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Title: miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes

Abstract

A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.

Authors:
 [1];  [2];  [3];  [3]
  1. Biomedical Sciences Research Institute, University of Ulster, Coleraine, Co. Derry BT52 1SA (United Kingdom)
  2. (United Kingdom)
  3. Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL (United Kingdom)
Publication Date:
OSTI Identifier:
22435005
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 448; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BALANCES; CELL PROLIFERATION; HUMAN POPULATIONS; PROTEINS; VIRUSES

Citation Formats

McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, E-mail: d.patel@qub.ac.uk, and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes. United States: N. p., 2014. Web. doi:10.1016/J.VIROL.2013.10.014.
McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, E-mail: d.patel@qub.ac.uk, & McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes. United States. doi:10.1016/J.VIROL.2013.10.014.
McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk, Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL, Patel, Daksha, E-mail: d.patel@qub.ac.uk, and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk. Sun . "miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes". United States. doi:10.1016/J.VIROL.2013.10.014.
@article{osti_22435005,
title = {miR-24 and miR-205 expression is dependent on HPV onco-protein expression in keratinocytes},
author = {McKenna, Declan J., E-mail: dj.mckenna@ulster.ac.uk and Centre for Cancer Research and Cell Biology, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast BT9 7BL and Patel, Daksha, E-mail: d.patel@qub.ac.uk and McCance, Dennis J., E-mail: d.mccance@qub.ac.uk},
abstractNote = {A screen of microRNA (miRNA) expression following differentiation in human foreskin keratinocytes (HFKs) identified changes in several miRNAs, including miR-24 and miR-205. We investigated how expression of Human Papilloma Virus Type-16 (HPV16) onco-proteins E6 and E7 affected expression of miR-24 and miR-205 during proliferation and differentiation of HFKs. We show that the induction of both miR-24 and miR-205 observed during differentiation of HFKs is lost in HFKs expressing E6 and E7. We demonstrate that the effect on miR-205 is due to E7 activity, as miR-205 expression is dependent on pRb expression. Finally, we provide evidence that miR-24 effects in the cell may be due to targeting of cyclin dependent kinase inhibitor p27. In summary, these results indicate that expression of both miR-24 and miR-205 are impacted by E6 and/or E7 expression, which may be one mechanism by which HPV onco-proteins can disrupt the balance between proliferation and differentiation in keratinocytes. - Highlights: • miR-24 and miR-205 are induced during keratinocyte differentiation. • This induction is lost in keratinocytes expressing HPV onco-proteins E6 and E7. • miR-205 is dependent upon pRb expression. • miR-24 targets p27 in cycling keratinocytes.},
doi = {10.1016/J.VIROL.2013.10.014},
journal = {Virology},
number = ,
volume = 448,
place = {United States},
year = {Sun Jan 05 00:00:00 EST 2014},
month = {Sun Jan 05 00:00:00 EST 2014}
}