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Title: Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor

Abstract

A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent. A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent. The crystals belong to the tetragonal space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = b = 75.8, c = 150.9 Å. The crystals contain two molecules in the asymmetric unit with a V{sub M} value of 2.5 Å{sup 3} Da{sup −1}. Diffraction was observed to 2.65 Å resolution and a complete data set was collected to 2.9 Å resolution.

Authors:
 [1];  [2]; ;  [1]
  1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667 (India)
  2. Department of Biophysics, All India Institute of Medical Sciences, New Delhi 100 029 (India)
Publication Date:
OSTI Identifier:
22360309
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 4; Other Information: PMCID: PMC2330219; PMID: 17401205; PUBLISHER-ID: ll5104; OAI: oai:pubmedcentral.nih.gov:2330219; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFUSION; MOLECULES; RESOLUTION; SPACE GROUPS; X-RAY DIFFRACTION

Citation Formats

Shee, Chandan, Singh, Tej P., Kumar, Pravindra, E-mail: kumarfbs@iitr.ernet.in, and Sharma, Ashwani K., E-mail: kumarfbs@iitr.ernet.in. Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107011414.
Shee, Chandan, Singh, Tej P., Kumar, Pravindra, E-mail: kumarfbs@iitr.ernet.in, & Sharma, Ashwani K., E-mail: kumarfbs@iitr.ernet.in. Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor. United Kingdom. doi:10.1107/S1744309107011414.
Shee, Chandan, Singh, Tej P., Kumar, Pravindra, E-mail: kumarfbs@iitr.ernet.in, and Sharma, Ashwani K., E-mail: kumarfbs@iitr.ernet.in. Sun . "Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor". United Kingdom. doi:10.1107/S1744309107011414.
@article{osti_22360309,
title = {Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor},
author = {Shee, Chandan and Singh, Tej P. and Kumar, Pravindra, E-mail: kumarfbs@iitr.ernet.in and Sharma, Ashwani K., E-mail: kumarfbs@iitr.ernet.in},
abstractNote = {A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent. A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent. The crystals belong to the tetragonal space group P4{sub 3}2{sub 1}2, with unit-cell parameters a = b = 75.8, c = 150.9 Å. The crystals contain two molecules in the asymmetric unit with a V{sub M} value of 2.5 Å{sup 3} Da{sup −1}. Diffraction was observed to 2.65 Å resolution and a complete data set was collected to 2.9 Å resolution.},
doi = {10.1107/S1744309107011414},
journal = {Acta Crystallographica. Section F},
number = Pt 4,
volume = 63,
place = {United Kingdom},
year = {Sun Apr 01 00:00:00 EDT 2007},
month = {Sun Apr 01 00:00:00 EDT 2007}
}