skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production

Abstract

Highlights: • Romo1 expression is required for constitutive nuclear DNA-binding activity of NF-κB. • Romo1 depletion suppresses tumor growth in vivo. • Romo1 presents a potential therapeutic target for diseases. - Abstract: Deregulation of nuclear factor-κB (NF-κB) and related pathways contribute to tumor cell proliferation and invasion. Mechanisms for constitutive NF-κB activation are not fully explained; however, the underlying defects appear to generate and maintain pro-oxidative conditions. In hepatocellular carcinoma (HCC) tissues, up-regulation of reactive oxygen species modulator 1 (Romo1) correlates positively with tumor size. In the present study, we showed that Romo1 expression is required to maintain constitutive nuclear DNA-binding activity of NF-κB and transcriptional activity through constitutive IκBα phosphorylation. Overexpression of Romo1 promoted p65 nuclear translocation and DNA-binding activity. We also show that Romo1 depletion suppressed anchorage-independent colony formation by HCC cells and suppressed tumor growth in vivo. Based on these findings, Romo1 may be a principal regulatory factor in the maintenance of constitutive NF-κB activation in tumor cells. In the interest of anti-proliferative treatments for cancer, Romo1 may also present a productive target for drug development.

Authors:
; ;
Publication Date:
OSTI Identifier:
22416704
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 450; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; CELL PROLIFERATION; COLONY FORMATION; DEREGULATION; DNA; DRUGS; HEPATOMAS; IN VIVO; OXIDATION; OXYGEN; PHOSPHORYLATION; STRESSES; TRANSLOCATION; TUMOR CELLS

Citation Formats

Chung, Jin Sil, Lee, Sora, and Yoo, Young Do, E-mail: ydy1130@korea.ac.kr. Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.07.059.
Chung, Jin Sil, Lee, Sora, & Yoo, Young Do, E-mail: ydy1130@korea.ac.kr. Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production. United States. doi:10.1016/J.BBRC.2014.07.059.
Chung, Jin Sil, Lee, Sora, and Yoo, Young Do, E-mail: ydy1130@korea.ac.kr. Fri . "Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production". United States. doi:10.1016/J.BBRC.2014.07.059.
@article{osti_22416704,
title = {Constitutive NF-κB activation and tumor-growth promotion by Romo1-mediated reactive oxygen species production},
author = {Chung, Jin Sil and Lee, Sora and Yoo, Young Do, E-mail: ydy1130@korea.ac.kr},
abstractNote = {Highlights: • Romo1 expression is required for constitutive nuclear DNA-binding activity of NF-κB. • Romo1 depletion suppresses tumor growth in vivo. • Romo1 presents a potential therapeutic target for diseases. - Abstract: Deregulation of nuclear factor-κB (NF-κB) and related pathways contribute to tumor cell proliferation and invasion. Mechanisms for constitutive NF-κB activation are not fully explained; however, the underlying defects appear to generate and maintain pro-oxidative conditions. In hepatocellular carcinoma (HCC) tissues, up-regulation of reactive oxygen species modulator 1 (Romo1) correlates positively with tumor size. In the present study, we showed that Romo1 expression is required to maintain constitutive nuclear DNA-binding activity of NF-κB and transcriptional activity through constitutive IκBα phosphorylation. Overexpression of Romo1 promoted p65 nuclear translocation and DNA-binding activity. We also show that Romo1 depletion suppressed anchorage-independent colony formation by HCC cells and suppressed tumor growth in vivo. Based on these findings, Romo1 may be a principal regulatory factor in the maintenance of constitutive NF-κB activation in tumor cells. In the interest of anti-proliferative treatments for cancer, Romo1 may also present a productive target for drug development.},
doi = {10.1016/J.BBRC.2014.07.059},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 450,
place = {United States},
year = {Fri Aug 08 00:00:00 EDT 2014},
month = {Fri Aug 08 00:00:00 EDT 2014}
}