Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells

Dong, Yan; Hirane, Miku; Araki, Mutsumi; Fukushima, Nobuyuki; Tsujiuchi, Toshifumi

doi:10.1016/J.BBRC.2014.03.016

Title: Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells

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Abstract

Highlights: • LPA{sub 5} inhibits the cell growth and motile activities of 3T3 cells. • LPA{sub 5} suppresses the cell motile activities stimulated by hydrogen peroxide in 3T3 cells. • Enhancement of LPA{sub 5} on the cell motile activities inhibited by LPA{sub 1} in 3T3 cells. • The expression and activation of Mmp-9 were inhibited by LPA{sub 5} in 3T3 cells. • LPA signaling via LPA{sub 5} acts as a negative regulator of cellular responses in 3T3 cells. - Abstract: Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors (LPA{sub 1}–LPA{sub 6}) mediates a variety of biological functions, including cell migration. Recently, we have reported that LPA{sub 1} inhibited the cell motile activities of mouse fibroblast 3T3 cells. In the present study, to evaluate a role of LPA{sub 5} in cellular responses, Lpar5 knockdown (3T3-L5) cells were generated from 3T3 cells. In cell proliferation assays, LPA markedly stimulated the cell proliferation activities of 3T3-L5 cells, compared with control cells. In cell motility assays with Cell Culture Inserts, the cell motile activities of 3T3-L5 cells were significantly higher than those of control cells. The activity levels of matrix metalloproteinases (MMPs) were measured by gelatin zymography. 3T3-L5 cells stimulated the activation ofmore »« less

Authors:

Dong, Yan; Hirane, Miku; Araki, Mutsumi ^[1]; Fukushima, Nobuyuki ^[2]; Tsujiuchi, Toshifumi ^[1]

Division of Cancer Biology and Bioinformatics, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502 (Japan)
Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502 (Japan)

Publication Date:: Fri Apr 04 00:00:00 EDT 2014

OSTI Identifier:: 22416359

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 446; Journal Issue: 2; Other Information: Copyright (c) 2014 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; BIOLOGICAL FUNCTIONS; CELL CULTURES; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; FIBROBLASTS; GELATIN; GENES; GTP-ASES; HYDROGEN PEROXIDE; MICE; POLYMERASE CHAIN REACTION; RECEPTORS; TRANSCRIPTION

Citation Formats


                    Dong, Yan, Hirane, Miku, Araki, Mutsumi, Fukushima, Nobuyuki, and Tsujiuchi, Toshifumi. Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells.  United States: N. p., 2014. 
        Web.  doi:10.1016/J.BBRC.2014.03.016.

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                    Dong, Yan, Hirane, Miku, Araki, Mutsumi, Fukushima, Nobuyuki, & Tsujiuchi, Toshifumi. Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells.  United States.  https://doi.org/10.1016/J.BBRC.2014.03.016

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                    Dong, Yan, Hirane, Miku, Araki, Mutsumi, Fukushima, Nobuyuki, and Tsujiuchi, Toshifumi. 2014.  
        "Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells".  United States.  https://doi.org/10.1016/J.BBRC.2014.03.016.

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@article{osti_22416359,

  title        = {Lysophosphatidic acid receptor-5 negatively regulates cellular responses in mouse fibroblast 3T3 cells},

  author       = {Dong, Yan and Hirane, Miku and Araki, Mutsumi and Fukushima, Nobuyuki and Tsujiuchi, Toshifumi},

  abstractNote = {Highlights: • LPA{sub 5} inhibits the cell growth and motile activities of 3T3 cells. • LPA{sub 5} suppresses the cell motile activities stimulated by hydrogen peroxide in 3T3 cells. • Enhancement of LPA{sub 5} on the cell motile activities inhibited by LPA{sub 1} in 3T3 cells. • The expression and activation of Mmp-9 were inhibited by LPA{sub 5} in 3T3 cells. • LPA signaling via LPA{sub 5} acts as a negative regulator of cellular responses in 3T3 cells. - Abstract: Lysophosphatidic acid (LPA) signaling via G protein-coupled LPA receptors (LPA{sub 1}–LPA{sub 6}) mediates a variety of biological functions, including cell migration. Recently, we have reported that LPA{sub 1} inhibited the cell motile activities of mouse fibroblast 3T3 cells. In the present study, to evaluate a role of LPA{sub 5} in cellular responses, Lpar5 knockdown (3T3-L5) cells were generated from 3T3 cells. In cell proliferation assays, LPA markedly stimulated the cell proliferation activities of 3T3-L5 cells, compared with control cells. In cell motility assays with Cell Culture Inserts, the cell motile activities of 3T3-L5 cells were significantly higher than those of control cells. The activity levels of matrix metalloproteinases (MMPs) were measured by gelatin zymography. 3T3-L5 cells stimulated the activation of Mmp-2, correlating with the expression levels of Mmp-2 gene. Moreover, to assess the co-effects of LPA{sub 1} and LPA{sub 5} on cell motile activities, Lpar5 knockdown (3T3a1-L5) cells were also established from Lpar1 over-expressing (3T3a1) cells. 3T3a1-L5 cells increased the cell motile activities of 3T3a1 cells, while the cell motile activities of 3T3a1 cells were significantly lower than those of control cells. These results suggest that LPA{sub 5} may act as a negative regulator of cellular responses in mouse fibroblast 3T3 cells, similar to the case for LPA{sub 1}.},

  doi          = {10.1016/J.BBRC.2014.03.016},

  url          = {https://www.osti.gov/biblio/22416359},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 2,

  volume       = 446,

  place        = {United States},

  year         = {Fri Apr 04 00:00:00 EDT 2014},

  month        = {Fri Apr 04 00:00:00 EDT 2014}

}

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