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Title: Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1

Abstract

IREM-1 (immune receptor expressed on myeloid cells 1) is an inhibitory receptor of the immunoglobulin superfamily involved in the functional regulation of myeloid cells. It contains an extracellular region comprising one IgV-like domain. The extracellular IgV-like domain, believed to be involved in target-cell recognition, has been expressed in E. coli, folded in vitro and crystallized. IREM-1 is an inhibitory receptor involved in the functional regulation of myeloid cells. The expression, in vitro folding, purification, crystallization and X-ray data collection of the Ig-V like domain of IREM-1 are reported. X-ray data were collected from a microcrystal (300 × 10 × 10 µm) at 100 K and a diffraction pattern was obtained to 2.6 Å resolution on microfocus beamline ID23-2 at the ESRF. The crystal belongs to space group P3{sub 1}21, with unit-cell parameters a = b = 54.23, c = 72.02 Å, α = γ = 90, β = 120°. Assuming the presence of one molecule per asymmetric unit, V{sub M} (the Matthews coefficient) was calculated to be 1.96 Å{sup 3} Da{sup −1} and the solvent content was estimated to be 37.27%. Determination of the IREM-1 structure will provide insights into its structural requirements for ligand discrimination and binding.

Authors:
 [1]; ; ;  [2];  [3]
  1. Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova (Italy)
  2. European Molecular Biology Laboratory, Grenoble Outstation, Polygon Scientifique, 6 Rue Jules Horowitz, 38000 Grenoble (France)
  3. (Italy)
Publication Date:
OSTI Identifier:
22360287
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 3; Other Information: PMCID: PMC2330191; PMID: 17329815; PUBLISHER-ID: ll5099; OAI: oai:pubmedcentral.nih.gov:2330191; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; EUROPEAN SYNCHROTRON RADIATION FACILITY; IN VITRO; LIGANDS; MOLECULES; RECEPTORS; RESOLUTION; SOLVENTS; SPACE GROUPS

Citation Formats

Dimasi, Nazzareno, E-mail: ndimasi@gmail.com, Flot, David, Dupeux, Florine, Márquez, José A., E-mail: ndimasi@gmail.com, and Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova. Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309107004903.
Dimasi, Nazzareno, E-mail: ndimasi@gmail.com, Flot, David, Dupeux, Florine, Márquez, José A., E-mail: ndimasi@gmail.com, & Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova. Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1. United Kingdom. doi:10.1107/S1744309107004903.
Dimasi, Nazzareno, E-mail: ndimasi@gmail.com, Flot, David, Dupeux, Florine, Márquez, José A., E-mail: ndimasi@gmail.com, and Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova. Thu . "Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1". United Kingdom. doi:10.1107/S1744309107004903.
@article{osti_22360287,
title = {Expression, crystallization and X-ray data collection from microcrystals of the extracellular domain of the human inhibitory receptor expressed on myeloid cells IREM-1},
author = {Dimasi, Nazzareno, E-mail: ndimasi@gmail.com and Flot, David and Dupeux, Florine and Márquez, José A., E-mail: ndimasi@gmail.com and Istituto Giannina Gaslini, Largo Gerolamo Gaslini 5, 16147 Genova},
abstractNote = {IREM-1 (immune receptor expressed on myeloid cells 1) is an inhibitory receptor of the immunoglobulin superfamily involved in the functional regulation of myeloid cells. It contains an extracellular region comprising one IgV-like domain. The extracellular IgV-like domain, believed to be involved in target-cell recognition, has been expressed in E. coli, folded in vitro and crystallized. IREM-1 is an inhibitory receptor involved in the functional regulation of myeloid cells. The expression, in vitro folding, purification, crystallization and X-ray data collection of the Ig-V like domain of IREM-1 are reported. X-ray data were collected from a microcrystal (300 × 10 × 10 µm) at 100 K and a diffraction pattern was obtained to 2.6 Å resolution on microfocus beamline ID23-2 at the ESRF. The crystal belongs to space group P3{sub 1}21, with unit-cell parameters a = b = 54.23, c = 72.02 Å, α = γ = 90, β = 120°. Assuming the presence of one molecule per asymmetric unit, V{sub M} (the Matthews coefficient) was calculated to be 1.96 Å{sup 3} Da{sup −1} and the solvent content was estimated to be 37.27%. Determination of the IREM-1 structure will provide insights into its structural requirements for ligand discrimination and binding.},
doi = {10.1107/S1744309107004903},
journal = {Acta Crystallographica. Section F},
number = Pt 3,
volume = 63,
place = {United Kingdom},
year = {Thu Mar 01 00:00:00 EST 2007},
month = {Thu Mar 01 00:00:00 EST 2007}
}