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Title: Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin

Abstract

Highlights: ► Trichohyalin-like 1 protein is a member of the fused-type S100 protein gene family. ► Specific antibodies against the C-terminus of the TCHHL1 protein were generated. ► TCHHL1 proteins were expressed in the basal layer of the normal epidermis. ► TCHHL1 proteins were strongly expressed in tumor nests of BCC and SCC. ► The expression of TCHHL1 proteins increased in epidermis of psoriasis vulgaris. - Abstract: Trichohyalin-like 1 (TCHHL1) protein is a novel member of the fused-type S100 protein gene family. The deduced amino acid sequence of TCHHL1 contains an EF-hand domain in the N-terminus, one trans-membrane domain and a nuclear localization signal. We generated specific antibodies against the C-terminus of the TCHHL1 protein and examined the expression of TCHHL1 proteins in normal and pathological human skin. An immunohistochemical study showed that TCHHL1 proteins were expressed in the basal layer of the normal epidermis. In addition, signals of TCHHL1 proteins were observed around the nuclei of cultured growing keratinocytes. Accordingly, TCHHL1 mRNA has been detected in normal skin and cultured growing keratinocytes. Furthermore, TCHHL1 proteins were strongly expressed in the peripheral areas of tumor nests in basal cell carcinomas and squamous cell carcinomas. A dramatic increase in the numbermore » of Ki67 positive cells was observed in TCHHL1-expressing areas. The expression of TCHHL1 proteins also increased in non-cancerous hyperproliferative epidermal tissues such as those of psoriasis vulgaris and lichen planus. These findings highlight the possibility that TCHHL1 proteins are expressed in growing keratinocytes of the epidermis and might be associated with the proliferation of keratinocytes.« less

Authors:
 [1];  [1]; ;  [1];  [2];  [1]
  1. Department of Dermatology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194 (Japan)
  2. Department of Integrative Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Sugitani, Toyama 930-0194 (Japan)
Publication Date:
OSTI Identifier:
22416593
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 432; Journal Issue: 1; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; ANTIBODIES; CARCINOMAS; EPIDERMIS; GENES; GLUTATHIONE; HUMAN POPULATIONS; KERATIN; LAYERS; LICHENS; MEMBRANES; MESSENGER-RNA; PHOSPHATES; POLYMERASE CHAIN REACTION; PSORIASIS; TRANSCRIPTION

Citation Formats

Yamakoshi, Takako, Makino, Teruhiko, E-mail: tmakino@med.u-toyama.ac.jp, Ur Rehman, Mati, Yoshihisa, Yoko, Sugimori, Michiya, and Shimizu, Tadamichi, E-mail: shimizut@med.u-toyama.ac.jp. Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin. United States: N. p., 2013. Web. doi:10.1016/J.BBRC.2013.01.084.
Yamakoshi, Takako, Makino, Teruhiko, E-mail: tmakino@med.u-toyama.ac.jp, Ur Rehman, Mati, Yoshihisa, Yoko, Sugimori, Michiya, & Shimizu, Tadamichi, E-mail: shimizut@med.u-toyama.ac.jp. Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin. United States. doi:10.1016/J.BBRC.2013.01.084.
Yamakoshi, Takako, Makino, Teruhiko, E-mail: tmakino@med.u-toyama.ac.jp, Ur Rehman, Mati, Yoshihisa, Yoko, Sugimori, Michiya, and Shimizu, Tadamichi, E-mail: shimizut@med.u-toyama.ac.jp. Fri . "Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin". United States. doi:10.1016/J.BBRC.2013.01.084.
@article{osti_22416593,
title = {Trichohyalin-like 1 protein, a member of fused S100 proteins, is expressed in normal and pathologic human skin},
author = {Yamakoshi, Takako and Makino, Teruhiko, E-mail: tmakino@med.u-toyama.ac.jp and Ur Rehman, Mati and Yoshihisa, Yoko and Sugimori, Michiya and Shimizu, Tadamichi, E-mail: shimizut@med.u-toyama.ac.jp},
abstractNote = {Highlights: ► Trichohyalin-like 1 protein is a member of the fused-type S100 protein gene family. ► Specific antibodies against the C-terminus of the TCHHL1 protein were generated. ► TCHHL1 proteins were expressed in the basal layer of the normal epidermis. ► TCHHL1 proteins were strongly expressed in tumor nests of BCC and SCC. ► The expression of TCHHL1 proteins increased in epidermis of psoriasis vulgaris. - Abstract: Trichohyalin-like 1 (TCHHL1) protein is a novel member of the fused-type S100 protein gene family. The deduced amino acid sequence of TCHHL1 contains an EF-hand domain in the N-terminus, one trans-membrane domain and a nuclear localization signal. We generated specific antibodies against the C-terminus of the TCHHL1 protein and examined the expression of TCHHL1 proteins in normal and pathological human skin. An immunohistochemical study showed that TCHHL1 proteins were expressed in the basal layer of the normal epidermis. In addition, signals of TCHHL1 proteins were observed around the nuclei of cultured growing keratinocytes. Accordingly, TCHHL1 mRNA has been detected in normal skin and cultured growing keratinocytes. Furthermore, TCHHL1 proteins were strongly expressed in the peripheral areas of tumor nests in basal cell carcinomas and squamous cell carcinomas. A dramatic increase in the number of Ki67 positive cells was observed in TCHHL1-expressing areas. The expression of TCHHL1 proteins also increased in non-cancerous hyperproliferative epidermal tissues such as those of psoriasis vulgaris and lichen planus. These findings highlight the possibility that TCHHL1 proteins are expressed in growing keratinocytes of the epidermis and might be associated with the proliferation of keratinocytes.},
doi = {10.1016/J.BBRC.2013.01.084},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 432,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 2013},
month = {Fri Mar 01 00:00:00 EST 2013}
}