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Title: Crystallization and preliminary X-ray diffraction data of the rat histone H1{sup 0} globular domain

Abstract

Crystals of the rat histone H1{sup 0} globular domain have been grown in the trigonal space group P3{sub 1}21 and diffracted to a resolution of 1.98 Å. Molecular-replacement solutions were identified and refinement has been started. The linker histones H1 are a family of lysine-rich proteins that associate with the stretch of DNA that enters and exits the nucleosome. The linker histones facilitate the compaction and condensation of chromatin. The globular domain of histone H1{sup 0}, a specific subtype of histone H1, was crystallized at 288 K using the microbatch under silicone oil method with potassium phosphate as a precipitating agent. Diffraction data were collected to a resolution of 1.98 Å. The crystal belongs to the trigonal space group P3{sub 1}21, with unit-cell parameters a = 54.13, b = 54.13, c = 71.99 Å, and contains one molecule per asymmetric unit. The V{sub M} value and solvent content were calculated to be 3.04 Å{sup 3} Da{sup −1} and 59.6%, respectively.

Authors:
; ; ; ; ;  [1]
  1. Department of Biological Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510 (Japan)
Publication Date:
OSTI Identifier:
22360319
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 5; Other Information: PMCID: PMC2334994; PMID: 17565181; PUBLISHER-ID: bw5195; OAI: oai:pubmedcentral.nih.gov:2334994; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DNA; LYSINE; MATHEMATICAL SOLUTIONS; MOLECULES; POTASSIUM PHOSPHATES; RATS; RESOLUTION; SILICON; SOLUTIONS; SOLVENTS; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Matsui, Takuto, Nishimura, Shinsuke, Kijima, Kenichi, Kawasaki, Yasushi, Tashiro, Fumio, and Miura, Shigetoshi, E-mail: smiura@rs.noda.tus.ac.jp. Crystallization and preliminary X-ray diffraction data of the rat histone H1{sup 0} globular domain. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107015357.
Matsui, Takuto, Nishimura, Shinsuke, Kijima, Kenichi, Kawasaki, Yasushi, Tashiro, Fumio, & Miura, Shigetoshi, E-mail: smiura@rs.noda.tus.ac.jp. Crystallization and preliminary X-ray diffraction data of the rat histone H1{sup 0} globular domain. United Kingdom. doi:10.1107/S1744309107015357.
Matsui, Takuto, Nishimura, Shinsuke, Kijima, Kenichi, Kawasaki, Yasushi, Tashiro, Fumio, and Miura, Shigetoshi, E-mail: smiura@rs.noda.tus.ac.jp. Tue . "Crystallization and preliminary X-ray diffraction data of the rat histone H1{sup 0} globular domain". United Kingdom. doi:10.1107/S1744309107015357.
@article{osti_22360319,
title = {Crystallization and preliminary X-ray diffraction data of the rat histone H1{sup 0} globular domain},
author = {Matsui, Takuto and Nishimura, Shinsuke and Kijima, Kenichi and Kawasaki, Yasushi and Tashiro, Fumio and Miura, Shigetoshi, E-mail: smiura@rs.noda.tus.ac.jp},
abstractNote = {Crystals of the rat histone H1{sup 0} globular domain have been grown in the trigonal space group P3{sub 1}21 and diffracted to a resolution of 1.98 Å. Molecular-replacement solutions were identified and refinement has been started. The linker histones H1 are a family of lysine-rich proteins that associate with the stretch of DNA that enters and exits the nucleosome. The linker histones facilitate the compaction and condensation of chromatin. The globular domain of histone H1{sup 0}, a specific subtype of histone H1, was crystallized at 288 K using the microbatch under silicone oil method with potassium phosphate as a precipitating agent. Diffraction data were collected to a resolution of 1.98 Å. The crystal belongs to the trigonal space group P3{sub 1}21, with unit-cell parameters a = 54.13, b = 54.13, c = 71.99 Å, and contains one molecule per asymmetric unit. The V{sub M} value and solvent content were calculated to be 3.04 Å{sup 3} Da{sup −1} and 59.6%, respectively.},
doi = {10.1107/S1744309107015357},
journal = {Acta Crystallographica. Section F},
number = Pt 5,
volume = 63,
place = {United Kingdom},
year = {Tue May 01 00:00:00 EDT 2007},
month = {Tue May 01 00:00:00 EDT 2007}
}