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Title: Identification and characterization of a novel adenovirus in the cloacal bursa of gulls

Abstract

Several viruses of the family of Adenoviridae are associated with disease in birds. Here we report the detection of a novel adenovirus in the cloacal bursa of herring gulls (Larus argentatus) and lesser black-backed gulls (Larus fuscus) that were found dead in the Netherlands in 2001. Histopathological analysis of the cloacal bursa revealed cytomegaly and karyomegaly with basophilic intranuclear inclusions typical for adenovirus infection. The presence of an adenovirus was confirmed by electron microscopy. By random PCR in combination with deep sequencing, sequences were detected that had the best hit with known adenoviruses. Phylogenetic analysis of complete coding sequences of the hexon, penton and polymerase genes indicates that this novel virus, tentatively named Gull adenovirus, belongs to the genus Aviadenovirus. The present study demonstrates that birds of the Laridae family are infected by family-specific adenoviruses that differ from known adenoviruses in other bird species. - Highlights: ► Lesions typical for adenovirus infection detected in cloacal bursa of dead gulls. ► Confirmation of adenovirus infection by electron microscopy and deep sequencing. ► Sequence analysis indicates that it is a novel adenovirus in the genus Aviadenovirus. ► The novel (Gull) adenovirus was detected in multiple organs of two species of gulls.

Authors:
; ;  [1];  [2];  [1];  [3]; ;  [1];  [4];  [1]
  1. Department of Viroscience, Erasmus Medical Centre, Dr. Molewaterplein 50, 3015 GE Rotterdam (Netherlands)
  2. Viroclinics Biosciences, Marconistraat 16, 3029 AK Rotterdam (Netherlands)
  3. Department of Pathology, Erasmus Medical Centre, Dr. Molewaterplein 50, 3015 GE Rotterdam (Netherlands)
  4. (Netherlands)
Publication Date:
OSTI Identifier:
22423731
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 440; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADENOVIRUS; BIRDS; DETECTION; DISEASES; ELECTRON MICROSCOPY; GENES; NETHERLANDS; ORGANS; POLYMERASE CHAIN REACTION; STRUCTURAL CHEMICAL ANALYSIS

Citation Formats

Bodewes, R., Bildt, M.W.G. van de, Schapendonk, C.M.E., Leeuwen, M. van, Boheemen, S. van, Jong, A.A.W. de, Osterhaus, A.D.M.E., Smits, S.L., Viroclinics Biosciences, Marconistraat 16, 3029 AK Rotterdam, and Kuiken, T., E-mail: t.Kuiken@erasmusmc.nl. Identification and characterization of a novel adenovirus in the cloacal bursa of gulls. United States: N. p., 2013. Web. doi:10.1016/J.VIROL.2013.02.011.
Bodewes, R., Bildt, M.W.G. van de, Schapendonk, C.M.E., Leeuwen, M. van, Boheemen, S. van, Jong, A.A.W. de, Osterhaus, A.D.M.E., Smits, S.L., Viroclinics Biosciences, Marconistraat 16, 3029 AK Rotterdam, & Kuiken, T., E-mail: t.Kuiken@erasmusmc.nl. Identification and characterization of a novel adenovirus in the cloacal bursa of gulls. United States. doi:10.1016/J.VIROL.2013.02.011.
Bodewes, R., Bildt, M.W.G. van de, Schapendonk, C.M.E., Leeuwen, M. van, Boheemen, S. van, Jong, A.A.W. de, Osterhaus, A.D.M.E., Smits, S.L., Viroclinics Biosciences, Marconistraat 16, 3029 AK Rotterdam, and Kuiken, T., E-mail: t.Kuiken@erasmusmc.nl. 2013. "Identification and characterization of a novel adenovirus in the cloacal bursa of gulls". United States. doi:10.1016/J.VIROL.2013.02.011.
@article{osti_22423731,
title = {Identification and characterization of a novel adenovirus in the cloacal bursa of gulls},
author = {Bodewes, R. and Bildt, M.W.G. van de and Schapendonk, C.M.E. and Leeuwen, M. van and Boheemen, S. van and Jong, A.A.W. de and Osterhaus, A.D.M.E. and Smits, S.L. and Viroclinics Biosciences, Marconistraat 16, 3029 AK Rotterdam and Kuiken, T., E-mail: t.Kuiken@erasmusmc.nl},
abstractNote = {Several viruses of the family of Adenoviridae are associated with disease in birds. Here we report the detection of a novel adenovirus in the cloacal bursa of herring gulls (Larus argentatus) and lesser black-backed gulls (Larus fuscus) that were found dead in the Netherlands in 2001. Histopathological analysis of the cloacal bursa revealed cytomegaly and karyomegaly with basophilic intranuclear inclusions typical for adenovirus infection. The presence of an adenovirus was confirmed by electron microscopy. By random PCR in combination with deep sequencing, sequences were detected that had the best hit with known adenoviruses. Phylogenetic analysis of complete coding sequences of the hexon, penton and polymerase genes indicates that this novel virus, tentatively named Gull adenovirus, belongs to the genus Aviadenovirus. The present study demonstrates that birds of the Laridae family are infected by family-specific adenoviruses that differ from known adenoviruses in other bird species. - Highlights: ► Lesions typical for adenovirus infection detected in cloacal bursa of dead gulls. ► Confirmation of adenovirus infection by electron microscopy and deep sequencing. ► Sequence analysis indicates that it is a novel adenovirus in the genus Aviadenovirus. ► The novel (Gull) adenovirus was detected in multiple organs of two species of gulls.},
doi = {10.1016/J.VIROL.2013.02.011},
journal = {Virology},
number = 1,
volume = 440,
place = {United States},
year = 2013,
month = 5
}
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