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Title: Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses

Abstract

Summary: We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses. - Highlights: • The complete genome of alfalfa dwarf virus is obtained. • An integrated localization and interaction map for ADV is determined. • ADV has a genome sequence similarity and evolutionary links with cytorhabdoviruses. • ADV protein localization and interaction data show an association with the nucleus. • ADV combinesmore » properties of both cytoplasmic and nuclear plant rhabdoviruses.« less

Authors:
 [1];  [2]; ; ; ; ;  [1];  [3]
  1. Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria INTA, Camino a 60 Cuadras k 5,5, Córdoba X5020ICA (Argentina)
  2. (Australia)
  3. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072 (Australia)
Publication Date:
OSTI Identifier:
22470189
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 483; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALFALFA; AMINO ACID SEQUENCE; FLUORESCENCE; INTERACTIONS; LEAVES; NICOTIANA; NUCLEOTIDES; ORIENTATION; PHOSPHOPROTEINS; RNA; TRANSIENTS; VEHICLES; VIRUSES

Citation Formats

Bejerman, Nicolás, E-mail: n.bejerman@uq.edu.au, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Giolitti, Fabián, Breuil, Soledad de, Trucco, Verónica, Nome, Claudia, Lenardon, Sergio, and Dietzgen, Ralf G. Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. United States: N. p., 2015. Web. doi:10.1016/J.VIROL.2015.05.001.
Bejerman, Nicolás, E-mail: n.bejerman@uq.edu.au, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Giolitti, Fabián, Breuil, Soledad de, Trucco, Verónica, Nome, Claudia, Lenardon, Sergio, & Dietzgen, Ralf G. Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. United States. doi:10.1016/J.VIROL.2015.05.001.
Bejerman, Nicolás, E-mail: n.bejerman@uq.edu.au, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Giolitti, Fabián, Breuil, Soledad de, Trucco, Verónica, Nome, Claudia, Lenardon, Sergio, and Dietzgen, Ralf G. Tue . "Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses". United States. doi:10.1016/J.VIROL.2015.05.001.
@article{osti_22470189,
title = {Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses},
author = {Bejerman, Nicolás, E-mail: n.bejerman@uq.edu.au and Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072 and Giolitti, Fabián and Breuil, Soledad de and Trucco, Verónica and Nome, Claudia and Lenardon, Sergio and Dietzgen, Ralf G.},
abstractNote = {Summary: We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses. - Highlights: • The complete genome of alfalfa dwarf virus is obtained. • An integrated localization and interaction map for ADV is determined. • ADV has a genome sequence similarity and evolutionary links with cytorhabdoviruses. • ADV protein localization and interaction data show an association with the nucleus. • ADV combines properties of both cytoplasmic and nuclear plant rhabdoviruses.},
doi = {10.1016/J.VIROL.2015.05.001},
journal = {Virology},
number = ,
volume = 483,
place = {United States},
year = {Tue Sep 15 00:00:00 EDT 2015},
month = {Tue Sep 15 00:00:00 EDT 2015}
}