skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2

Abstract

The crystallization of the brazil nut allergen Ber e 2 is reported. Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.

Authors:
; ; ;  [1]
  1. Department of Biology, Illinois Institute of Technology, Chicago, IL 60616 (United States)
Publication Date:
OSTI Identifier:
22360434
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 63; Journal Issue: Pt 11; Other Information: PMCID: PMC2339761; PMID: 18007055; PUBLISHER-ID: rp5009; OAI: oai:pubmedcentral.nih.gov:2339761; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BRAZIL; CRYSTALLIZATION; DIFFUSION; GELS; IMPURITIES; MATHEMATICAL SOLUTIONS; MONOCRYSTALS; PRECIPITATION; PROTEINS; SOLUTIONS

Citation Formats

Guo, Feng, Jin, Tengchuan, Howard, Andrew, and Zhang, Yu-Zhu, E-mail: zhangy@iit.edu. Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107051445.
Guo, Feng, Jin, Tengchuan, Howard, Andrew, & Zhang, Yu-Zhu, E-mail: zhangy@iit.edu. Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2. United Kingdom. doi:10.1107/S1744309107051445.
Guo, Feng, Jin, Tengchuan, Howard, Andrew, and Zhang, Yu-Zhu, E-mail: zhangy@iit.edu. Thu . "Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2". United Kingdom. doi:10.1107/S1744309107051445.
@article{osti_22360434,
title = {Purification, crystallization and initial crystallographic characterization of brazil-nut allergen Ber e 2},
author = {Guo, Feng and Jin, Tengchuan and Howard, Andrew and Zhang, Yu-Zhu, E-mail: zhangy@iit.edu},
abstractNote = {The crystallization of the brazil nut allergen Ber e 2 is reported. Peanut and tree-nut allergies have attracted considerable attention because of their frequency and their lifelong persistence. Brazil-nut (Bertholletia excelsa) allergies have been well documented and the 11S legumin-like seed storage protein Ber e 2 (excelsin) is one of the two known brazil-nut allergens. In this study, Ber e 2 was extracted from brazil-nut kernels and purified to high purity by crystalline precipitation and gel-filtration chromatography. Well diffracting single crystals were obtained using the hanging-drop vapour-diffusion method. A molecular-replacement structural solution has been obtained. Refinement of the structure is currently under way.},
doi = {10.1107/S1744309107051445},
journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 11,
volume = 63,
place = {United Kingdom},
year = {2007},
month = {11}
}