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Title: miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma

Abstract

microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3′ untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC. - Highlights: • miR-204-5p expression is downregulated in PTC tissues and cell lines. • miR-204-5p suppresses proliferation and promotes apoptosis in PTC cells. • miR-204-5p suppresses IGFBP5 expression by direct binding to the 3′-UTR. • IGFBP5 overexpression reverses the effects of miR-204-5p.

Authors:
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Publication Date:
OSTI Identifier:
22458492
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 457; Journal Issue: 4; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; CARCINOMAS; CELL CYCLE; CELL PROLIFERATION; GROWTH FACTORS; INSULIN; LUCIFERASE; MESSENGER-RNA; PLANT GROWTH; THYROID

Citation Formats

Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, and Gu, Mingjun, E-mail: mjgugonglihos@yeah.net. miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2015.01.037.
Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, & Gu, Mingjun, E-mail: mjgugonglihos@yeah.net. miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma. United States. doi:10.1016/J.BBRC.2015.01.037.
Liu, Lianyong, Wang, Jingnan, Li, Xiangqi, Ma, Junhua, Shi, Chao, Zhu, Hongling, Xi, Qian, Zhang, Jichen, Zhao, Xuemei, and Gu, Mingjun, E-mail: mjgugonglihos@yeah.net. Fri . "miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma". United States. doi:10.1016/J.BBRC.2015.01.037.
@article{osti_22458492,
title = {miR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma},
author = {Liu, Lianyong and Wang, Jingnan and Li, Xiangqi and Ma, Junhua and Shi, Chao and Zhu, Hongling and Xi, Qian and Zhang, Jichen and Zhao, Xuemei and Gu, Mingjun, E-mail: mjgugonglihos@yeah.net},
abstractNote = {microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3′ untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC. - Highlights: • miR-204-5p expression is downregulated in PTC tissues and cell lines. • miR-204-5p suppresses proliferation and promotes apoptosis in PTC cells. • miR-204-5p suppresses IGFBP5 expression by direct binding to the 3′-UTR. • IGFBP5 overexpression reverses the effects of miR-204-5p.},
doi = {10.1016/J.BBRC.2015.01.037},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 457,
place = {United States},
year = {Fri Feb 20 00:00:00 EST 2015},
month = {Fri Feb 20 00:00:00 EST 2015}
}