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Title: GPER mediated estradiol reduces miR-148a to promote HLA-G expression in breast cancer

Abstract

Highlights: • E2 induces the level of miR-148a in MCF-7 and MDA-MB-231 cells. • GPER mediates the E2-induced increase of miR-148a in MCF-7 and MDA-MB-231 cells. • E2-GPER regulates the expression of HLA-G by miR-148a. - Abstract: Breast cancer is the most common malignant diseases in women. miR-148a plays an important role in regulation of cancer cell proliferation and cancer invasion and down-regulation of miR-148a has been reported in both estrogen receptor (ER) positive and triple-negative (TN) breast cancer. However, the regulation mechanism of miR-148a is unclear. The role of estrogen signaling, a signaling pathway is important in development and progression of breast cancer. Therefore, we speculated that E2 may regulate miR-148a through G-protein-coupled estrogen receptor-1 (GPER). To test our hypothesis, we checked the effects of E2 on miR-148a expression in ER positive breast cancer cell MCF-7 and TN cancer cell MDA-MB-231. Then we used GPER inhibitor G15 to investigate whether GPER is involved in regulation of E2 on miR-148a. Furthermore, we analyzed whether E2 affects the expression of HLA-G, which is a miR-148a target gene through GPER. The results showed that E2 induces the level of miR-148a in MCF-7 and MDA-MB-231 cells, GPER mediates the E2-induced increase in miR-148amore » expression in MCF-7 and MDA-MB-231 cells and E2-GPER regulates the expression of HLA-G by miR-148a. In conclusion, our findings offer important new insights into the ability of estrogenic GPER signaling to trigger HLA-G expression through inhibiting miR-148a that supports immune evasion in breast cancer.« less

Authors:
; ; ;
Publication Date:
OSTI Identifier:
22416710
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 451; Journal Issue: 1; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; ESTRADIOL; GENES; GTP-ASES; HYPOTHESIS; INHIBITION; MAMMARY GLANDS; NEOPLASMS; RECEPTORS; RNA; WOMEN

Citation Formats

Tao, Sifeng, E-mail: taosifeng@aliyun.com, He, Haifei, Chen, Qiang, and Yue, Wenjie. GPER mediated estradiol reduces miR-148a to promote HLA-G expression in breast cancer. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.07.073.
Tao, Sifeng, E-mail: taosifeng@aliyun.com, He, Haifei, Chen, Qiang, & Yue, Wenjie. GPER mediated estradiol reduces miR-148a to promote HLA-G expression in breast cancer. United States. doi:10.1016/J.BBRC.2014.07.073.
Tao, Sifeng, E-mail: taosifeng@aliyun.com, He, Haifei, Chen, Qiang, and Yue, Wenjie. Fri . "GPER mediated estradiol reduces miR-148a to promote HLA-G expression in breast cancer". United States. doi:10.1016/J.BBRC.2014.07.073.
@article{osti_22416710,
title = {GPER mediated estradiol reduces miR-148a to promote HLA-G expression in breast cancer},
author = {Tao, Sifeng, E-mail: taosifeng@aliyun.com and He, Haifei and Chen, Qiang and Yue, Wenjie},
abstractNote = {Highlights: • E2 induces the level of miR-148a in MCF-7 and MDA-MB-231 cells. • GPER mediates the E2-induced increase of miR-148a in MCF-7 and MDA-MB-231 cells. • E2-GPER regulates the expression of HLA-G by miR-148a. - Abstract: Breast cancer is the most common malignant diseases in women. miR-148a plays an important role in regulation of cancer cell proliferation and cancer invasion and down-regulation of miR-148a has been reported in both estrogen receptor (ER) positive and triple-negative (TN) breast cancer. However, the regulation mechanism of miR-148a is unclear. The role of estrogen signaling, a signaling pathway is important in development and progression of breast cancer. Therefore, we speculated that E2 may regulate miR-148a through G-protein-coupled estrogen receptor-1 (GPER). To test our hypothesis, we checked the effects of E2 on miR-148a expression in ER positive breast cancer cell MCF-7 and TN cancer cell MDA-MB-231. Then we used GPER inhibitor G15 to investigate whether GPER is involved in regulation of E2 on miR-148a. Furthermore, we analyzed whether E2 affects the expression of HLA-G, which is a miR-148a target gene through GPER. The results showed that E2 induces the level of miR-148a in MCF-7 and MDA-MB-231 cells, GPER mediates the E2-induced increase in miR-148a expression in MCF-7 and MDA-MB-231 cells and E2-GPER regulates the expression of HLA-G by miR-148a. In conclusion, our findings offer important new insights into the ability of estrogenic GPER signaling to trigger HLA-G expression through inhibiting miR-148a that supports immune evasion in breast cancer.},
doi = {10.1016/J.BBRC.2014.07.073},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 451,
place = {United States},
year = {Fri Aug 15 00:00:00 EDT 2014},
month = {Fri Aug 15 00:00:00 EDT 2014}
}