Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor σ{sup 28} in complex with the σ{sup 28}-binding region of its antisigma factor, FlgM
- Structural Biology Laboratory, Nara Institute of Science and Technology, Keihanna Science City, Nara 630-0192 (Japan)
- Department of Molecular Genetics, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540 (Japan)
A complex of E. coli flagellar and chemotaxis-specific sigma factor σ{sup 28} bound to the σ{sup 28}-binding region of its antisigma factor FlgM was crystallized. Diffraction data were collected to a resolution of 2.7 Å. The sigma 28 kDa (σ{sup 28}) factor is a transcription factor specific for the expression of bacterial flagellar and chemotaxis genes. Its antisigma factor, FlgM, binds σ{sup 28} factor and inhibits its activity as a transcription factor. In this study, crystals of the complex between Escherichia coli σ{sup 28} and the C-terminal σ{sup 28}-binding region of FlgM were obtained. The crystals belong to space group P3{sub 1}21 or P3{sub 2}21, with unit-cell parameters a = b = 106.7 (2), c = 51.74 (3) Å, containing one complex in the crystallographic asymmetric unit. An X-ray intensity data set was collected to a resolution of 2.7 Å.
- OSTI ID:
- 22360278
- Journal Information:
- Acta Crystallographica. Section F, Vol. 63, Issue Pt 3; Other Information: PMCID: PMC2330179; PMID: 17329813; PUBLISHER-ID: en5219; OAI: oai:pubmedcentral.nih.gov:2330179; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); ISSN 1744-3091
- Country of Publication:
- United Kingdom
- Language:
- English
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