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Title: miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1

Abstract

Highlights: • miR-320 plays a significant role in chemoresistance. • This role might be attribute to targeting FOXM1. • The Wnt/β-catenin pathway also involves in this chemotherapy sensitivity. - Abstract: miR-320 expression level is found to be down-regulated in human colon cancer. To date, however, its underlying mechanisms in the chemo-resistance remain largely unknown. In this study, we demonstrated that ectopic expression of miR-320 led to inhibit HCT-116 cell proliferation, invasion and hypersensitivity to 5-Fu and Oxaliplatin. Also, knockdown of miR-320 reversed these effects in HT-29 cells. Furthermore, we identified an oncogene, FOXM1, as a direct target of miR-320. In addition, miR-320 could inactive the activity of Wnt/β-catenin pathway. Finally, we found that miR-320 and FOXM1 protein had a negative correlation in colon cancer tissues and adjacent normal tissues. These findings implied that miR-320–FOXM1 axis may overcome chemo-resistance of colon cancer cells and provide a new therapeutic target for the treatment of colon cancer.

Authors:
; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22458463
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 457; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CHEMOTHERAPY; COMBINED THERAPY; HUMAN POPULATIONS; IN VITRO; LARGE INTESTINE; MAMMARY GLANDS; NEOPLASMS; ONCOGENES; PLASMINOGEN; POLYMERASE CHAIN REACTION; RECEPTORS; SENSITIVITY; TOXINS; UROKINASE; X RADIATION

Citation Formats

Wan, Lu-Ying, Deng, Jun, Xiang, Xiao-Jun, Zhang, Ling, Yu, Feng, Chen, Jun, Sun, Zhe, Feng, Miao, and Xiong, Jian-Ping, E-mail: jpxiong@medmail.com.cn. miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2014.11.039.
Wan, Lu-Ying, Deng, Jun, Xiang, Xiao-Jun, Zhang, Ling, Yu, Feng, Chen, Jun, Sun, Zhe, Feng, Miao, & Xiong, Jian-Ping, E-mail: jpxiong@medmail.com.cn. miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1. United States. doi:10.1016/J.BBRC.2014.11.039.
Wan, Lu-Ying, Deng, Jun, Xiang, Xiao-Jun, Zhang, Ling, Yu, Feng, Chen, Jun, Sun, Zhe, Feng, Miao, and Xiong, Jian-Ping, E-mail: jpxiong@medmail.com.cn. Fri . "miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1". United States. doi:10.1016/J.BBRC.2014.11.039.
@article{osti_22458463,
title = {miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1},
author = {Wan, Lu-Ying and Deng, Jun and Xiang, Xiao-Jun and Zhang, Ling and Yu, Feng and Chen, Jun and Sun, Zhe and Feng, Miao and Xiong, Jian-Ping, E-mail: jpxiong@medmail.com.cn},
abstractNote = {Highlights: • miR-320 plays a significant role in chemoresistance. • This role might be attribute to targeting FOXM1. • The Wnt/β-catenin pathway also involves in this chemotherapy sensitivity. - Abstract: miR-320 expression level is found to be down-regulated in human colon cancer. To date, however, its underlying mechanisms in the chemo-resistance remain largely unknown. In this study, we demonstrated that ectopic expression of miR-320 led to inhibit HCT-116 cell proliferation, invasion and hypersensitivity to 5-Fu and Oxaliplatin. Also, knockdown of miR-320 reversed these effects in HT-29 cells. Furthermore, we identified an oncogene, FOXM1, as a direct target of miR-320. In addition, miR-320 could inactive the activity of Wnt/β-catenin pathway. Finally, we found that miR-320 and FOXM1 protein had a negative correlation in colon cancer tissues and adjacent normal tissues. These findings implied that miR-320–FOXM1 axis may overcome chemo-resistance of colon cancer cells and provide a new therapeutic target for the treatment of colon cancer.},
doi = {10.1016/J.BBRC.2014.11.039},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 457,
place = {United States},
year = {Fri Feb 06 00:00:00 EST 2015},
month = {Fri Feb 06 00:00:00 EST 2015}
}