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Title: Morphological classification of bioaerosols from composting using scanning electron microscopy

Abstract

Highlights: • Bioaerosols were captured using the filter method. • Bioaerosols were analysed using scanning electron microscope. • Bioaerosols were classified on the basis of morphology. • Single small cells were found more frequently than aggregates and larger cells. • Smaller cells may disperse further than heavier aggregate structures. - Abstract: This research classifies the physical morphology (form and structure) of bioaerosols emitted from open windrow composting. Aggregation state, shape and size of the particles captured are reported alongside the implications for bioaerosol dispersal after release. Bioaerosol sampling took place at a composting facility using personal air filter samplers. Samples were analysed using scanning electron microscopy. Particles were released mainly as small (<1 μm) single, spherical cells, followed by larger (>1 μm) single cells, with aggregates occurring in smaller proportions. Most aggregates consisted of clusters of 2–3 particles as opposed to chains, and were <10 μm in size. No cells were attached to soil debris or wood particles. These small single cells or small aggregates are more likely to disperse further downwind from source, and cell viability may be reduced due to increased exposure to environmental factors.

Authors:
 [1];  [2]; ; ; ;  [1];  [1]
  1. Institute for Energy and Resource Technology, Environmental Science and Technology Department, School of Applied Sciences, Cranfield University, Building 40, Bedfordshire MK43 0AL (United Kingdom)
  2. (United Kingdom)
Publication Date:
OSTI Identifier:
22443571
Resource Type:
Journal Article
Resource Relation:
Journal Name: Waste Management; Journal Volume: 34; Journal Issue: 7; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; AEROSOLS; AGGLOMERATION; AIR FILTERS; CLASSIFICATION; COMPOSTING; EMISSION; MORPHOLOGY; PARTICLE SIZE; PARTICLES; SAMPLERS; SAMPLING; SCANNING ELECTRON MICROSCOPY

Citation Formats

Tamer Vestlund, A., FIRA International Ltd., Maxwell Road, Stevenage, Herts SG1 2EW, Al-Ashaab, R., Tyrrel, S.F., Longhurst, P.J., Pollard, S.J.T., and Drew, G.H., E-mail: g.h.drew@cranfield.ac.uk. Morphological classification of bioaerosols from composting using scanning electron microscopy. United States: N. p., 2014. Web. doi:10.1016/J.WASMAN.2014.01.021.
Tamer Vestlund, A., FIRA International Ltd., Maxwell Road, Stevenage, Herts SG1 2EW, Al-Ashaab, R., Tyrrel, S.F., Longhurst, P.J., Pollard, S.J.T., & Drew, G.H., E-mail: g.h.drew@cranfield.ac.uk. Morphological classification of bioaerosols from composting using scanning electron microscopy. United States. doi:10.1016/J.WASMAN.2014.01.021.
Tamer Vestlund, A., FIRA International Ltd., Maxwell Road, Stevenage, Herts SG1 2EW, Al-Ashaab, R., Tyrrel, S.F., Longhurst, P.J., Pollard, S.J.T., and Drew, G.H., E-mail: g.h.drew@cranfield.ac.uk. Tue . "Morphological classification of bioaerosols from composting using scanning electron microscopy". United States. doi:10.1016/J.WASMAN.2014.01.021.
@article{osti_22443571,
title = {Morphological classification of bioaerosols from composting using scanning electron microscopy},
author = {Tamer Vestlund, A. and FIRA International Ltd., Maxwell Road, Stevenage, Herts SG1 2EW and Al-Ashaab, R. and Tyrrel, S.F. and Longhurst, P.J. and Pollard, S.J.T. and Drew, G.H., E-mail: g.h.drew@cranfield.ac.uk},
abstractNote = {Highlights: • Bioaerosols were captured using the filter method. • Bioaerosols were analysed using scanning electron microscope. • Bioaerosols were classified on the basis of morphology. • Single small cells were found more frequently than aggregates and larger cells. • Smaller cells may disperse further than heavier aggregate structures. - Abstract: This research classifies the physical morphology (form and structure) of bioaerosols emitted from open windrow composting. Aggregation state, shape and size of the particles captured are reported alongside the implications for bioaerosol dispersal after release. Bioaerosol sampling took place at a composting facility using personal air filter samplers. Samples were analysed using scanning electron microscopy. Particles were released mainly as small (<1 μm) single, spherical cells, followed by larger (>1 μm) single cells, with aggregates occurring in smaller proportions. Most aggregates consisted of clusters of 2–3 particles as opposed to chains, and were <10 μm in size. No cells were attached to soil debris or wood particles. These small single cells or small aggregates are more likely to disperse further downwind from source, and cell viability may be reduced due to increased exposure to environmental factors.},
doi = {10.1016/J.WASMAN.2014.01.021},
journal = {Waste Management},
number = 7,
volume = 34,
place = {United States},
year = {Tue Jul 15 00:00:00 EDT 2014},
month = {Tue Jul 15 00:00:00 EDT 2014}
}