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Title: Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells

Abstract

Highlights: • Two distinct vascular progenitor cells are induced from adult peripheral blood. • ECFCs induce vascular structures in vitro and in vivo. • SMPCs augment the in vitro and in vivo angiogenic potential of ECFCs. • Both cell types have synergistic therapeutic potential in ischemic hindlimb model. - Abstract: Proangiogenic cell therapy using autologous progenitors is a promising strategy for treating ischemic disease. Considering that neovascularization is a harmonized cellular process that involves both endothelial cells and vascular smooth muscle cells, peripheral blood-originating endothelial colony-forming cells (ECFCs) and smooth muscle progenitor cells (SMPCs), which are similar to mature endothelial cells and vascular smooth muscle cells, could be attractive cellular candidates to achieve therapeutic neovascularization. We successfully induced populations of two different vascular progenitor cells (ECFCs and SMPCs) from adult peripheral blood. Both progenitor cell types expressed endothelial-specific or smooth muscle-specific genes and markers, respectively. In a protein array focused on angiogenic cytokines, SMPCs demonstrated significantly higher expression of bFGF, EGF, TIMP2, ENA78, and TIMP1 compared to ECFCs. Conditioned medium from SMPCs and co-culture with SMPCs revealed that SMPCs promoted cell proliferation, migration, and the in vitro angiogenesis of ECFCs. Finally, co-transplantation of ECFCs and SMPCs induced robust in vivomore » neovascularization, as well as improved blood perfusion and tissue repair, in a mouse ischemic hindlimb model. Taken together, we have provided the first evidence of a cell therapy strategy for therapeutic neovascularization using two different types of autologous progenitors (ECFCs and SMPCs) derived from adult peripheral blood.« less

Authors:
;  [1];  [2]; ; ; ;  [1];  [2];  [1]
  1. Department of Cardiology, Cardiovascular Center, College of Medicine, Korea University, Seoul (Korea, Republic of)
  2. Department of Mechanical Engineering, Korea University, Seoul (Korea, Republic of)
Publication Date:
OSTI Identifier:
22416607
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 449; Journal Issue: 4; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADULTS; ANGIOGENESIS; ANIMAL TISSUES; BLOOD; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; GENES; IN VITRO; IN VIVO; ISCHEMIA; LYMPHOKINES; MICE; MIGRATION; MUSCLES; REPAIR; THERAPY

Citation Formats

Joo, Hyung Joon, Seo, Ha-Rim, Jeong, Hyo Eun, Choi, Seung-Cheol, Park, Jae Hyung, Yu, Cheol Woong, Hong, Soon Jun, Chung, Seok, and Lim, Do-Sun. Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells. United States: N. p., 2014. Web. doi:10.1016/J.BBRC.2014.05.061.
Joo, Hyung Joon, Seo, Ha-Rim, Jeong, Hyo Eun, Choi, Seung-Cheol, Park, Jae Hyung, Yu, Cheol Woong, Hong, Soon Jun, Chung, Seok, & Lim, Do-Sun. Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells. United States. https://doi.org/10.1016/J.BBRC.2014.05.061
Joo, Hyung Joon, Seo, Ha-Rim, Jeong, Hyo Eun, Choi, Seung-Cheol, Park, Jae Hyung, Yu, Cheol Woong, Hong, Soon Jun, Chung, Seok, and Lim, Do-Sun. 2014. "Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells". United States. https://doi.org/10.1016/J.BBRC.2014.05.061.
@article{osti_22416607,
title = {Smooth muscle progenitor cells from peripheral blood promote the neovascularization of endothelial colony-forming cells},
author = {Joo, Hyung Joon and Seo, Ha-Rim and Jeong, Hyo Eun and Choi, Seung-Cheol and Park, Jae Hyung and Yu, Cheol Woong and Hong, Soon Jun and Chung, Seok and Lim, Do-Sun},
abstractNote = {Highlights: • Two distinct vascular progenitor cells are induced from adult peripheral blood. • ECFCs induce vascular structures in vitro and in vivo. • SMPCs augment the in vitro and in vivo angiogenic potential of ECFCs. • Both cell types have synergistic therapeutic potential in ischemic hindlimb model. - Abstract: Proangiogenic cell therapy using autologous progenitors is a promising strategy for treating ischemic disease. Considering that neovascularization is a harmonized cellular process that involves both endothelial cells and vascular smooth muscle cells, peripheral blood-originating endothelial colony-forming cells (ECFCs) and smooth muscle progenitor cells (SMPCs), which are similar to mature endothelial cells and vascular smooth muscle cells, could be attractive cellular candidates to achieve therapeutic neovascularization. We successfully induced populations of two different vascular progenitor cells (ECFCs and SMPCs) from adult peripheral blood. Both progenitor cell types expressed endothelial-specific or smooth muscle-specific genes and markers, respectively. In a protein array focused on angiogenic cytokines, SMPCs demonstrated significantly higher expression of bFGF, EGF, TIMP2, ENA78, and TIMP1 compared to ECFCs. Conditioned medium from SMPCs and co-culture with SMPCs revealed that SMPCs promoted cell proliferation, migration, and the in vitro angiogenesis of ECFCs. Finally, co-transplantation of ECFCs and SMPCs induced robust in vivo neovascularization, as well as improved blood perfusion and tissue repair, in a mouse ischemic hindlimb model. Taken together, we have provided the first evidence of a cell therapy strategy for therapeutic neovascularization using two different types of autologous progenitors (ECFCs and SMPCs) derived from adult peripheral blood.},
doi = {10.1016/J.BBRC.2014.05.061},
url = {https://www.osti.gov/biblio/22416607}, journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 4,
volume = 449,
place = {United States},
year = {Fri Jul 11 00:00:00 EDT 2014},
month = {Fri Jul 11 00:00:00 EDT 2014}
}