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Title: Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells

Abstract

Stromal-derived Factor-1 (SDF-1) derived from vascular smooth muscle cells (VSMCs) contributes to vascular repair and remodeling in various vascular diseases. In this study, the mechanism underlying regulation of SDF-1 expression by interleukin-1α (IL-1α) was investigated in primary rat VSMCs. We found IL-1α promotes SDF-1 expression by up-regulating CCAAT-enhancer-binding protein β (C/EBPβ) in an IκB kinase β (IKKβ) signaling-dependent manner. Moreover, IL-1α-induced expression of C/EBPβ and SDF-1 was significantly potentiated by knockdown of transforming growth factor β-activated kinase 1 (TAK1), an upstream activator of IKKβ signaling. In addition, we also demonstrated that TAK1/p38 mitogen-activated protein kinase (p38 MAPK) signaling exerted negative effect on IL-1α-induced expression of C/EBPβ and SDF-1 through counteracting ROS-dependent up-regulation of nuclear factor erythroid 2-related factor 2 (NRF2). In conclusion, TAK1 acts as an important regulator of IL-1α-induced SDF-1 expression in VSMCs, and modulating activity of TAK1 may serve as a potential strategy for modulating vascular repair and remodeling. - Highlights: • IL-1α induces IKKβ signaling-dependent SDF-1 expression by up-regulating C/EBPβ. • Activation of TAK1 by IL-1α negatively regulates C/EBPβ-dependent SDF-1 expression. • IL-1α-induced TAK1/p38 MAPK signaling counteracts ROS-dependent SDF-1 expression. • TAK1 counteracts IL-1α-induced SDF-1 expression by attenuating NRF2 up-regulation.

Authors:
 [1];  [2];  [1];  [3]; ; ; ; ; ; ; ; ;  [1];  [4];  [1]
  1. Department of Hepatobiliary Surgery, Union Hospital, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430022 (China)
  2. Department of Gerontology, Union Hospital, Tongji Medical College, Huangzhong University of Science and Technology, Wuhan 430022 (China)
  3. Department of Hepatobiliary Pancreatic Surgery, People's Hospital of Zhengzhou University, School of Medicine, Zhengzhou University, Zhengzhou 450003 (China)
  4. Department of Hepatobiliary Surgery, The Second Hospital, Hebei Medical University, Shijiazhuang 050000 (China)
Publication Date:
OSTI Identifier:
22462115
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 463; Journal Issue: 1-2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL REPAIR; CYSTEINE; GROWTH FACTORS; MUSCLES; PHOSPHOTRANSFERASES; RATS; SIGNALS; VASCULAR DISEASES

Citation Formats

Yang, Bin, Li, Wei, Zheng, Qichang, Qin, Tao, Wang, Kun, Li, Jinjin, Guo, Bing, Yu, Qihong, Wu, Yuzhe, Gao, Yang, Cheng, Xiang, Hu, Shaobo, Kumar, Stanley Naveen, Liu, Sanguang, E-mail: sanguang1998@sina.com, and Song, Zifang, E-mail: zsong@hust.edu.cn. Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.05.047.
Yang, Bin, Li, Wei, Zheng, Qichang, Qin, Tao, Wang, Kun, Li, Jinjin, Guo, Bing, Yu, Qihong, Wu, Yuzhe, Gao, Yang, Cheng, Xiang, Hu, Shaobo, Kumar, Stanley Naveen, Liu, Sanguang, E-mail: sanguang1998@sina.com, & Song, Zifang, E-mail: zsong@hust.edu.cn. Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells. United States. doi:10.1016/J.BBRC.2015.05.047.
Yang, Bin, Li, Wei, Zheng, Qichang, Qin, Tao, Wang, Kun, Li, Jinjin, Guo, Bing, Yu, Qihong, Wu, Yuzhe, Gao, Yang, Cheng, Xiang, Hu, Shaobo, Kumar, Stanley Naveen, Liu, Sanguang, E-mail: sanguang1998@sina.com, and Song, Zifang, E-mail: zsong@hust.edu.cn. Fri . "Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells". United States. doi:10.1016/J.BBRC.2015.05.047.
@article{osti_22462115,
title = {Transforming growth factor β-activated kinase 1 negatively regulates interleukin-1α-induced stromal-derived factor-1 expression in vascular smooth muscle cells},
author = {Yang, Bin and Li, Wei and Zheng, Qichang and Qin, Tao and Wang, Kun and Li, Jinjin and Guo, Bing and Yu, Qihong and Wu, Yuzhe and Gao, Yang and Cheng, Xiang and Hu, Shaobo and Kumar, Stanley Naveen and Liu, Sanguang, E-mail: sanguang1998@sina.com and Song, Zifang, E-mail: zsong@hust.edu.cn},
abstractNote = {Stromal-derived Factor-1 (SDF-1) derived from vascular smooth muscle cells (VSMCs) contributes to vascular repair and remodeling in various vascular diseases. In this study, the mechanism underlying regulation of SDF-1 expression by interleukin-1α (IL-1α) was investigated in primary rat VSMCs. We found IL-1α promotes SDF-1 expression by up-regulating CCAAT-enhancer-binding protein β (C/EBPβ) in an IκB kinase β (IKKβ) signaling-dependent manner. Moreover, IL-1α-induced expression of C/EBPβ and SDF-1 was significantly potentiated by knockdown of transforming growth factor β-activated kinase 1 (TAK1), an upstream activator of IKKβ signaling. In addition, we also demonstrated that TAK1/p38 mitogen-activated protein kinase (p38 MAPK) signaling exerted negative effect on IL-1α-induced expression of C/EBPβ and SDF-1 through counteracting ROS-dependent up-regulation of nuclear factor erythroid 2-related factor 2 (NRF2). In conclusion, TAK1 acts as an important regulator of IL-1α-induced SDF-1 expression in VSMCs, and modulating activity of TAK1 may serve as a potential strategy for modulating vascular repair and remodeling. - Highlights: • IL-1α induces IKKβ signaling-dependent SDF-1 expression by up-regulating C/EBPβ. • Activation of TAK1 by IL-1α negatively regulates C/EBPβ-dependent SDF-1 expression. • IL-1α-induced TAK1/p38 MAPK signaling counteracts ROS-dependent SDF-1 expression. • TAK1 counteracts IL-1α-induced SDF-1 expression by attenuating NRF2 up-regulation.},
doi = {10.1016/J.BBRC.2015.05.047},
journal = {Biochemical and Biophysical Research Communications},
number = 1-2,
volume = 463,
place = {United States},
year = {Fri Jul 17 00:00:00 EDT 2015},
month = {Fri Jul 17 00:00:00 EDT 2015}
}