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Title: Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2

Abstract

Purpose: The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we examined the roles of the vertebrate H2A.Z isoforms in chromatin reorganization after the induction of DNA double-strand breaks (DSBs). Methods and Materials: To examine the dynamics of H2A.Z isoforms at damaged sites, we constructed GM0637 cells stably expressing each of the green fluorescent protein (GFP)-labeled H2A.Z isoforms, and performed fluorescence recovery after photobleaching (FRAP) analysis and inverted FRAP analysis in combination with microirradiation. Immunofluorescence staining using an anti-RAD51 antibody was performed to study the kinetics of RAD51 foci formation after 2-Gy irradiation of wild-type (WT), H2A.Z-1- and H2A.Z-2-deficient DT40 cells. Colony-forming assays were also performed to compare the survival rates of WT, H2A.Z-1-, and H2A.Z-2-deficient DT40 cells with control, and H2A.Z-1- and H2A.Z-2-depleted U2OS cells after irradiation. Results: FRAP analysis revealed that H2A.Z-2 was incorporated into damaged chromatin just after the induction of DSBs, whereas H2A.Z-1 remained essentially unchanged. Inverted FRAP analysis showed that H2A.Z-2 was released from damaged chromatin. These findings indicated that H2A.Z-2 wasmore » exchanged at DSB sites immediately after the induction of DSBs. RAD51 focus formation after ionizing irradiation was disturbed in H2A.Z-2-deficient DT40 cells but not in H2A.Z-1-deficient cells. The survival rate of H2A.Z-2-deficient cells after irradiation was lower than those of WT and H2A.Z-1- DT40 cells. Similar to DT40 cells, H2A.Z-2-depleted U2OS cells were also radiation-sensitive compared to control and H2A.Z-1-depleted cells. Conclusions: We found that vertebrate H2A.Z-2 is involved in the regulation of the DNA damage response at a very early stage, via the damaged chromatin reorganization required for RAD51 focus formation.« less

Authors:
 [1];  [2];  [2]; ; ; ;  [1];  [1];  [2];  [3]; ;  [4];  [5];  [6];  [7];  [8];  [1];  [2]
  1. Department of Cellular Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima (Japan)
  2. (Japan)
  3. Department of Biochemistry, Graduate School of Medical Sciences, Tohoku University, Sendai (Japan)
  4. Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Sendai (Japan)
  5. Department of Molecular Genetics, National Institute of Genetics and The Graduate University for Advanced Studies, Mishima (Japan)
  6. Laboratory of Chromatin Regulatory Network, Department of Mutagenesis, Radiation Biology Center, Kyoto University, Kyoto (Japan)
  7. Department of Cardiovascular Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima (Japan)
  8. Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima (Japan)
Publication Date:
OSTI Identifier:
22420360
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 89; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; BIOLOGICAL RECOVERY; CHROMATIN; FLUORESCENCE; IRRADIATION; PROTEINS; STRAND BREAKS; VERTEBRATES

Citation Formats

Nishibuchi, Ikuno, Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima, Suzuki, Hidekazu, Kinomura, Aiko, Sun, Jiying, Liu, Ning-Ang, Horikoshi, Yasunori, Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima, Shima, Hiroki, Kusakabe, Masayuki, Harata, Masahiko, Fukagawa, Tatsuo, Ikura, Tsuyoshi, Ishida, Takafumi, Nagata, Yasushi, Tashiro, Satoshi, E-mail: ktashiro@hiroshima-u.ac.jp, and Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima. Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2. United States: N. p., 2014. Web. doi:10.1016/J.IJROBP.2014.03.031.
Nishibuchi, Ikuno, Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima, Suzuki, Hidekazu, Kinomura, Aiko, Sun, Jiying, Liu, Ning-Ang, Horikoshi, Yasunori, Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima, Shima, Hiroki, Kusakabe, Masayuki, Harata, Masahiko, Fukagawa, Tatsuo, Ikura, Tsuyoshi, Ishida, Takafumi, Nagata, Yasushi, Tashiro, Satoshi, E-mail: ktashiro@hiroshima-u.ac.jp, & Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima. Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2. United States. doi:10.1016/J.IJROBP.2014.03.031.
Nishibuchi, Ikuno, Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima, Suzuki, Hidekazu, Kinomura, Aiko, Sun, Jiying, Liu, Ning-Ang, Horikoshi, Yasunori, Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima, Shima, Hiroki, Kusakabe, Masayuki, Harata, Masahiko, Fukagawa, Tatsuo, Ikura, Tsuyoshi, Ishida, Takafumi, Nagata, Yasushi, Tashiro, Satoshi, E-mail: ktashiro@hiroshima-u.ac.jp, and Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima. Tue . "Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2". United States. doi:10.1016/J.IJROBP.2014.03.031.
@article{osti_22420360,
title = {Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2},
author = {Nishibuchi, Ikuno and Department of Radiation Oncology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima and Department of Radiation Oncology, Hiroshima Prefectural Hospital, Hiroshima and Suzuki, Hidekazu and Kinomura, Aiko and Sun, Jiying and Liu, Ning-Ang and Horikoshi, Yasunori and Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima and Shima, Hiroki and Kusakabe, Masayuki and Harata, Masahiko and Fukagawa, Tatsuo and Ikura, Tsuyoshi and Ishida, Takafumi and Nagata, Yasushi and Tashiro, Satoshi, E-mail: ktashiro@hiroshima-u.ac.jp and Research Center for Mathematics of Chromatin Live Dynamics, Hiroshima University, Hiroshima},
abstractNote = {Purpose: The reorganization of damaged chromatin plays an important role in the regulation of the DNA damage response. A recent study revealed the presence of 2 vertebrate H2A.Z isoforms, H2A.Z-1 and H2A.Z-2. However, the roles of the vertebrate H2A.Z isoforms are still unclear. Thus, in this study we examined the roles of the vertebrate H2A.Z isoforms in chromatin reorganization after the induction of DNA double-strand breaks (DSBs). Methods and Materials: To examine the dynamics of H2A.Z isoforms at damaged sites, we constructed GM0637 cells stably expressing each of the green fluorescent protein (GFP)-labeled H2A.Z isoforms, and performed fluorescence recovery after photobleaching (FRAP) analysis and inverted FRAP analysis in combination with microirradiation. Immunofluorescence staining using an anti-RAD51 antibody was performed to study the kinetics of RAD51 foci formation after 2-Gy irradiation of wild-type (WT), H2A.Z-1- and H2A.Z-2-deficient DT40 cells. Colony-forming assays were also performed to compare the survival rates of WT, H2A.Z-1-, and H2A.Z-2-deficient DT40 cells with control, and H2A.Z-1- and H2A.Z-2-depleted U2OS cells after irradiation. Results: FRAP analysis revealed that H2A.Z-2 was incorporated into damaged chromatin just after the induction of DSBs, whereas H2A.Z-1 remained essentially unchanged. Inverted FRAP analysis showed that H2A.Z-2 was released from damaged chromatin. These findings indicated that H2A.Z-2 was exchanged at DSB sites immediately after the induction of DSBs. RAD51 focus formation after ionizing irradiation was disturbed in H2A.Z-2-deficient DT40 cells but not in H2A.Z-1-deficient cells. The survival rate of H2A.Z-2-deficient cells after irradiation was lower than those of WT and H2A.Z-1- DT40 cells. Similar to DT40 cells, H2A.Z-2-depleted U2OS cells were also radiation-sensitive compared to control and H2A.Z-1-depleted cells. Conclusions: We found that vertebrate H2A.Z-2 is involved in the regulation of the DNA damage response at a very early stage, via the damaged chromatin reorganization required for RAD51 focus formation.},
doi = {10.1016/J.IJROBP.2014.03.031},
journal = {International Journal of Radiation Oncology, Biology and Physics},
number = 4,
volume = 89,
place = {United States},
year = {Tue Jul 15 00:00:00 EDT 2014},
month = {Tue Jul 15 00:00:00 EDT 2014}
}