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Title: Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis

Abstract

Initiation factor 1 from M. tuberculosis was cloned, expressed, purified and crystallized. A complete data set has been collected to 1.47 Å resolution. Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P2{sub 1}2{sub 1}2, with two molecules per asymmetric unit which are related by translational symmetry.

Authors:
;  [1]
  1. EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg (Germany)
Publication Date:
OSTI Identifier:
22360277
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F
Additional Journal Information:
Journal Volume: 63; Journal Issue: Pt 3; Other Information: PMCID: PMC2330178; PMID: 17329809; PUBLISHER-ID: en5218; OAI: oai:pubmedcentral.nih.gov:2330178; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 1744-3091
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; AFFINITY; CRYSTALLIZATION; CRYSTALS; ESCHERICHIA COLI; MOLECULES; PROTEINS; RESOLUTION; SPACE GROUPS; SYMMETRY

Citation Formats

Hatzopoulos, Georgios N., and Mueller-Dieckmann, Jochen. Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis. United Kingdom: N. p., 2007. Web. doi:10.1107/S174430910700437X.
Hatzopoulos, Georgios N., & Mueller-Dieckmann, Jochen. Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis. United Kingdom. https://doi.org/10.1107/S174430910700437X
Hatzopoulos, Georgios N., and Mueller-Dieckmann, Jochen. 2007. "Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis". United Kingdom. https://doi.org/10.1107/S174430910700437X.
@article{osti_22360277,
title = {Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis},
author = {Hatzopoulos, Georgios N. and Mueller-Dieckmann, Jochen},
abstractNote = {Initiation factor 1 from M. tuberculosis was cloned, expressed, purified and crystallized. A complete data set has been collected to 1.47 Å resolution. Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P2{sub 1}2{sub 1}2, with two molecules per asymmetric unit which are related by translational symmetry.},
doi = {10.1107/S174430910700437X},
url = {https://www.osti.gov/biblio/22360277}, journal = {Acta Crystallographica. Section F},
issn = {1744-3091},
number = Pt 3,
volume = 63,
place = {United Kingdom},
year = {Thu Mar 01 00:00:00 EST 2007},
month = {Thu Mar 01 00:00:00 EST 2007}
}