},
number = 12,
volume = 86,
place = {United States},
year = 2015,
month =
}
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