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Title: Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation

Abstract

Skeletal muscle can regenerate repeatedly due to the presence of resident stem cells, called satellite cells. Because satellite cells are usually quiescent, they must be activated before participating in muscle regeneration in response to stimuli such as injury, overloading, and stretch. Although satellite cell activation is a crucial step in muscle regeneration, little is known of the molecular mechanisms controlling this process. Recent work showed that the bioactive lipid sphingosine-1-phosphate (S1P) plays crucial roles in the activation, proliferation, and differentiation of muscle satellite cells. We investigated the role of growth factors in S1P-mediated satellite cell activation. We found that epidermal growth factor (EGF) in combination with insulin induced proliferation of quiescent undifferentiated mouse myoblast C2C12 cells, which are also known as reserve cells, in serum-free conditions. Sphingosine kinase activity increased when reserve cells were stimulated with EGF. Treatment of reserve cells with the D-erythro-N,N-dimethylsphingosine, Sphingosine Kinase Inhibitor, or siRNA duplexes specific for sphingosine kinase 1, suppressed EGF-induced C2C12 activation. We also present the evidence showing the S1P receptor S1P2 is involved in EGF-induced reserve cell activation. Moreover, we demonstrated a combination of insulin and EGF promoted activation of satellite cells on single myofibers in a manner dependent on SPHK andmore » S1P2. Taken together, our observations show that EGF-induced satellite cell activation is mediated by S1P and its receptor. - Highlights: • EGF in combination with insulin induces proliferation of quiescent C2C12 cells. • Sphingosine kinase activity increases when reserve cells are stimulated with EGF. • EGF-induced activation of reserve cells is dependent on sphingosine kinase and ERK. • The S1P receptor S1P2 is involved in EGF-induced reserve cell activation. • EGF-induced reserve cell activation is mediated by S1P and its receptor.« less

Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22416915
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 326; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALBUMINS; CELL PROLIFERATION; GROWTH FACTORS; INSULIN; MICE; MUSCLES; RECEPTORS; STEM CELLS

Citation Formats

Nagata, Yosuke, E-mail: cynagata@mail.ecc.u-tokyo.ac.jp, Ohashi, Kazuya, Wada, Eiji, Yuasa, Yuki, Shiozuka, Masataka, Nonomura, Yoshiaki, and Matsuda, Ryoichi. Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation. United States: N. p., 2014. Web. doi:10.1016/J.YEXCR.2014.06.009.
Nagata, Yosuke, E-mail: cynagata@mail.ecc.u-tokyo.ac.jp, Ohashi, Kazuya, Wada, Eiji, Yuasa, Yuki, Shiozuka, Masataka, Nonomura, Yoshiaki, & Matsuda, Ryoichi. Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation. United States. doi:10.1016/J.YEXCR.2014.06.009.
Nagata, Yosuke, E-mail: cynagata@mail.ecc.u-tokyo.ac.jp, Ohashi, Kazuya, Wada, Eiji, Yuasa, Yuki, Shiozuka, Masataka, Nonomura, Yoshiaki, and Matsuda, Ryoichi. Fri . "Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation". United States. doi:10.1016/J.YEXCR.2014.06.009.
@article{osti_22416915,
title = {Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation},
author = {Nagata, Yosuke, E-mail: cynagata@mail.ecc.u-tokyo.ac.jp and Ohashi, Kazuya and Wada, Eiji and Yuasa, Yuki and Shiozuka, Masataka and Nonomura, Yoshiaki and Matsuda, Ryoichi},
abstractNote = {Skeletal muscle can regenerate repeatedly due to the presence of resident stem cells, called satellite cells. Because satellite cells are usually quiescent, they must be activated before participating in muscle regeneration in response to stimuli such as injury, overloading, and stretch. Although satellite cell activation is a crucial step in muscle regeneration, little is known of the molecular mechanisms controlling this process. Recent work showed that the bioactive lipid sphingosine-1-phosphate (S1P) plays crucial roles in the activation, proliferation, and differentiation of muscle satellite cells. We investigated the role of growth factors in S1P-mediated satellite cell activation. We found that epidermal growth factor (EGF) in combination with insulin induced proliferation of quiescent undifferentiated mouse myoblast C2C12 cells, which are also known as reserve cells, in serum-free conditions. Sphingosine kinase activity increased when reserve cells were stimulated with EGF. Treatment of reserve cells with the D-erythro-N,N-dimethylsphingosine, Sphingosine Kinase Inhibitor, or siRNA duplexes specific for sphingosine kinase 1, suppressed EGF-induced C2C12 activation. We also present the evidence showing the S1P receptor S1P2 is involved in EGF-induced reserve cell activation. Moreover, we demonstrated a combination of insulin and EGF promoted activation of satellite cells on single myofibers in a manner dependent on SPHK and S1P2. Taken together, our observations show that EGF-induced satellite cell activation is mediated by S1P and its receptor. - Highlights: • EGF in combination with insulin induces proliferation of quiescent C2C12 cells. • Sphingosine kinase activity increases when reserve cells are stimulated with EGF. • EGF-induced activation of reserve cells is dependent on sphingosine kinase and ERK. • The S1P receptor S1P2 is involved in EGF-induced reserve cell activation. • EGF-induced reserve cell activation is mediated by S1P and its receptor.},
doi = {10.1016/J.YEXCR.2014.06.009},
journal = {Experimental Cell Research},
number = 1,
volume = 326,
place = {United States},
year = {Fri Aug 01 00:00:00 EDT 2014},
month = {Fri Aug 01 00:00:00 EDT 2014}
}