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Title: The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI{sub 6} octahedral complex to a PtI{sub 3} moiety bound to His15

Abstract

The platinum hexahalides have an octahedral arrangement of six halogen atoms bound to a Pt centre, thus having an octahedral shape that could prove to be useful in interpreting poor electron-density maps. In a detailed characterization, PtI{sub 6} chemically transformed to a square-planar PtI{sub 3} complex bound to the N{sup δ} atom of His15 of HEWL was also observed, which was not observed for PtBr{sub 6} or PtCl{sub 6}. This study examines the binding and chemical stability of the platinum hexahalides K{sub 2}PtCl{sub 6}, K{sub 2}PtBr{sub 6} and K{sub 2}PtI{sub 6} when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI{sub 3} complex bound to the N{sup δ} atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI{sub 6}) or at two sites (PtBr{sub 6} and PtCl{sub 6}). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps asmore » they would be recognisable ‘objects’.« less

Authors:
; ; ; ;  [1]
  1. University of Manchester, Brunswick Street, Manchester M13 9PL (United Kingdom)
Publication Date:
OSTI Identifier:
22375699
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta crystallographica. Section F, Structural biology communications; Journal Volume: 70; Journal Issue: Pt 9; Other Information: PMCID: PMC4157407; PMID: 25195880; PUBLISHER-ID: no5054; OAI: oai:pubmedcentral.nih.gov:4157407; Copyright (c) Tanley et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; ATOMS; CRYSTALS; DENSITY; ELECTRON DENSITY; LYSOZYME; PLATINUM; SHAPE; STABILITY; TRANSFORMATIONS; X-RAY LASERS

Citation Formats

Tanley, Simon W. M., Starkey, Laurina-Victoria, Lamplough, Lucinda, Kaenket, Surasek, and Helliwell, John R., E-mail: john.helliwell@manchester.ac.uk. The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI{sub 6} octahedral complex to a PtI{sub 3} moiety bound to His15. United States: N. p., 2014. Web. doi:10.1107/S2053230X14014009.
Tanley, Simon W. M., Starkey, Laurina-Victoria, Lamplough, Lucinda, Kaenket, Surasek, & Helliwell, John R., E-mail: john.helliwell@manchester.ac.uk. The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI{sub 6} octahedral complex to a PtI{sub 3} moiety bound to His15. United States. doi:10.1107/S2053230X14014009.
Tanley, Simon W. M., Starkey, Laurina-Victoria, Lamplough, Lucinda, Kaenket, Surasek, and Helliwell, John R., E-mail: john.helliwell@manchester.ac.uk. Fri . "The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI{sub 6} octahedral complex to a PtI{sub 3} moiety bound to His15". United States. doi:10.1107/S2053230X14014009.
@article{osti_22375699,
title = {The binding of platinum hexahalides (Cl, Br and I) to hen egg-white lysozyme and the chemical transformation of the PtI{sub 6} octahedral complex to a PtI{sub 3} moiety bound to His15},
author = {Tanley, Simon W. M. and Starkey, Laurina-Victoria and Lamplough, Lucinda and Kaenket, Surasek and Helliwell, John R., E-mail: john.helliwell@manchester.ac.uk},
abstractNote = {The platinum hexahalides have an octahedral arrangement of six halogen atoms bound to a Pt centre, thus having an octahedral shape that could prove to be useful in interpreting poor electron-density maps. In a detailed characterization, PtI{sub 6} chemically transformed to a square-planar PtI{sub 3} complex bound to the N{sup δ} atom of His15 of HEWL was also observed, which was not observed for PtBr{sub 6} or PtCl{sub 6}. This study examines the binding and chemical stability of the platinum hexahalides K{sub 2}PtCl{sub 6}, K{sub 2}PtBr{sub 6} and K{sub 2}PtI{sub 6} when soaked into pre-grown hen egg-white lysozyme (HEWL) crystals as the protein host. Direct comparison of the iodo complex with the chloro and bromo complexes shows that the iodo complex is partly chemically transformed to a square-planar PtI{sub 3} complex bound to the N{sup δ} atom of His15, a chemical behaviour that is not exhibited by the chloro or bromo complexes. Each complex does, however, bind to HEWL in its octahedral form either at one site (PtI{sub 6}) or at two sites (PtBr{sub 6} and PtCl{sub 6}). As heavy-atom derivatives of a protein, the octahedral shape of the hexahalides could be helpful in cases of difficult-to-interpret electron-density maps as they would be recognisable ‘objects’.},
doi = {10.1107/S2053230X14014009},
journal = {Acta crystallographica. Section F, Structural biology communications},
number = Pt 9,
volume = 70,
place = {United States},
year = {Fri Aug 29 00:00:00 EDT 2014},
month = {Fri Aug 29 00:00:00 EDT 2014}
}