skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Sequestration of human cytomegalovirus by human renal and mammary epithelial cells

Abstract

Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and late antigens within 72 h of infection. During the first 24 h after infection, high titers of infectious virus were measured associated to the cells and in culture supernatants, independently of de novo synthesis of virus progeny. This phenomenon was not observed in HCMV-infected fibroblasts and suggested the sequestration and the release of HCMV by epithelial cells. This hypothesis was supported by confocal and electron microscopy analyses. The sequestration and progressive release of HCMV by kidney and mammary epithelial cells may play an important role in the excretion of the virus in urine and breast milk and may thereby contribute to HCMV transmission. - Highlights: • Primary renal and mammary epithelial cells are permissive to HCMV infection. • HCMV is sequestered by epithelial cells and this phenomenon does not require viral replication. • HCMV sequestration by epithelial cells is reduced by antibodies and IFN-γ.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [2];  [1];  [7]
  1. Institute for Medical Immunology, Université Libre de Bruxelles, Rue A. Bolland 8, B-6041 Charleroi (Belgium)
  2. Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven (Belgium)
  3. ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi (Belgium)
  4. Department of Obstetrics and Gynecology, Erasme Hospital, Route de Lennik 808, 1070 Brussels (Belgium)
  5. Laboratory of Molecular Parasitology, Institut de Biologie et Médecine Moléculaires, Université Libre de Bruxelles, Gosselies (Belgium)
  6. Pathology Department, U.Z. Leuven, Minderbroedersstraat 12, Leuven (Belgium)
  7. (Belgium)
Publication Date:
OSTI Identifier:
22435043
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 460-461; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; ANTIGENS; ELECTRON MICROSCOPY; EXCRETION; FIBROBLASTS; GLYCOPROTEINS; HUMAN POPULATIONS; HYPOTHESIS; INTERFERON; KIDNEYS; MAMMARY GLANDS; MILK; PROGENY; SYNTHESIS; TRANSMISSION; URINE; VIRUSES

Citation Formats

Twite, Nicolas, Andrei, Graciela, Kummert, Caroline, Donner, Catherine, Perez-Morga, David, De Vos, Rita, Snoeck, Robert, E-mail: Robert.Snoeck@Rega.kuleuven.be, Marchant, Arnaud, E-mail: arnaud.marchant@ulb.ac.be, and ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi. Sequestration of human cytomegalovirus by human renal and mammary epithelial cells. United States: N. p., 2014. Web. doi:10.1016/J.VIROL.2014.04.032.
Twite, Nicolas, Andrei, Graciela, Kummert, Caroline, Donner, Catherine, Perez-Morga, David, De Vos, Rita, Snoeck, Robert, E-mail: Robert.Snoeck@Rega.kuleuven.be, Marchant, Arnaud, E-mail: arnaud.marchant@ulb.ac.be, & ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi. Sequestration of human cytomegalovirus by human renal and mammary epithelial cells. United States. doi:10.1016/J.VIROL.2014.04.032.
Twite, Nicolas, Andrei, Graciela, Kummert, Caroline, Donner, Catherine, Perez-Morga, David, De Vos, Rita, Snoeck, Robert, E-mail: Robert.Snoeck@Rega.kuleuven.be, Marchant, Arnaud, E-mail: arnaud.marchant@ulb.ac.be, and ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi. Tue . "Sequestration of human cytomegalovirus by human renal and mammary epithelial cells". United States. doi:10.1016/J.VIROL.2014.04.032.
@article{osti_22435043,
title = {Sequestration of human cytomegalovirus by human renal and mammary epithelial cells},
author = {Twite, Nicolas and Andrei, Graciela and Kummert, Caroline and Donner, Catherine and Perez-Morga, David and De Vos, Rita and Snoeck, Robert, E-mail: Robert.Snoeck@Rega.kuleuven.be and Marchant, Arnaud, E-mail: arnaud.marchant@ulb.ac.be and ImmuneHealth, Rue A. Bolland 8, B-6041 Charleroi},
abstractNote = {Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and late antigens within 72 h of infection. During the first 24 h after infection, high titers of infectious virus were measured associated to the cells and in culture supernatants, independently of de novo synthesis of virus progeny. This phenomenon was not observed in HCMV-infected fibroblasts and suggested the sequestration and the release of HCMV by epithelial cells. This hypothesis was supported by confocal and electron microscopy analyses. The sequestration and progressive release of HCMV by kidney and mammary epithelial cells may play an important role in the excretion of the virus in urine and breast milk and may thereby contribute to HCMV transmission. - Highlights: • Primary renal and mammary epithelial cells are permissive to HCMV infection. • HCMV is sequestered by epithelial cells and this phenomenon does not require viral replication. • HCMV sequestration by epithelial cells is reduced by antibodies and IFN-γ.},
doi = {10.1016/J.VIROL.2014.04.032},
journal = {Virology},
number = ,
volume = 460-461,
place = {United States},
year = {Tue Jul 15 00:00:00 EDT 2014},
month = {Tue Jul 15 00:00:00 EDT 2014}
}