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Title: MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro

Abstract

Highlights: • miRNA-196b increases proliferation and blocks differentiation of progenitor cell. • miRNA-196b inhibits apoptosis and increases viability of cells lines. • Forced expression of miR-196b blocks the differentiation of THP1 induced by PMA. - Abstract: MicroRNA-196b (miR-196b) is frequently amplified and aberrantly overexpressed in acute leukemias. To investigate the role of miR-196b in acute leukemias, it has been observed that forced expression of this miRNA increases proliferation and inhibits apoptosis in human cell lines. More importantly, we show that this miRNA can significantly increase the colony-forming capacity of mouse normal bone marrow progenitor cells alone, as well as partially blocking the cells from differentiation. Taken together, our studies suggest that miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22458455
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 457; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL CELLS; APOPTOSIS; BONE MARROW; CELL DIFFERENTIATION; CELL PROLIFERATION; IN VITRO; LEUKEMIA; MICE; VIABILITY

Citation Formats

Cao, Donglin, E-mail: caodlgz@sina.com, Hu, Liangshan, Lei, Da, Fang, Xiaolin, Zhang, Zhihong, Wang, Ting, Lin, Maorui, Huang, Jiwei, Yang, Huawen, Zhou, Xuan, and Zhong, Limei. MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2014.11.085.
Cao, Donglin, E-mail: caodlgz@sina.com, Hu, Liangshan, Lei, Da, Fang, Xiaolin, Zhang, Zhihong, Wang, Ting, Lin, Maorui, Huang, Jiwei, Yang, Huawen, Zhou, Xuan, & Zhong, Limei. MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro. United States. doi:10.1016/J.BBRC.2014.11.085.
Cao, Donglin, E-mail: caodlgz@sina.com, Hu, Liangshan, Lei, Da, Fang, Xiaolin, Zhang, Zhihong, Wang, Ting, Lin, Maorui, Huang, Jiwei, Yang, Huawen, Zhou, Xuan, and Zhong, Limei. Fri . "MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro". United States. doi:10.1016/J.BBRC.2014.11.085.
@article{osti_22458455,
title = {MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro},
author = {Cao, Donglin, E-mail: caodlgz@sina.com and Hu, Liangshan and Lei, Da and Fang, Xiaolin and Zhang, Zhihong and Wang, Ting and Lin, Maorui and Huang, Jiwei and Yang, Huawen and Zhou, Xuan and Zhong, Limei},
abstractNote = {Highlights: • miRNA-196b increases proliferation and blocks differentiation of progenitor cell. • miRNA-196b inhibits apoptosis and increases viability of cells lines. • Forced expression of miR-196b blocks the differentiation of THP1 induced by PMA. - Abstract: MicroRNA-196b (miR-196b) is frequently amplified and aberrantly overexpressed in acute leukemias. To investigate the role of miR-196b in acute leukemias, it has been observed that forced expression of this miRNA increases proliferation and inhibits apoptosis in human cell lines. More importantly, we show that this miRNA can significantly increase the colony-forming capacity of mouse normal bone marrow progenitor cells alone, as well as partially blocking the cells from differentiation. Taken together, our studies suggest that miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation.},
doi = {10.1016/J.BBRC.2014.11.085},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 457,
place = {United States},
year = {Fri Jan 30 00:00:00 EST 2015},
month = {Fri Jan 30 00:00:00 EST 2015}
}