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Title: Methyl parathion inhibits the nuclear maturation, decreases the cytoplasmic quality in oocytes and alters the developmental potential of embryos of Swiss albino mice

Abstract

Methyl parathion (MP) is one of the most commonly used and extremely toxic organophosphorous group of pesticide. A large number of studies in the literature suggest that it has adverse effects on the male reproductive system. However, there is limited information about its toxicity to the female reproductive system. In the present study we report the toxic effects of methyl parathion on the female reproductive system using Swiss albino mice as the experimental model. The female mice were administered orally with 5, 10 and 20 mg/kg of MP. One week later, the mice were superovulated with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) to study the quality of the oocytes, spindle organization, developmental potential of early embryos and the DNA integrity in blastocysts. MP exposure resulted in a non-significant decrease in the number of primordial follicles and increased DNA damage in granulosa cells. Though MP did not have any effect on the ovulation it had a significant inhibitory effect on the nuclear maturity of oocytes which was associated with spindle deformity. In addition, the oocytes had higher cytoplasmic abnormalities with depleted glutathione level. Even though it did not have any effect on the fertilization and blastocyst ratemore » at lower doses, at 20 mg/kg MP it resulted in a significant decrease in blastocyst hatching, decrease in cell number and high DNA damage. While low body weight gain was observed in F1 generation from 5 mg/kg group, at higher dose, the body weight in F1 generation was marginally higher than control. Post-natal death in F1 generation was observed only in mice treated with 20 mg/kg MP. In conclusion, we report that MP has adverse effects on the oocyte quality, developmental potential of the embryo and reproductive outcome. - Highlights: • Methyl parathion induces severe cytoplasmic abnormalities in oocytes. • Inhibits nuclear maturation and spindle damage • Poor blastocyst quality and high DNA damage.« less

Authors:
; ;  [1]; ;  [2]; ;  [3];  [1];  [1]
  1. Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal 576 104 (India)
  2. Department of Anatomy, Kasturba Medical College, Manipal University, Manipal 576 104 (India)
  3. Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal 576 104 (India)
Publication Date:
OSTI Identifier:
22439832
Resource Type:
Journal Article
Journal Name:
Toxicology and Applied Pharmacology
Additional Journal Information:
Journal Volume: 279; Journal Issue: 3; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0041-008X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; DAMAGE; DNA; DNA DAMAGES; DOSES; EMBRYOS; FERTILIZATION; GLUTATHIONE; HATCHING; HCG; MICE; MITOCHONDRIA; OOCYTES; OVULATION; PARATHION; TOXICITY

Citation Formats

Nair, Ramya, Singh, Vikram Jeet, Salian, Sujith Raj, Kalthur, Sneha Guruprasad, D'Souza, Antony Sylvan, Shetty, Pallavi K., Mutalik, Srinivas, Kalthur, Guruprasad, E-mail: guru.kalthur@manipal.edu, and Adiga, Satish Kumar. Methyl parathion inhibits the nuclear maturation, decreases the cytoplasmic quality in oocytes and alters the developmental potential of embryos of Swiss albino mice. United States: N. p., 2014. Web. doi:10.1016/J.TAAP.2014.07.004.
Nair, Ramya, Singh, Vikram Jeet, Salian, Sujith Raj, Kalthur, Sneha Guruprasad, D'Souza, Antony Sylvan, Shetty, Pallavi K., Mutalik, Srinivas, Kalthur, Guruprasad, E-mail: guru.kalthur@manipal.edu, & Adiga, Satish Kumar. Methyl parathion inhibits the nuclear maturation, decreases the cytoplasmic quality in oocytes and alters the developmental potential of embryos of Swiss albino mice. United States. doi:10.1016/J.TAAP.2014.07.004.
Nair, Ramya, Singh, Vikram Jeet, Salian, Sujith Raj, Kalthur, Sneha Guruprasad, D'Souza, Antony Sylvan, Shetty, Pallavi K., Mutalik, Srinivas, Kalthur, Guruprasad, E-mail: guru.kalthur@manipal.edu, and Adiga, Satish Kumar. Mon . "Methyl parathion inhibits the nuclear maturation, decreases the cytoplasmic quality in oocytes and alters the developmental potential of embryos of Swiss albino mice". United States. doi:10.1016/J.TAAP.2014.07.004.
@article{osti_22439832,
title = {Methyl parathion inhibits the nuclear maturation, decreases the cytoplasmic quality in oocytes and alters the developmental potential of embryos of Swiss albino mice},
author = {Nair, Ramya and Singh, Vikram Jeet and Salian, Sujith Raj and Kalthur, Sneha Guruprasad and D'Souza, Antony Sylvan and Shetty, Pallavi K. and Mutalik, Srinivas and Kalthur, Guruprasad, E-mail: guru.kalthur@manipal.edu and Adiga, Satish Kumar},
abstractNote = {Methyl parathion (MP) is one of the most commonly used and extremely toxic organophosphorous group of pesticide. A large number of studies in the literature suggest that it has adverse effects on the male reproductive system. However, there is limited information about its toxicity to the female reproductive system. In the present study we report the toxic effects of methyl parathion on the female reproductive system using Swiss albino mice as the experimental model. The female mice were administered orally with 5, 10 and 20 mg/kg of MP. One week later, the mice were superovulated with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) to study the quality of the oocytes, spindle organization, developmental potential of early embryos and the DNA integrity in blastocysts. MP exposure resulted in a non-significant decrease in the number of primordial follicles and increased DNA damage in granulosa cells. Though MP did not have any effect on the ovulation it had a significant inhibitory effect on the nuclear maturity of oocytes which was associated with spindle deformity. In addition, the oocytes had higher cytoplasmic abnormalities with depleted glutathione level. Even though it did not have any effect on the fertilization and blastocyst rate at lower doses, at 20 mg/kg MP it resulted in a significant decrease in blastocyst hatching, decrease in cell number and high DNA damage. While low body weight gain was observed in F1 generation from 5 mg/kg group, at higher dose, the body weight in F1 generation was marginally higher than control. Post-natal death in F1 generation was observed only in mice treated with 20 mg/kg MP. In conclusion, we report that MP has adverse effects on the oocyte quality, developmental potential of the embryo and reproductive outcome. - Highlights: • Methyl parathion induces severe cytoplasmic abnormalities in oocytes. • Inhibits nuclear maturation and spindle damage • Poor blastocyst quality and high DNA damage.},
doi = {10.1016/J.TAAP.2014.07.004},
journal = {Toxicology and Applied Pharmacology},
issn = {0041-008X},
number = 3,
volume = 279,
place = {United States},
year = {2014},
month = {9}
}