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Title: Low-dose radiation pretreatment improves survival of human ceiling culture-derived proliferative adipocytes (ccdPAs) under hypoxia via HIF-1 alpha and MMP-2 induction

Abstract

Poor survival is a major problem of adipocyte transplantation. We previously reported that VEGF and MMPs secreted from transplanted adipocytes are essential for angiogenesis and adipogenesis. Pretreatment with low-dose (5 Gy) radiation (LDR) increased VEGF, MMP-2, and HIF-1 alpha mRNA expression in human ceiling culture-derived proliferative adipocytes (hccdPAs). Gene expression after LDR differed between adipose-derived stem cells (hASCs) and hccdPAs. Pretreatment with LDR improved the survival of hccdPAs under hypoxia, which is inevitable in the early stages after transplantation. Upregulation of VEGF and MMP-2 after LDR in hccdPAs is mediated by HIF-1 alpha expression. Our results suggest that pretreatment with LDR may improve adipocyte graft survival in a clinical setting through upregulation of VEGF and MMP-2 via HIF-1 alpha. - Highlights: • Ceiling culture-derived proliferative adipocytes (ccdPAs) react to radiation. • Low-dose radiation (LDR) pretreatment improves survival of ccdPAs under hypoxia. • Gene expression after LDR differs between ccdPAs and adipose-derived stem cells. • LDR-induced increase in MMP-2 and VEGF is dependent on HIF-1 alpha induction. • LDR pretreatment may improve the adipocyte graft survival rate in clinical settings.

Authors:
 [1];  [1]; ; ; ;  [1];  [2];  [1];  [3];  [1]
  1. Department of Plastic Surgery, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba-city, Chiba, #260-8677 (Japan)
  2. Center for Advanced Medicine, Chiba University, 1-8-1, Inohana, Chuo-ku, Chiba-city, Chiba, #260-8677 (Japan)
  3. Department of Clinical-Laboratory and Experimental-Research Medicine, Toho University, Sakura Medical Center, 564-1 Shimoshizu, Sakura-shi, Chiba, #285-8741 (Japan)
Publication Date:
OSTI Identifier:
22462166
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 463; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANGIOGENESIS; ANOXIA; CELL CULTURES; GENES; GRAFTS; MESSENGER-RNA; PROTEINS; RADIATION DOSES; STEM CELLS

Citation Formats

Adachi, Naoki, Kubota, Yoshitaka, Kosaka, Kentarou, Akita, Shinsuke, Sasahara, Yoshitarou, Kira, Tomoe, Kuroda, Masayuki, Mitsukawa, Nobuyuki, Bujo, Hideaki, and Satoh, Kaneshige. Low-dose radiation pretreatment improves survival of human ceiling culture-derived proliferative adipocytes (ccdPAs) under hypoxia via HIF-1 alpha and MMP-2 induction. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.06.079.
Adachi, Naoki, Kubota, Yoshitaka, Kosaka, Kentarou, Akita, Shinsuke, Sasahara, Yoshitarou, Kira, Tomoe, Kuroda, Masayuki, Mitsukawa, Nobuyuki, Bujo, Hideaki, & Satoh, Kaneshige. Low-dose radiation pretreatment improves survival of human ceiling culture-derived proliferative adipocytes (ccdPAs) under hypoxia via HIF-1 alpha and MMP-2 induction. United States. doi:10.1016/J.BBRC.2015.06.079.
Adachi, Naoki, Kubota, Yoshitaka, Kosaka, Kentarou, Akita, Shinsuke, Sasahara, Yoshitarou, Kira, Tomoe, Kuroda, Masayuki, Mitsukawa, Nobuyuki, Bujo, Hideaki, and Satoh, Kaneshige. Fri . "Low-dose radiation pretreatment improves survival of human ceiling culture-derived proliferative adipocytes (ccdPAs) under hypoxia via HIF-1 alpha and MMP-2 induction". United States. doi:10.1016/J.BBRC.2015.06.079.
@article{osti_22462166,
title = {Low-dose radiation pretreatment improves survival of human ceiling culture-derived proliferative adipocytes (ccdPAs) under hypoxia via HIF-1 alpha and MMP-2 induction},
author = {Adachi, Naoki and Kubota, Yoshitaka and Kosaka, Kentarou and Akita, Shinsuke and Sasahara, Yoshitarou and Kira, Tomoe and Kuroda, Masayuki and Mitsukawa, Nobuyuki and Bujo, Hideaki and Satoh, Kaneshige},
abstractNote = {Poor survival is a major problem of adipocyte transplantation. We previously reported that VEGF and MMPs secreted from transplanted adipocytes are essential for angiogenesis and adipogenesis. Pretreatment with low-dose (5 Gy) radiation (LDR) increased VEGF, MMP-2, and HIF-1 alpha mRNA expression in human ceiling culture-derived proliferative adipocytes (hccdPAs). Gene expression after LDR differed between adipose-derived stem cells (hASCs) and hccdPAs. Pretreatment with LDR improved the survival of hccdPAs under hypoxia, which is inevitable in the early stages after transplantation. Upregulation of VEGF and MMP-2 after LDR in hccdPAs is mediated by HIF-1 alpha expression. Our results suggest that pretreatment with LDR may improve adipocyte graft survival in a clinical setting through upregulation of VEGF and MMP-2 via HIF-1 alpha. - Highlights: • Ceiling culture-derived proliferative adipocytes (ccdPAs) react to radiation. • Low-dose radiation (LDR) pretreatment improves survival of ccdPAs under hypoxia. • Gene expression after LDR differs between ccdPAs and adipose-derived stem cells. • LDR-induced increase in MMP-2 and VEGF is dependent on HIF-1 alpha induction. • LDR pretreatment may improve the adipocyte graft survival rate in clinical settings.},
doi = {10.1016/J.BBRC.2015.06.079},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 463,
place = {United States},
year = {2015},
month = {8}
}