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Title: Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102

Abstract

The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. A molecular-replacement calculation yielded a satisfactory solution. The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin component of the biphenyl dioxygenase BphA from Pseudomonas sp. strain KKS102, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. The crystal belongs to space group P3{sub 1}21, with unit-cell parameters a = b = 49.6, c = 171.9 Å, and diffracts to a resolution of 1.95 Å. A molecular-replacement calculation using oxidized BphA3 as a search model yielded a satisfactory solution.

Authors:
 [1];  [2];  [3];  [4];  [5]
  1. Japan Biological Information Research Center (JBIRC), Japan Biological Informatics Consortium (JBIC), 2-42 Aomi, Koto-ku, Tokyo 135-0064 (Japan)
  2. Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Hyogo 678-1297 (Japan)
  3. Department of Biomolecular Functional Engineering, University of Ibaraki, 4-12-1 Nakanarusawa, Hitachi, Ibaraki 316-8511 (Japan)
  4. Biological Information Research Center (BIRC), National Institute of Advanced Industrial Science and Technology (AIST), 2-42 Aomi, Koto-ku, Tokyo 135-0064 (Japan)
  5. (JBIRC), Japan Biological Informatics Consortium (JBIC), 2-42 Aomi, Koto-ku, Tokyo 135-0064 (Japan)
Publication Date:
OSTI Identifier:
22360307
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 4; Other Information: PMCID: PMC2330217; PMID: 17401203; PUBLISHER-ID: ll5102; OAI: oai:pubmedcentral.nih.gov:2330217; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; BIPHENYL; CRYSTALLIZATION; CRYSTALS; DIFFUSION; ELECTRON TRANSFER; MATHEMATICAL SOLUTIONS; OXYGEN; RESOLUTION; SOLUTIONS; SPACE GROUPS; STRAINS

Citation Formats

Senda, Miki, Kishigami, Shinya, Kimura, Shigenobu, Senda, Toshiya, E-mail: tsenda@jbirc.aist.go.jp, and Japan Biological Information Research Center. Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107009992.
Senda, Miki, Kishigami, Shinya, Kimura, Shigenobu, Senda, Toshiya, E-mail: tsenda@jbirc.aist.go.jp, & Japan Biological Information Research Center. Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102. United Kingdom. doi:10.1107/S1744309107009992.
Senda, Miki, Kishigami, Shinya, Kimura, Shigenobu, Senda, Toshiya, E-mail: tsenda@jbirc.aist.go.jp, and Japan Biological Information Research Center. Sun . "Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102". United Kingdom. doi:10.1107/S1744309107009992.
@article{osti_22360307,
title = {Crystallization and preliminary X-ray analysis of the reduced Rieske-type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102},
author = {Senda, Miki and Kishigami, Shinya and Kimura, Shigenobu and Senda, Toshiya, E-mail: tsenda@jbirc.aist.go.jp and Japan Biological Information Research Center},
abstractNote = {The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. A molecular-replacement calculation yielded a satisfactory solution. The reduced form of BphA3, a Rieske-type [2Fe–2S] ferredoxin component of the biphenyl dioxygenase BphA from Pseudomonas sp. strain KKS102, was crystallized by the sitting-drop vapour-diffusion method under anaerobic conditions. The crystal belongs to space group P3{sub 1}21, with unit-cell parameters a = b = 49.6, c = 171.9 Å, and diffracts to a resolution of 1.95 Å. A molecular-replacement calculation using oxidized BphA3 as a search model yielded a satisfactory solution.},
doi = {10.1107/S1744309107009992},
journal = {Acta Crystallographica. Section F},
number = Pt 4,
volume = 63,
place = {United Kingdom},
year = {Sun Apr 01 00:00:00 EDT 2007},
month = {Sun Apr 01 00:00:00 EDT 2007}
}