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Title: Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum

Abstract

Allene oxide synthase, an atypical cytochrome P450 from Parthenium argentatum, was crystallized and diffraction data were collected to 2.4 Å resolution. Oxylipins are oxygenated derivatives of fatty acids and pivotal signaling molecules in plants and animals. Allene oxide synthase (AOS) is a key cytochrome P450 CYP74 enzyme involved in the biosynthesis of plant oxylipin jasmonates to convert 13(S)-hydroperoxide to allene oxide. Guayule (Parthenium argentatum) AOS, CYP74A2, was expressed in Escherichia coli. Protein was purified using affinity chromatography and size exclusion chromatography, and then crystallized. Two different crystal forms were obtained from 0.2 M (NH{sub 4})H{sub 2}PO{sub 4}, 50% MPD, 0.1 M Tris, pH 8.5 at 277 K using the hanging-drop vapor-diffusion method. Preliminary X-ray analysis was carried out, and the crystals were found to belong to the tetragonal space group I422 with cell parameters a = b = 126.5, c = 163.9 Å, and the monoclinic space group C2 with cell parameters a = 336.5, b = 184.2, c = 159.0 Å, β = 118.6°. Diffraction data were collected to 2.4 Å resolution from a tetragonal form of crystal using a home X-ray source.

Authors:
;  [1];  [2];  [1]
  1. Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401 (United States)
  2. USDA, ARS, Natural Products Utilization Research Unit, PO Box 8048, University, MS 38677 (United States)
Publication Date:
OSTI Identifier:
22360597
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 64; Journal Issue: Pt 7; Other Information: PMCID: PMC2443977; PMID: 18607105; PUBLISHER-ID: ll5155; OAI: oai:pubmedcentral.nih.gov:2443977; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; AFFINITY; CARBOXYLIC ACIDS; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; DIFFUSION; ESCHERICHIA COLI; MOLECULES; OXIDES; OXYGEN; RESOLUTION; SPACE GROUPS; VAPORS; X-RAY SOURCES

Citation Formats

Chang, Zhenzhan, Li, Lenong, Pan, Zhiqiang, and Wang, Xiaoqiang. Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum. United Kingdom: N. p., 2008. Web. doi:10.1107/S1744309108017545.
Chang, Zhenzhan, Li, Lenong, Pan, Zhiqiang, & Wang, Xiaoqiang. Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum. United Kingdom. doi:10.1107/S1744309108017545.
Chang, Zhenzhan, Li, Lenong, Pan, Zhiqiang, and Wang, Xiaoqiang. Tue . "Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum". United Kingdom. doi:10.1107/S1744309108017545.
@article{osti_22360597,
title = {Crystallization and preliminary X-ray analysis of allene oxide synthase, cytochrome P450 CYP74A2, from Parthenium argentatum},
author = {Chang, Zhenzhan and Li, Lenong and Pan, Zhiqiang and Wang, Xiaoqiang},
abstractNote = {Allene oxide synthase, an atypical cytochrome P450 from Parthenium argentatum, was crystallized and diffraction data were collected to 2.4 Å resolution. Oxylipins are oxygenated derivatives of fatty acids and pivotal signaling molecules in plants and animals. Allene oxide synthase (AOS) is a key cytochrome P450 CYP74 enzyme involved in the biosynthesis of plant oxylipin jasmonates to convert 13(S)-hydroperoxide to allene oxide. Guayule (Parthenium argentatum) AOS, CYP74A2, was expressed in Escherichia coli. Protein was purified using affinity chromatography and size exclusion chromatography, and then crystallized. Two different crystal forms were obtained from 0.2 M (NH{sub 4})H{sub 2}PO{sub 4}, 50% MPD, 0.1 M Tris, pH 8.5 at 277 K using the hanging-drop vapor-diffusion method. Preliminary X-ray analysis was carried out, and the crystals were found to belong to the tetragonal space group I422 with cell parameters a = b = 126.5, c = 163.9 Å, and the monoclinic space group C2 with cell parameters a = 336.5, b = 184.2, c = 159.0 Å, β = 118.6°. Diffraction data were collected to 2.4 Å resolution from a tetragonal form of crystal using a home X-ray source.},
doi = {10.1107/S1744309108017545},
journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 7,
volume = 64,
place = {United Kingdom},
year = {2008},
month = {7}
}