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Title: Human Papillomavirus (HPV) Infection in Squamous Cell Carcinomas Arising From the Oropharynx: Detection of HPV DNA and p16 Immunohistochemistry as Diagnostic and Prognostic Indicators—A Pilot Study

Abstract

Purpose: Human papillomavirus (HPV) 16 infection is associated with oropharyngeal carcinogenesis and is likely the cause of the reported increase in disease incidence. We evaluated the prevalence of HPV infection and the reliability of different diagnostic tools using primary tumor samples from a cohort of 50 patients. Methods and Materials: Formalin-fixed paraffin-embedded (FFPE) tumor samples were collected from all 50 consecutive primary oropharyngeal SCC patients who were enrolled in the study; fresh tumor samples were available in 22 cases. NucliSENS EasyQ HPVv1 was used for RNA, and Digene Hybrid Capture-2(HC2) was used for DNA detection. p16 Expression was evaluated by immunohistochemistry in FPPE specimens. Results: Based on the DNA detection assay on FFPE samples, the frequency of high-risk HPV infection was 32%. The agreement rate between HPV RNA and HPV DNA detection in fresh samples was 100%. The agreement rate between p16 immunohistochemistry (IHC) and the detection of HPV DNA in the FFPE samples was fair but not excellent (κ = 0.618). HPV DNA detection was highly significant, as measured by disease-specific survival and determined using a Wilcoxon test (P=.001). p16 IHC also exhibited a prognostic value but with a lower statistical significance (P=.0475). The detection of HPV DNA, but not p16more » IHC, was also significantly correlated with locoregional control (P=.0461). Conclusion: Diagnostic methods based on the detection of HPV nucleic acids appear to be more reliable and objective because they do not require reading by a trained histopathologist. Furthermore, the detection of HPV DNA exhibits an improved correlation with survival, and therefore appears definitely more reliable than p16 IHC for routine use in clinical practice.« less

Authors:
 [1];  [2];  [1]; ;  [3]; ;  [4];  [2];  [3];  [1];  [4]; ;  [1]; ;  [2];  [3];
  1. Institute of Otolaryngology, Università Cattolica del Sacro Cuore, Policlinico A. Gemelli, Roma (Italy)
  2. Institute of Microbiology, Università Cattolica del Sacro Cuore, Policlinico A. Gemelli, Roma (Italy)
  3. Institute of Histopathology, Università Cattolica del Sacro Cuore, Policlinico A. Gemelli, Roma (Italy)
  4. Institute of Radiotherapy, Università Cattolica del Sacro Cuore, Policlinico A. Gemelli, Roma (Italy)
Publication Date:
OSTI Identifier:
22420401
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 89; Journal Issue: 5; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOGENESIS; CARCINOMAS; CORRELATIONS; DIAGNOSTIC TECHNIQUES; DISEASE INCIDENCE; DNA; HAZARDS; PATIENTS; PHARYNX; RNA

Citation Formats

Bussu, Francesco, E-mail: francesco.bussu.md@gmail.com, Sali, Michela, Gallus, Roberto, Petrone, Gianluigi, Zannoni, Gian Franco, Autorino, Rosa, Dinapoli, Nicola, Santangelo, Rosaria, Vellone, Valerio Gaetano, Graziani, Cristina, Miccichè, Francesco, Almadori, Giovanni, Galli, Jacopo, Delogu, Giovanni, Sanguinetti, Maurizio, Rindi, Guido, and and others. Human Papillomavirus (HPV) Infection in Squamous Cell Carcinomas Arising From the Oropharynx: Detection of HPV DNA and p16 Immunohistochemistry as Diagnostic and Prognostic Indicators—A Pilot Study. United States: N. p., 2014. Web. doi:10.1016/J.IJROBP.2014.04.044.
Bussu, Francesco, E-mail: francesco.bussu.md@gmail.com, Sali, Michela, Gallus, Roberto, Petrone, Gianluigi, Zannoni, Gian Franco, Autorino, Rosa, Dinapoli, Nicola, Santangelo, Rosaria, Vellone, Valerio Gaetano, Graziani, Cristina, Miccichè, Francesco, Almadori, Giovanni, Galli, Jacopo, Delogu, Giovanni, Sanguinetti, Maurizio, Rindi, Guido, & and others. Human Papillomavirus (HPV) Infection in Squamous Cell Carcinomas Arising From the Oropharynx: Detection of HPV DNA and p16 Immunohistochemistry as Diagnostic and Prognostic Indicators—A Pilot Study. United States. doi:10.1016/J.IJROBP.2014.04.044.
Bussu, Francesco, E-mail: francesco.bussu.md@gmail.com, Sali, Michela, Gallus, Roberto, Petrone, Gianluigi, Zannoni, Gian Franco, Autorino, Rosa, Dinapoli, Nicola, Santangelo, Rosaria, Vellone, Valerio Gaetano, Graziani, Cristina, Miccichè, Francesco, Almadori, Giovanni, Galli, Jacopo, Delogu, Giovanni, Sanguinetti, Maurizio, Rindi, Guido, and and others. Fri . "Human Papillomavirus (HPV) Infection in Squamous Cell Carcinomas Arising From the Oropharynx: Detection of HPV DNA and p16 Immunohistochemistry as Diagnostic and Prognostic Indicators—A Pilot Study". United States. doi:10.1016/J.IJROBP.2014.04.044.
@article{osti_22420401,
title = {Human Papillomavirus (HPV) Infection in Squamous Cell Carcinomas Arising From the Oropharynx: Detection of HPV DNA and p16 Immunohistochemistry as Diagnostic and Prognostic Indicators—A Pilot Study},
author = {Bussu, Francesco, E-mail: francesco.bussu.md@gmail.com and Sali, Michela and Gallus, Roberto and Petrone, Gianluigi and Zannoni, Gian Franco and Autorino, Rosa and Dinapoli, Nicola and Santangelo, Rosaria and Vellone, Valerio Gaetano and Graziani, Cristina and Miccichè, Francesco and Almadori, Giovanni and Galli, Jacopo and Delogu, Giovanni and Sanguinetti, Maurizio and Rindi, Guido and and others},
abstractNote = {Purpose: Human papillomavirus (HPV) 16 infection is associated with oropharyngeal carcinogenesis and is likely the cause of the reported increase in disease incidence. We evaluated the prevalence of HPV infection and the reliability of different diagnostic tools using primary tumor samples from a cohort of 50 patients. Methods and Materials: Formalin-fixed paraffin-embedded (FFPE) tumor samples were collected from all 50 consecutive primary oropharyngeal SCC patients who were enrolled in the study; fresh tumor samples were available in 22 cases. NucliSENS EasyQ HPVv1 was used for RNA, and Digene Hybrid Capture-2(HC2) was used for DNA detection. p16 Expression was evaluated by immunohistochemistry in FPPE specimens. Results: Based on the DNA detection assay on FFPE samples, the frequency of high-risk HPV infection was 32%. The agreement rate between HPV RNA and HPV DNA detection in fresh samples was 100%. The agreement rate between p16 immunohistochemistry (IHC) and the detection of HPV DNA in the FFPE samples was fair but not excellent (κ = 0.618). HPV DNA detection was highly significant, as measured by disease-specific survival and determined using a Wilcoxon test (P=.001). p16 IHC also exhibited a prognostic value but with a lower statistical significance (P=.0475). The detection of HPV DNA, but not p16 IHC, was also significantly correlated with locoregional control (P=.0461). Conclusion: Diagnostic methods based on the detection of HPV nucleic acids appear to be more reliable and objective because they do not require reading by a trained histopathologist. Furthermore, the detection of HPV DNA exhibits an improved correlation with survival, and therefore appears definitely more reliable than p16 IHC for routine use in clinical practice.},
doi = {10.1016/J.IJROBP.2014.04.044},
journal = {International Journal of Radiation Oncology, Biology and Physics},
number = 5,
volume = 89,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 2014},
month = {Fri Aug 01 00:00:00 EDT 2014}
}