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Title: microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells

Abstract

Highlights: • miR-183 was up-regulated in renal cancer tissues. • Inhibition of endogenous miR-183 suppressed renal cancer cell growth and metastasis. • miR-183 increased cell growth and metastasis. • miR-183 regulated renal cancer cell growth and metastasis via directly targeting tumor suppressor protein phosphatase 2A. - Abstract: The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN and A498, respectively, and cell proliferation, Caspase 3/7 activity, colony formation, in vitro migration and invasion were measured; and then the mechanisms of miR-183 regulating was analyzed. We found that miR-183 was up-regulated in renal cancer tissues; inhibition of endogenous miR-183 suppressed in vitro cell proliferation, colony formation, migration, and invasion and stimulated Caspase 3/7 activity; up-regulated miR-183 increased cell growth and metastasis and suppressed Caspase 3/7 activity. We also found that miR-183 directly targeted tumor suppressor, specifically the 3′UTR of three subunits of protein phosphatase 2A (PP2A-Cα, PP2A-Cβ, and PP2A-B56-γ) transcripts, inhibiting their expression and regulated the downstream regulators p21,more » p27, MMP2/3/7 and TIMP1/2/3/4. These results revealed the oncogenes role of miR-183 in renal cancer cells via direct targeting protein phosphatase 2A.« less

Authors:
; ; ; ; ; ; ;
Publication Date:
OSTI Identifier:
22416742
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 452; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; COLONY FORMATION; ELECTROPHORESIS; IN VITRO; INHIBITION; KIDNEYS; METASTASES; MIGRATION; NEOPLASMS; ONCOGENES; ORGANIC FLUORINE COMPOUNDS; PHOSPHATES; POLYMERASE CHAIN REACTION; POLYVINYLS; PROTEINS; RNA; SODIUM; SULFATES

Citation Formats

Qiu, Mingning, E-mail: lcuzfy@163.com, Liu, Lei, E-mail: leiliulab@163.com, Chen, Lieqian, E-mail: lieqianchen@163.com, Tan, Guobin, E-mail: guobintan@163.com, Liang, Ziji, E-mail: zijilianglab@163.com, Wang, Kangning, E-mail: kangningwanglab@163.com, Liu, Jianjun, E-mail: jianjunliulab@163.com, and Chen, Hege, E-mail: hegechen@163.com. microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.08.067.
Qiu, Mingning, E-mail: lcuzfy@163.com, Liu, Lei, E-mail: leiliulab@163.com, Chen, Lieqian, E-mail: lieqianchen@163.com, Tan, Guobin, E-mail: guobintan@163.com, Liang, Ziji, E-mail: zijilianglab@163.com, Wang, Kangning, E-mail: kangningwanglab@163.com, Liu, Jianjun, E-mail: jianjunliulab@163.com, & Chen, Hege, E-mail: hegechen@163.com. microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells. United States. doi:10.1016/J.BBRC.2014.08.067.
Qiu, Mingning, E-mail: lcuzfy@163.com, Liu, Lei, E-mail: leiliulab@163.com, Chen, Lieqian, E-mail: lieqianchen@163.com, Tan, Guobin, E-mail: guobintan@163.com, Liang, Ziji, E-mail: zijilianglab@163.com, Wang, Kangning, E-mail: kangningwanglab@163.com, Liu, Jianjun, E-mail: jianjunliulab@163.com, and Chen, Hege, E-mail: hegechen@163.com. Fri . "microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells". United States. doi:10.1016/J.BBRC.2014.08.067.
@article{osti_22416742,
title = {microRNA-183 plays as oncogenes by increasing cell proliferation, migration and invasion via targeting protein phosphatase 2A in renal cancer cells},
author = {Qiu, Mingning, E-mail: lcuzfy@163.com and Liu, Lei, E-mail: leiliulab@163.com and Chen, Lieqian, E-mail: lieqianchen@163.com and Tan, Guobin, E-mail: guobintan@163.com and Liang, Ziji, E-mail: zijilianglab@163.com and Wang, Kangning, E-mail: kangningwanglab@163.com and Liu, Jianjun, E-mail: jianjunliulab@163.com and Chen, Hege, E-mail: hegechen@163.com},
abstractNote = {Highlights: • miR-183 was up-regulated in renal cancer tissues. • Inhibition of endogenous miR-183 suppressed renal cancer cell growth and metastasis. • miR-183 increased cell growth and metastasis. • miR-183 regulated renal cancer cell growth and metastasis via directly targeting tumor suppressor protein phosphatase 2A. - Abstract: The aim of this study was to investigate the function of miR-183 in renal cancer cells and the mechanisms miR-183 regulates this process. In this study, level of miR-183 in clinical renal cancer specimens was detected by quantitative real-time PCR. miR-183 was up- and down-regulated in two renal cancer cell lines ACHN and A498, respectively, and cell proliferation, Caspase 3/7 activity, colony formation, in vitro migration and invasion were measured; and then the mechanisms of miR-183 regulating was analyzed. We found that miR-183 was up-regulated in renal cancer tissues; inhibition of endogenous miR-183 suppressed in vitro cell proliferation, colony formation, migration, and invasion and stimulated Caspase 3/7 activity; up-regulated miR-183 increased cell growth and metastasis and suppressed Caspase 3/7 activity. We also found that miR-183 directly targeted tumor suppressor, specifically the 3′UTR of three subunits of protein phosphatase 2A (PP2A-Cα, PP2A-Cβ, and PP2A-B56-γ) transcripts, inhibiting their expression and regulated the downstream regulators p21, p27, MMP2/3/7 and TIMP1/2/3/4. These results revealed the oncogenes role of miR-183 in renal cancer cells via direct targeting protein phosphatase 2A.},
doi = {10.1016/J.BBRC.2014.08.067},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 1,
volume = 452,
place = {United States},
year = {2014},
month = {9}
}