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Title: Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex

Abstract

A chitinase isolated from the latex of the tropical species Carica papaya has been crystallized. The addition of N-acetyl-d-glucosamine to the crystallization solution has improved the diffraction quality resolution of the crystal to 1.8 Å resolution. A chitinase isolated from the latex of the tropical species Carica papaya has been purified to homogeneity and crystallized. This enzyme belongs to glycosyl hydrolase family 19 and exhibits exceptional resistance to proteolysis. The initially observed crystals, which diffracted to a resolution of 2.0 Å, were improved through modification of the crystallization protocol. Well ordered crystals were subsequently obtained using N-acetyl-d-glucosamine, the monomer resulting from the hydrolysis of chitin, as an additive to the crystallization solution. Here, the characterization of a chitinase crystal that belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 69.08, b = 44.79, c = 76.73 Å, β = 95.33° and two molecules per asymmetric unit, is reported. Diffraction data were collected to a resolution of 1.8 Å. Structure refinement is currently in progress.

Authors:
 [1];  [2];  [1];  [3];  [1]
  1. Laboratoire de Chimie Générale (CP 206/4), Institut de Pharmacie, Université Libre de Bruxelles (ULB), Campus de la Plaine, Boulevard du Triomphe, B-1050 Bruxelles (Belgium)
  2. Laboratoire de Chimie Générale (CP 609), Faculté de Médecine, Université Libre de Bruxelles (ULB), Campus Erasme, 808 Route de Lennik, B-1070 Bruxelles (Belgium)
  3. CNRS-UMR 8161, Institut de Biologie de Lille, Université de Lille 1-Université de Lille 2-Institut Pasteur de Lille, IFR142, 1 Rue du Professeur Calmette, F-59021 Lille (France)
Publication Date:
OSTI Identifier:
22360560
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 64; Journal Issue: Pt 5; Other Information: PMCID: PMC2376395; PMID: 18453704; PUBLISHER-ID: en5288; OAI: oai:pubmedcentral.nih.gov:2376395; Copyright (c) International Union of Crystallography 2008; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; LATEX; MATHEMATICAL SOLUTIONS; MODIFICATIONS; MOLECULES; RESOLUTION; SOLUTIONS; SPACE GROUPS

Citation Formats

Huet, Joëlle, E-mail: jhuet@ulb.ac.be, Azarkan, Mohamed, Looze, Yvan, Villeret, Vincent, and Wintjens, René, E-mail: jhuet@ulb.ac.be. Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex. United Kingdom: N. p., 2008. Web. doi:10.1107/S1744309108007823.
Huet, Joëlle, E-mail: jhuet@ulb.ac.be, Azarkan, Mohamed, Looze, Yvan, Villeret, Vincent, & Wintjens, René, E-mail: jhuet@ulb.ac.be. Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex. United Kingdom. doi:10.1107/S1744309108007823.
Huet, Joëlle, E-mail: jhuet@ulb.ac.be, Azarkan, Mohamed, Looze, Yvan, Villeret, Vincent, and Wintjens, René, E-mail: jhuet@ulb.ac.be. Thu . "Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex". United Kingdom. doi:10.1107/S1744309108007823.
@article{osti_22360560,
title = {Crystallization and preliminary X-ray analysis of a family 19 glycosyl hydrolase from Carica papaya latex},
author = {Huet, Joëlle, E-mail: jhuet@ulb.ac.be and Azarkan, Mohamed and Looze, Yvan and Villeret, Vincent and Wintjens, René, E-mail: jhuet@ulb.ac.be},
abstractNote = {A chitinase isolated from the latex of the tropical species Carica papaya has been crystallized. The addition of N-acetyl-d-glucosamine to the crystallization solution has improved the diffraction quality resolution of the crystal to 1.8 Å resolution. A chitinase isolated from the latex of the tropical species Carica papaya has been purified to homogeneity and crystallized. This enzyme belongs to glycosyl hydrolase family 19 and exhibits exceptional resistance to proteolysis. The initially observed crystals, which diffracted to a resolution of 2.0 Å, were improved through modification of the crystallization protocol. Well ordered crystals were subsequently obtained using N-acetyl-d-glucosamine, the monomer resulting from the hydrolysis of chitin, as an additive to the crystallization solution. Here, the characterization of a chitinase crystal that belongs to the monoclinic space group P2{sub 1}, with unit-cell parameters a = 69.08, b = 44.79, c = 76.73 Å, β = 95.33° and two molecules per asymmetric unit, is reported. Diffraction data were collected to a resolution of 1.8 Å. Structure refinement is currently in progress.},
doi = {10.1107/S1744309108007823},
journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 5,
volume = 64,
place = {United Kingdom},
year = {2008},
month = {5}
}