skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Micropillar displacements by cell traction forces are mechanically correlated with nuclear dynamics

Abstract

Cells sense physical cues at the level of focal adhesions and transduce them to the nucleus by biochemical and mechanical pathways. While the molecular intermediates in the mechanical links have been well studied, their dynamic coupling is poorly understood. In this study, fibroblast cells were adhered to micropillar arrays to probe correlations in the physical coupling between focal adhesions and nucleus. For this, we used novel imaging setup to simultaneously visualize micropillar deflections and EGFP labeled chromatin structure at high spatial and temporal resolution. We observed that micropillar deflections, depending on their relative positions, were positively or negatively correlated to nuclear and heterochromatin movements. Our results measuring the time scales between micropillar deflections and nucleus centroid displacement are suggestive of a strong elastic coupling that mediates differential force transmission to the nucleus. - Highlights: • Correlation between focal adhesions and nucleus studied using novel imaging setup. • Micropillar and nuclear displacements were measured at high resolution. • Correlation timescales show strong elastic coupling between cell edge and nucleus.

Authors:
; ;  [1];  [1];  [2]
  1. Mechanobiology Institute, National University of Singapore (Singapore)
  2. (Singapore)
Publication Date:
OSTI Identifier:
22462064
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 461; Journal Issue: 2; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADHESION; BIOMEDICAL RADIOGRAPHY; FIBROBLASTS; HETEROCHROMATIN; RESOLUTION; TRANSMISSION

Citation Formats

Li, Qingsen, Makhija, Ekta, Hameed, F.M., Shivashankar, G.V., E-mail: shiva.gvs@gmail.com, and Department of Biological Sciences, National University of Singapore. Micropillar displacements by cell traction forces are mechanically correlated with nuclear dynamics. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.04.041.
Li, Qingsen, Makhija, Ekta, Hameed, F.M., Shivashankar, G.V., E-mail: shiva.gvs@gmail.com, & Department of Biological Sciences, National University of Singapore. Micropillar displacements by cell traction forces are mechanically correlated with nuclear dynamics. United States. doi:10.1016/J.BBRC.2015.04.041.
Li, Qingsen, Makhija, Ekta, Hameed, F.M., Shivashankar, G.V., E-mail: shiva.gvs@gmail.com, and Department of Biological Sciences, National University of Singapore. Fri . "Micropillar displacements by cell traction forces are mechanically correlated with nuclear dynamics". United States. doi:10.1016/J.BBRC.2015.04.041.
@article{osti_22462064,
title = {Micropillar displacements by cell traction forces are mechanically correlated with nuclear dynamics},
author = {Li, Qingsen and Makhija, Ekta and Hameed, F.M. and Shivashankar, G.V., E-mail: shiva.gvs@gmail.com and Department of Biological Sciences, National University of Singapore},
abstractNote = {Cells sense physical cues at the level of focal adhesions and transduce them to the nucleus by biochemical and mechanical pathways. While the molecular intermediates in the mechanical links have been well studied, their dynamic coupling is poorly understood. In this study, fibroblast cells were adhered to micropillar arrays to probe correlations in the physical coupling between focal adhesions and nucleus. For this, we used novel imaging setup to simultaneously visualize micropillar deflections and EGFP labeled chromatin structure at high spatial and temporal resolution. We observed that micropillar deflections, depending on their relative positions, were positively or negatively correlated to nuclear and heterochromatin movements. Our results measuring the time scales between micropillar deflections and nucleus centroid displacement are suggestive of a strong elastic coupling that mediates differential force transmission to the nucleus. - Highlights: • Correlation between focal adhesions and nucleus studied using novel imaging setup. • Micropillar and nuclear displacements were measured at high resolution. • Correlation timescales show strong elastic coupling between cell edge and nucleus.},
doi = {10.1016/J.BBRC.2015.04.041},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 461,
place = {United States},
year = {2015},
month = {5}
}