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Title: Solid-immersion fluorescence microscopy with increased emission and super resolution

Abstract

We investigate solid-immersion fluorescence microscopy suitable for super-resolution nanotechnology and biological imaging, and have observed limit of resolution as small as 15 nm with microspheres, mitochondria, and chromatin fibers. We have further observed that fluorescence efficiency increases with excitation power density, implicating appreciable stimulated emission and increased resolution. We discuss potential advantages of the solid-immersion microscopy, including combined use with previously established super-resolution techniques for reaching deeper beyond the conventional diffraction limit.

Authors:
;  [1]; ;  [2];  [3];  [4]
  1. Lincoln Laboratory, Massachusetts Institute of Technology, Lexington, Massachusetts 02420 (United States)
  2. Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)
  3. Whitehead Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States)
  4. Department of Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107 (United States)
Publication Date:
OSTI Identifier:
22399224
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Applied Physics; Journal Volume: 117; Journal Issue: 1; Other Information: (c) 2015 AIP Publishing LLC; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; CHROMATIN; DIFFRACTION; EFFICIENCY; EXCITATION; FIBERS; FLUORESCENCE; MICROSCOPY; MICROSPHERES; MITOCHONDRIA; NANOTECHNOLOGY; POWER DENSITY; RESOLUTION; SOLIDS; STIMULATED EMISSION

Citation Formats

Liau, Z. L., Porter, J. M., Liau, A. A., Chen, J. J., Salmon, W. C., and Sheu, S. S. Solid-immersion fluorescence microscopy with increased emission and super resolution. United States: N. p., 2015. Web. doi:10.1063/1.4905284.
Liau, Z. L., Porter, J. M., Liau, A. A., Chen, J. J., Salmon, W. C., & Sheu, S. S. Solid-immersion fluorescence microscopy with increased emission and super resolution. United States. doi:10.1063/1.4905284.
Liau, Z. L., Porter, J. M., Liau, A. A., Chen, J. J., Salmon, W. C., and Sheu, S. S. Wed . "Solid-immersion fluorescence microscopy with increased emission and super resolution". United States. doi:10.1063/1.4905284.
@article{osti_22399224,
title = {Solid-immersion fluorescence microscopy with increased emission and super resolution},
author = {Liau, Z. L. and Porter, J. M. and Liau, A. A. and Chen, J. J. and Salmon, W. C. and Sheu, S. S.},
abstractNote = {We investigate solid-immersion fluorescence microscopy suitable for super-resolution nanotechnology and biological imaging, and have observed limit of resolution as small as 15 nm with microspheres, mitochondria, and chromatin fibers. We have further observed that fluorescence efficiency increases with excitation power density, implicating appreciable stimulated emission and increased resolution. We discuss potential advantages of the solid-immersion microscopy, including combined use with previously established super-resolution techniques for reaching deeper beyond the conventional diffraction limit.},
doi = {10.1063/1.4905284},
journal = {Journal of Applied Physics},
number = 1,
volume = 117,
place = {United States},
year = {Wed Jan 07 00:00:00 EST 2015},
month = {Wed Jan 07 00:00:00 EST 2015}
}