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Title: Crystallization of human nicotinamide phosphoribosyltransferase

Abstract

Human nicotinamide phosphoribosyltransferase has been crystallized using microseeding methods and X-ray diffraction data have been collected at 2.0 Å resolution. In the NAD biosynthetic pathway, nicotinamide phosphoribosyltransferase (NMPRTase; EC 2.4.2.12) plays an important role in catalyzing the synthesis of nicotinamide mononucleotide from nicotinamide and 5′-phosphoribosyl-1′-pyrophosphate. Because the diffraction pattern of the initally obtained crystals was not suitable for structure analysis, the crystal quality was improved by successive use of the microseeding technique. The resultant crystals diffracted to 2.0 Å resolution. These crystals belonged to space group P21, with unit-cell parameters a = 60.56, b = 106.40, c = 82.78 Å. Here, the crystallization of human NMPRTase is reported in the free form; the crystals should be useful for inhibitor-soaking experiments on the enzyme.

Authors:
; ;  [1];  [1];  [2];  [1]
  1. Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871 (Japan)
  2. (Japan)
Publication Date:
OSTI Identifier:
22360327
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 5; Other Information: PMCID: PMC2335003; PMID: 17565174; PUBLISHER-ID: gj5014; OAI: oai:pubmedcentral.nih.gov:2335003; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; RESOLUTION; SPACE GROUPS; SYNTHESIS; X-RAY DIFFRACTION

Citation Formats

Takahashi, Ryo, Nakamura, Shota, Yoshida, Takuya, Kobayashi, Yuji, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, and Ohkubo, Tadayasu, E-mail: ohkubo@phs.osaka-u.ac.jp. Crystallization of human nicotinamide phosphoribosyltransferase. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107006069.
Takahashi, Ryo, Nakamura, Shota, Yoshida, Takuya, Kobayashi, Yuji, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, & Ohkubo, Tadayasu, E-mail: ohkubo@phs.osaka-u.ac.jp. Crystallization of human nicotinamide phosphoribosyltransferase. United Kingdom. doi:10.1107/S1744309107006069.
Takahashi, Ryo, Nakamura, Shota, Yoshida, Takuya, Kobayashi, Yuji, Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, and Ohkubo, Tadayasu, E-mail: ohkubo@phs.osaka-u.ac.jp. Tue . "Crystallization of human nicotinamide phosphoribosyltransferase". United Kingdom. doi:10.1107/S1744309107006069.
@article{osti_22360327,
title = {Crystallization of human nicotinamide phosphoribosyltransferase},
author = {Takahashi, Ryo and Nakamura, Shota and Yoshida, Takuya and Kobayashi, Yuji and Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094 and Ohkubo, Tadayasu, E-mail: ohkubo@phs.osaka-u.ac.jp},
abstractNote = {Human nicotinamide phosphoribosyltransferase has been crystallized using microseeding methods and X-ray diffraction data have been collected at 2.0 Å resolution. In the NAD biosynthetic pathway, nicotinamide phosphoribosyltransferase (NMPRTase; EC 2.4.2.12) plays an important role in catalyzing the synthesis of nicotinamide mononucleotide from nicotinamide and 5′-phosphoribosyl-1′-pyrophosphate. Because the diffraction pattern of the initally obtained crystals was not suitable for structure analysis, the crystal quality was improved by successive use of the microseeding technique. The resultant crystals diffracted to 2.0 Å resolution. These crystals belonged to space group P21, with unit-cell parameters a = 60.56, b = 106.40, c = 82.78 Å. Here, the crystallization of human NMPRTase is reported in the free form; the crystals should be useful for inhibitor-soaking experiments on the enzyme.},
doi = {10.1107/S1744309107006069},
journal = {Acta Crystallographica. Section F},
number = Pt 5,
volume = 63,
place = {United Kingdom},
year = {Tue May 01 00:00:00 EDT 2007},
month = {Tue May 01 00:00:00 EDT 2007}
}