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Title: The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion

Abstract

Mutations in UL24 of herpes simplex virus type 1 can lead to a syncytial phenotype. We hypothesized that UL24 affects the sub-cellular distribution of viral glycoproteins involved in fusion. In non-immortalized human foreskin fibroblasts (HFFs) we detected viral glycoproteins B (gB), gD, gH and gL present in extended blotches throughout the cytoplasm with limited nuclear membrane staining; however, in HFFs infected with a UL24-deficient virus (UL24X), staining for the viral glycoproteins appeared as long, thin streaks running across the cell. Interestingly, there was a decrease in co-localized staining of gB and gD with F-actin at late times in UL24X-infected HFFs. Treatment with chemical agents that perturbed the actin cytoskeleton hindered the formation of UL24X-induced syncytia in these cells. These data support a model whereby the UL24 syncytial phenotype results from a mislocalization of viral glycoproteins late in infection. - Highlights: • UL24 affects the sub-cellular distribution of viral glycoproteins required for fusion. • Sub-cellular distribution of viral glycoproteins varies in cell-type dependent manner. • Drugs targeting actin microfilaments affect formation of UL24-related syncytia in HFFs.

Authors:
; ;
Publication Date:
OSTI Identifier:
22436648
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 444; Journal Issue: 1-2; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACTIN; CYTOPLASM; DISTRIBUTION; FIBROBLASTS; GLYCOPROTEINS; HERPES SIMPLEX; MEMBRANES; MICROTUBULES; MUTATIONS; PHENOTYPE; VIRUSES

Citation Formats

Ben Abdeljelil, Nawel, Rochette, Pierre-Alexandre, and Pearson, Angela, E-mail: angela.pearson@iaf.inrs.ca. The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion. United States: N. p., 2013. Web. doi:10.1016/J.VIROL.2013.06.021.
Ben Abdeljelil, Nawel, Rochette, Pierre-Alexandre, & Pearson, Angela, E-mail: angela.pearson@iaf.inrs.ca. The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion. United States. doi:10.1016/J.VIROL.2013.06.021.
Ben Abdeljelil, Nawel, Rochette, Pierre-Alexandre, and Pearson, Angela, E-mail: angela.pearson@iaf.inrs.ca. Sun . "The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion". United States. doi:10.1016/J.VIROL.2013.06.021.
@article{osti_22436648,
title = {The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion},
author = {Ben Abdeljelil, Nawel and Rochette, Pierre-Alexandre and Pearson, Angela, E-mail: angela.pearson@iaf.inrs.ca},
abstractNote = {Mutations in UL24 of herpes simplex virus type 1 can lead to a syncytial phenotype. We hypothesized that UL24 affects the sub-cellular distribution of viral glycoproteins involved in fusion. In non-immortalized human foreskin fibroblasts (HFFs) we detected viral glycoproteins B (gB), gD, gH and gL present in extended blotches throughout the cytoplasm with limited nuclear membrane staining; however, in HFFs infected with a UL24-deficient virus (UL24X), staining for the viral glycoproteins appeared as long, thin streaks running across the cell. Interestingly, there was a decrease in co-localized staining of gB and gD with F-actin at late times in UL24X-infected HFFs. Treatment with chemical agents that perturbed the actin cytoskeleton hindered the formation of UL24X-induced syncytia in these cells. These data support a model whereby the UL24 syncytial phenotype results from a mislocalization of viral glycoproteins late in infection. - Highlights: • UL24 affects the sub-cellular distribution of viral glycoproteins required for fusion. • Sub-cellular distribution of viral glycoproteins varies in cell-type dependent manner. • Drugs targeting actin microfilaments affect formation of UL24-related syncytia in HFFs.},
doi = {10.1016/J.VIROL.2013.06.021},
journal = {Virology},
issn = {0042-6822},
number = 1-2,
volume = 444,
place = {United States},
year = {2013},
month = {9}
}