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Title: MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19

Abstract

Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which wasmore » similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.« less

Authors:
 [1];  [2];  [1]; ;  [3];  [1];  [2]
  1. Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008 (China)
  2. (China)
  3. Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008 (China)
Publication Date:
OSTI Identifier:
22416887
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 456; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; CORRELATIONS; HUMAN POPULATIONS; IN VITRO; LUNGS; LYMPH NODES; MESSENGER-RNA; MIGRATION; NEOPLASMS; PATHOLOGY; POLYMERASE CHAIN REACTION

Citation Formats

Shan, Nianxi, Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, Shen, Liangfang, Wang, Jun, He, Dan, Duan, Chaojun, E-mail: duancjxy@163.com, and Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19. United States: N. p., 2015. Web. doi:10.1016/J.BBRC.2014.11.093.
Shan, Nianxi, Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, Shen, Liangfang, Wang, Jun, He, Dan, Duan, Chaojun, E-mail: duancjxy@163.com, & Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008. MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19. United States. doi:10.1016/J.BBRC.2014.11.093.
Shan, Nianxi, Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, Shen, Liangfang, Wang, Jun, He, Dan, Duan, Chaojun, E-mail: duancjxy@163.com, and Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008. Fri . "MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19". United States. doi:10.1016/J.BBRC.2014.11.093.
@article{osti_22416887,
title = {MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19},
author = {Shan, Nianxi and Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008 and Shen, Liangfang and Wang, Jun and He, Dan and Duan, Chaojun, E-mail: duancjxy@163.com and Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008},
abstractNote = {Highlights: • Decreased miR-153 and up-regulated ADAM19 are correlated with NSCLC pathology. • MiR-153 inhibits the proliferation and migration and invasion of NSCLC cells in vitro. • ADAM19 is a direct target of miR-153. • ADAM19 is involved in miR-153-suppressed migration and invasion of NSCLC cells. - Abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3′UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.},
doi = {10.1016/J.BBRC.2014.11.093},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 456,
place = {United States},
year = {Fri Jan 02 00:00:00 EST 2015},
month = {Fri Jan 02 00:00:00 EST 2015}
}