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Title: Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus

Abstract

DHNA synthetase from G. kaustophilus has been cloned, expressed, purified and crystallized. The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K{sub 2}) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222{sub 1}, with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 Å. The crystal diffracted to a resolution of 2.2 Å. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.

Authors:
;  [1];  [2];  [2];  [3]; ; ;  [2];  [2];  [3];  [2];  [1];  [4];  [2];  [3];  [3];  [5]
  1. Bioinformatics Centre (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012 (India)
  2. RIKEN SPring-8 Center, Harima Institute, 1-1-1 Kouto, Sayo, Hyogo 679-5148 (Japan)
  3. (Japan)
  4. (India)
  5. (Centre of Excellence in Structural Biology and Biocomputing), Indian Institute of Science, Bangalore 560 012 (India)
Publication Date:
OSTI Identifier:
22360270
Resource Type:
Journal Article
Resource Relation:
Journal Name: Acta Crystallographica. Section F; Journal Volume: 63; Journal Issue: Pt 2; Other Information: PMCID: PMC2330136; PMID: 17277450; PUBLISHER-ID: pu5170; OAI: oai:pubmedcentral.nih.gov:2330136; Copyright (c) International Union of Crystallography 2007; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United Kingdom
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; CRYSTALLIZATION; CRYSTALS; MONOMERS; RESOLUTION; SEDIMENTS; SPACE GROUPS

Citation Formats

Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Kuroishi, Chizu, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, and Bioinformatics Centre. Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus. United Kingdom: N. p., 2007. Web. doi:10.1107/S1744309106056521.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Kuroishi, Chizu, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, & Bioinformatics Centre. Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus. United Kingdom. doi:10.1107/S1744309106056521.
Kanaujia, Shankar Prasad, Ranjani, Chellamuthu Vasuki, Jeyakanthan, Jeyaraman, Baba, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Kuroishi, Chizu, Ebihara, Akio, Shinkai, Akeo, Kuramitsu, Seiki, Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Shiro, Yoshitsugu, Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in, Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012, Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in, RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, and Bioinformatics Centre. Thu . "Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus". United Kingdom. doi:10.1107/S1744309106056521.
@article{osti_22360270,
title = {Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus},
author = {Kanaujia, Shankar Prasad and Ranjani, Chellamuthu Vasuki and Jeyakanthan, Jeyaraman and Baba, Seiki and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 and Kuroishi, Chizu and Ebihara, Akio and Shinkai, Akeo and Kuramitsu, Seiki and Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 and Shiro, Yoshitsugu and Sekar, Kanagaraj, E-mail: sekar@serc.iisc.ernet.in and Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore 560 012 and Yokoyama, Shigeyuki, E-mail: sekar@serc.iisc.ernet.in and RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045 and Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 and Bioinformatics Centre},
abstractNote = {DHNA synthetase from G. kaustophilus has been cloned, expressed, purified and crystallized. The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K{sub 2}) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C222{sub 1}, with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 Å. The crystal diffracted to a resolution of 2.2 Å. Preliminary studies and molecular-replacement calculations reveal the presence of three monomers in the asymmetric unit.},
doi = {10.1107/S1744309106056521},
journal = {Acta Crystallographica. Section F},
number = Pt 2,
volume = 63,
place = {United Kingdom},
year = {Thu Feb 01 00:00:00 EST 2007},
month = {Thu Feb 01 00:00:00 EST 2007}
}