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Title: miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression

Abstract

A large proportion of the NSCLC patients were insensitive to radiotherapy, but the exact mechanism is still unclear. This study explored the role of miR-25 in regulating sensitivity of NSCLC cells to ionizing radiation (IR) and its downstream targets. Based on measurement in tumor samples from NSCLC patients, this study found that miR-25 expression is upregulated in both NSCLC and radio-resistant NSCLC patients compared the healthy and radio-sensitive controls. In addition, BTG expression was found negatively correlated with miR-25a expression in the both tissues and cells. By applying luciferase reporter assay, we verified two putative binding sites between miR-25 and BTG2. Therefore, BTG2 is a directly target of miR-25 in NSCLC cancer. By applying loss-and-gain function analysis in NSCLC cell lines, we demonstrated that miR-25-BTG2 axis could directly regulated BTG2 expression and affect radiotherapy sensitivity of NSCLC cells. - Highlights: • miR-25 is upregulated, while BTG2 is downregulated in radioresistant NSCLC patients. • miR-25 modulates sensitivity to radiation induced apoptosis. • miR-25 directly targets BTG2 and suppresses its expression. • miR-25 modulates sensitivity to radiotherapy through inhibiting BTG2 expression.

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22458470
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 457; Journal Issue: 3; Other Information: Copyright (c) 2015 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; APOPTOSIS; COMPARATIVE EVALUATIONS; LUCIFERASE; NEOPLASMS; PATIENTS; RADIOSENSITIVITY; RADIOTHERAPY

Citation Formats

He, Zhiwei, E-mail: carlhe@126.com, Liu, Yi, E-mail: cassieliu@126.com, Xiao, Bing, E-mail: rockg714@aliyun.com, and Qian, Xiaosen, E-mail: xiaosenqian@126.com. miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2014.12.094.
He, Zhiwei, E-mail: carlhe@126.com, Liu, Yi, E-mail: cassieliu@126.com, Xiao, Bing, E-mail: rockg714@aliyun.com, & Qian, Xiaosen, E-mail: xiaosenqian@126.com. miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression. United States. doi:10.1016/J.BBRC.2014.12.094.
He, Zhiwei, E-mail: carlhe@126.com, Liu, Yi, E-mail: cassieliu@126.com, Xiao, Bing, E-mail: rockg714@aliyun.com, and Qian, Xiaosen, E-mail: xiaosenqian@126.com. Fri . "miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression". United States. doi:10.1016/J.BBRC.2014.12.094.
@article{osti_22458470,
title = {miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression},
author = {He, Zhiwei, E-mail: carlhe@126.com and Liu, Yi, E-mail: cassieliu@126.com and Xiao, Bing, E-mail: rockg714@aliyun.com and Qian, Xiaosen, E-mail: xiaosenqian@126.com},
abstractNote = {A large proportion of the NSCLC patients were insensitive to radiotherapy, but the exact mechanism is still unclear. This study explored the role of miR-25 in regulating sensitivity of NSCLC cells to ionizing radiation (IR) and its downstream targets. Based on measurement in tumor samples from NSCLC patients, this study found that miR-25 expression is upregulated in both NSCLC and radio-resistant NSCLC patients compared the healthy and radio-sensitive controls. In addition, BTG expression was found negatively correlated with miR-25a expression in the both tissues and cells. By applying luciferase reporter assay, we verified two putative binding sites between miR-25 and BTG2. Therefore, BTG2 is a directly target of miR-25 in NSCLC cancer. By applying loss-and-gain function analysis in NSCLC cell lines, we demonstrated that miR-25-BTG2 axis could directly regulated BTG2 expression and affect radiotherapy sensitivity of NSCLC cells. - Highlights: • miR-25 is upregulated, while BTG2 is downregulated in radioresistant NSCLC patients. • miR-25 modulates sensitivity to radiation induced apoptosis. • miR-25 directly targets BTG2 and suppresses its expression. • miR-25 modulates sensitivity to radiotherapy through inhibiting BTG2 expression.},
doi = {10.1016/J.BBRC.2014.12.094},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 457,
place = {United States},
year = {Fri Feb 13 00:00:00 EST 2015},
month = {Fri Feb 13 00:00:00 EST 2015}
}