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Title: Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9

Abstract

We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, released at 10-fold higher levels, produced upon activation, including sublytic C5b-9. From electron microscopy, nanosight tracking analysis, dynamic light scattering and flow cytometry, cMVs (194–210 nm in diameter) are smaller than sMVs (333–385 nm). Furthermore, using a Quartz Crystal Microbalance measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, an sMV and a cMV are estimated at 0.267 and 0.241 pg, respectively. sMVs carry more calcium and protein, express higher levels of lipid rafts, GPI-anchored CD55 and phosphatidylserine including deposited C5b-9 compared to cMVs. This may allude to biological differences such as increased bound C4BP on sMVs inhibiting complement more effectively. - Highlights: • Prostate cells release microvesicles constitutively (cMVs) or upon stimulus (sMVs). • sMVs are larger than cMVs and carry more protein, lipid rafts and surface PstSer. • sMVs inhibit complement more effectively than cMVs.

Authors:
; ;  [1];  [2];  [1]
  1. Cellular and Molecular Immunology Research Centre, London Metropolitan University (United Kingdom)
  2. University College London School of Pharmacy, London UK (United Kingdom)
Publication Date:
OSTI Identifier:
22462034
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 460; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CALCIUM; COMPARATIVE EVALUATIONS; COMPLEMENT; DEPOSITS; INHIBITION; LIGHT SCATTERING; LIPIDS; METABOLISM; MICROBALANCES; NEOPLASMS; PROSTATE; QUARTZ; SENSORS; STIMULATION; SURFACES

Citation Formats

Stratton, Dan, Moore, Colin, Antwi-Baffour, Samuel, Lange, Sigrun, and Inal, Jameel, E-mail: j.inal@londonmet.ac.uk. Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.03.074.
Stratton, Dan, Moore, Colin, Antwi-Baffour, Samuel, Lange, Sigrun, & Inal, Jameel, E-mail: j.inal@londonmet.ac.uk. Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. United States. doi:10.1016/J.BBRC.2015.03.074.
Stratton, Dan, Moore, Colin, Antwi-Baffour, Samuel, Lange, Sigrun, and Inal, Jameel, E-mail: j.inal@londonmet.ac.uk. Fri . "Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9". United States. doi:10.1016/J.BBRC.2015.03.074.
@article{osti_22462034,
title = {Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9},
author = {Stratton, Dan and Moore, Colin and Antwi-Baffour, Samuel and Lange, Sigrun and Inal, Jameel, E-mail: j.inal@londonmet.ac.uk},
abstractNote = {We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, released at 10-fold higher levels, produced upon activation, including sublytic C5b-9. From electron microscopy, nanosight tracking analysis, dynamic light scattering and flow cytometry, cMVs (194–210 nm in diameter) are smaller than sMVs (333–385 nm). Furthermore, using a Quartz Crystal Microbalance measuring changes in resonant frequency (Δf) that equate to mass deposited on a sensor, an sMV and a cMV are estimated at 0.267 and 0.241 pg, respectively. sMVs carry more calcium and protein, express higher levels of lipid rafts, GPI-anchored CD55 and phosphatidylserine including deposited C5b-9 compared to cMVs. This may allude to biological differences such as increased bound C4BP on sMVs inhibiting complement more effectively. - Highlights: • Prostate cells release microvesicles constitutively (cMVs) or upon stimulus (sMVs). • sMVs are larger than cMVs and carry more protein, lipid rafts and surface PstSer. • sMVs inhibit complement more effectively than cMVs.},
doi = {10.1016/J.BBRC.2015.03.074},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 3,
volume = 460,
place = {United States},
year = {2015},
month = {5}
}