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Title: Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain

Abstract

The DIX domain of rat axin has been purified and crystallized. Crystals diffracted to 2.9 Å resolution using synchrotron radiation. Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of β-catenin by glycogen synthase kinase 3β. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6{sub 1} or P6{sub 5}, with unit-cell parameters a = b = 91.49, c = 84.92 Å. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 Å.

Authors:
 [1];  [2];  [3]; ; ; ; ; ;  [1]; ;  [1];  [2]; ;  [4];  [5]; ;  [6];  [1];  [2]
  1. Department of Life Science, Graduate School of Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297 (Japan)
  2. (Japan)
  3. Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502 (Japan)
  4. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto 606-8502 (Japan)
  5. Mitsubishi Pharma Corporation, 1000 Kamoshida-cho, Aoba-ku, Yokohama, Kanagawa 227-0033 (Japan)
  6. Department of Biochemistry, Graduate School of Biomedical Sciences, Hiroshima University, Minami-ku, Hiroshima 734-8551 (Japan)
Publication Date:
OSTI Identifier:
22360349
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 6; Other Information: PMCID: PMC2335085; PMID: 17554179; PUBLISHER-ID: nj5002; OAI: oai:pubmedcentral.nih.gov:2335085; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFUSION; INTERACTIONS; RATS; RESOLUTION; SPACE GROUPS; SYNCHROTRON RADIATION; X-RAY DIFFRACTION

Citation Formats

Shibata, Naoki, E-mail: shibach@sci.u-hyogo.ac.jp, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Tomimoto, Yusuke, Hanamura, Toru, Yamamoto, Ryo, Ueda, Mai, Ueda, Yasufumi, Mizuno, Nobuhiro, Ogata, Hideaki, Komori, Hirofumi, Shomura, Yasuhito, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Kataoka, Michihiko, Shimizu, Sakayu, Kondo, Jun, Yamamoto, Hideki, Kikuchi, Akira, Higuchi, Yoshiki, E-mail: shibach@sci.u-hyogo.ac.jp, and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248. Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107022579.
Shibata, Naoki, E-mail: shibach@sci.u-hyogo.ac.jp, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Tomimoto, Yusuke, Hanamura, Toru, Yamamoto, Ryo, Ueda, Mai, Ueda, Yasufumi, Mizuno, Nobuhiro, Ogata, Hideaki, Komori, Hirofumi, Shomura, Yasuhito, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Kataoka, Michihiko, Shimizu, Sakayu, Kondo, Jun, Yamamoto, Hideki, Kikuchi, Akira, Higuchi, Yoshiki, E-mail: shibach@sci.u-hyogo.ac.jp, & RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248. Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain. United Kingdom. doi:10.1107/S1744309107022579.
Shibata, Naoki, E-mail: shibach@sci.u-hyogo.ac.jp, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Tomimoto, Yusuke, Hanamura, Toru, Yamamoto, Ryo, Ueda, Mai, Ueda, Yasufumi, Mizuno, Nobuhiro, Ogata, Hideaki, Komori, Hirofumi, Shomura, Yasuhito, RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248, Kataoka, Michihiko, Shimizu, Sakayu, Kondo, Jun, Yamamoto, Hideki, Kikuchi, Akira, Higuchi, Yoshiki, E-mail: shibach@sci.u-hyogo.ac.jp, and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248. Fri . "Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain". United Kingdom. doi:10.1107/S1744309107022579.
@article{osti_22360349,
title = {Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain},
author = {Shibata, Naoki, E-mail: shibach@sci.u-hyogo.ac.jp and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248 and Tomimoto, Yusuke and Hanamura, Toru and Yamamoto, Ryo and Ueda, Mai and Ueda, Yasufumi and Mizuno, Nobuhiro and Ogata, Hideaki and Komori, Hirofumi and Shomura, Yasuhito and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248 and Kataoka, Michihiko and Shimizu, Sakayu and Kondo, Jun and Yamamoto, Hideki and Kikuchi, Akira and Higuchi, Yoshiki, E-mail: shibach@sci.u-hyogo.ac.jp and RIKEN SPring-8 Center, 1-1-1 Koto, Sayo-cho, Sayo-gun, Hyogo 679-5248},
abstractNote = {The DIX domain of rat axin has been purified and crystallized. Crystals diffracted to 2.9 Å resolution using synchrotron radiation. Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of β-catenin by glycogen synthase kinase 3β. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6{sub 1} or P6{sub 5}, with unit-cell parameters a = b = 91.49, c = 84.92 Å. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 Å.},
doi = {10.1107/S1744309107022579},
journal = {Acta Crystallographica. Section F},
number = Pt 6,
volume = 63,
place = {United Kingdom},
year = {Fri Jun 01 00:00:00 EDT 2007},
month = {Fri Jun 01 00:00:00 EDT 2007}
}